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사람 호흡기 상피세포에서 Ginsenoside RB1이 TGF-β1로 유도된 MUC4/5AC 점액 유전자의 발현과 상피-중간엽 전이에 미치는 효과

Ginsenoside Rb1 Attenuates TGF-β1-Induced MUC4/5AC Expression and Epithelial-Mesenchymal Transition in Human Airway Epithelial Cells

조수연|이상재|김용대|최윤석|김준희|송시연|나형균|배창훈|최태영
대한이비인후과학회 (Korean Society of Otorhinolaryngology-Head and Neck Surgery)|2021.04|대한이비인후과학회지 두경부외과학 (Korean Journal of Otorhinolaryngology-Head and Neck Surgery)|제64권 제4호|pp. 232-239|DOI https://doi.org/10.3342/kjorl-hns.2020.00150

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키워드

Ginsenoside Rb1Epithelial-mesenchymal transitionAirway epithelial cellsMUC4MUC5AC .
주제분류이비인후과학

초록

Background and Objectives Ginsenoside Rb1 is the main metabolite of Panax ginseng. Itis known to have many beneficial properties including anti-inflammatory, antitumoral and antioxidanteffects. However, the therapeutic effects of ginenoside Rb1 on inflammatory airwaydiseases have not been elucidated. Therefore, we investigated the effects of ginsenoside Rb1 onthe TGF-β1-induced mucin gene expression and epithelial-mesenchymal transition (EMT) inhuman airway epithelial cells. Materials and Method We evaluated the effects of ginsenoside Rb1 on the changes of MUC4,MUC5AC, occludin, claudin 4, claudin 18, neural (N)-cadherin, and epithelial (E)-cadherin expressionby TGF-β1 in NCI-H292 cells using reverse, real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and western blot. Results TGF-β1 significantly increased MUC4/5AC expression. Rb1 inhibited TGF-β1- inducedMUC4/5AC expression. In addition, TGF-β1 significantly attenuated occludin, claudin 18,and E-cadherin expressions but induced claudin 4 and N-cadherin expressions. On the otherhand, Rb1 reversed changes in the TGF-β1- mediated expressions of cell junction molecules. Conclusion These results suggest that ginsenoside Rb1 attenuates TGF-β1-induced MUC4/5AC expressions and EMT in the human airway epithelial cells. These findings are importantdata demonstrating the potential of ginsenoside Rb1 as a therapeutic agent for inflammatoryairway diseases

Abstract

Background and Objectives Ginsenoside Rb1 is the main metabolite of Panax ginseng. Itis known to have many beneficial properties including anti-inflammatory, antitumoral and antioxidanteffects. However, the therapeutic effects of ginenoside Rb1 on inflammatory airwaydiseases have not been elucidated. Therefore, we investigated the effects of ginsenoside Rb1 onthe TGF-β1-induced mucin gene expression and epithelial-mesenchymal transition (EMT) inhuman airway epithelial cells. Materials and Method We evaluated the effects of ginsenoside Rb1 on the changes of MUC4,MUC5AC, occludin, claudin 4, claudin 18, neural (N)-cadherin, and epithelial (E)-cadherin expressionby TGF-β1 in NCI-H292 cells using reverse, real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and western blot. Results TGF-β1 significantly increased MUC4/5AC expression. Rb1 inhibited TGF-β1- inducedMUC4/5AC expression. In addition, TGF-β1 significantly attenuated occludin, claudin 18,and E-cadherin expressions but induced claudin 4 and N-cadherin expressions. On the otherhand, Rb1 reversed changes in the TGF-β1- mediated expressions of cell junction molecules. Conclusion These results suggest that ginsenoside Rb1 attenuates TGF-β1-induced MUC4/5AC expressions and EMT in the human airway epithelial cells. These findings are importantdata demonstrating the potential of ginsenoside Rb1 as a therapeutic agent for inflammatoryairway diseases

참고문헌 (27)

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