Aucklandia lappa causes cell wall damage in Candida albicans by reducing chitin and (1,3)-é-D-glucan
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The fungal cell wall is a major target of antifungals. In this study, we report the antifungal activity of an ethanol extract from Aucklandia lappa against Candida albicans. We found that the extract caused cell wall injury by decreasing chitin synthesis or assembly and (1,3)-β-D-glucan synthesis. A sorbitol protection assay demonstrated that the minimum inhibitory concentration (MIC) of the A. lappa extract against C. albicans cells increased eight-fold from 0.78 to 6.24 mg/ml in 72 h. Cell aggregates, which indicate damage to the cell wall or membrane, were commonly observed in the A. lappatreated C. albicans cells through microscopic analysis. In addition, the relative fluorescence intensities of the C. albicans cells incubated with the A. lappa extract for 3, 5, and 6 h were 92.1, 84.6, and 79.8%, respectively, compared to the controls, estimated by Calcofluor White binding assay. This result indicates that chitin content was reduced by the A. lappa treatment. Furthermore, synthesis of (1,3)-β-D-glucan polymers was inhibited to 84.3, 79.7, and 70.2% of that of the control treatment following incubation of C. albicans microsomes with the A. lappa extract at a final concentration equal to its MIC, 2× MIC, and 4× MIC, respectively. These findings suggest that the A. lappa ethanol extract may aid the development of a new antifungal to successfully control Candidaassociated disease.
Abstract
The fungal cell wall is a major target of antifungals. In this study, we report the antifungal activity of an ethanol extract from Aucklandia lappa against Candida albicans. We found that the extract caused cell wall injury by decreasing chitin synthesis or assembly and (1,3)-β-D-glucan synthesis. A sorbitol protection assay demonstrated that the minimum inhibitory concentration (MIC) of the A. lappa extract against C. albicans cells increased eight-fold from 0.78 to 6.24 mg/ml in 72 h. Cell aggregates, which indicate damage to the cell wall or membrane, were commonly observed in the A. lappatreated C. albicans cells through microscopic analysis. In addition, the relative fluorescence intensities of the C. albicans cells incubated with the A. lappa extract for 3, 5, and 6 h were 92.1, 84.6, and 79.8%, respectively, compared to the controls, estimated by Calcofluor White binding assay. This result indicates that chitin content was reduced by the A. lappa treatment. Furthermore, synthesis of (1,3)-β-D-glucan polymers was inhibited to 84.3, 79.7, and 70.2% of that of the control treatment following incubation of C. albicans microsomes with the A. lappa extract at a final concentration equal to its MIC, 2× MIC, and 4× MIC, respectively. These findings suggest that the A. lappa ethanol extract may aid the development of a new antifungal to successfully control Candidaassociated disease.
참고문헌 (26)
- Krcmery V, "Non-albicans Candida spp. causing fungaemia: pathogenicity and antifungal resistance", J. Hosp. Infect., 50, pp.243~260 (2002)
- Hachem R, "The changing epidemiology of invasive candidiasis: Candida glabrata and Candida krusei as the leading causes of candidemia in hematologic malignancy", Cancer, 112, pp.2493~2499 (2008)
- Reyna-Beltrán E, "The cell wall of Candida albicans : A proteomics view", IntechOpen Chapter, 4, pp.71~92 (2019)
- Ruiz-Herrera J, "Analysis of the phylogenetic relationships and evolution of the cell walls from yeasts and fungi", FEMS Yeast Res, 10, pp.225~243 (2010)
- Ruiz-Herrera J, "Molecular organization of the cell wall of Candida albicans and its relation to pathogenicity", FEMS Yeast Res, 6, pp.14~29 (2006)
- Chaffin WL, "Candida albicans cell wall proteins", Microbiol. Mol. Biol. Rev, 72, pp.495~544 (2008)
- Carson CF, "Mechanism of action of Melaleuca alternifolia(tea tree)oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assays and electron microscopy", Antimicrob. Agents Chemother, 46, pp.1914~1920 (2002)
- Shapiro RS, "Regulatory circuitry governing fungal development, drug resistance, and disease", Microbiol. Mol. Biol. Rev, 75, pp.213~267 (2011)
- Perumal S, Ramar, "Therapeutic potential of plants as anti-microbials for drug discovery", Evid. Based Complement. Alternat. Med, 7, pp.283~294 (2010)
- Li A, "Preparative isolation and purification of costunolide and dehydrocostuslactone from Aucklandia lappa Decne by high-speed counter-current chromatography", J. Chromatogr. A, 1076, pp.193~197 (2005)
- Kamalpreet LK, "A brief review of remedial uses of Saussurea lappa", J. Pharmacogn. Phytochem, 8, pp.4423~4430 (2019)
- Seo CS, "Anti-allergic effects of sesquiterpene lactones from the root of Aucklandia lappa Decne", Mol. Med. Rep, 12, pp.7789~7795 (2015)
- M27-A3. Reference method for broth dilution antifungal susceptibility testing of yeasts: Approved standard, "Clinical and Laboratory Standards Institute", Clinical and Laboratory Standards Institute, ~ (2008)
- Liu M, "Colorimetric broth microdilution method for the antifungal screening of plant extracts against yeast", Methods, 42, pp.325~329 (2007)
- HEUNG SHICK LEE, "Antifungal Activity of Salvia miltiorrhiza Against Candida albicans Is Associated with the Alteration of Membrane Permeability and (1,3)-β-D-Glucan Synthase Activity", Journal of Microbiology and Biotechnology, 26(3), pp.610~617 (2016), 한국미생물·생명공학회[DOI]
- Shedletzky E, "A microtiter-based fluorescence assay for(1, 3)-β-glucan synthases", Anal. Biochem, 249, pp.88~93 (1997)
- Frost DJ, "A whole-cell Candida albicans assay for the detection of inhibitors towards fungal cell wall synthesis and assembly", J. Antibiot, 48, pp.306~310 (1995)
- Kolotila MP, "Candidacidal activity of macrophages from three mouse strains as demonstrated by a new method : neutral red staining", J. Med. Vet. Mycol, 25, pp.283~290 (1987)
- Lesage G, "Cell wall assembly in Saccharomyces cerevisiae", Microbiol. Mol. Biol. Rev, 70, pp.317~343 (2006)
- Roncero C, "Effect of Calcofluor white and Congo red on fungal cell wall morphogenesis : in vivo activation of chitin polymerization", J. Bacteriol, 163, pp.1180~1185 (1985)
- Heilmann CJ, "Surface stress induces a conserved cell wall stress response in the pathogenic fungus Candida albicans", Eukaryot. Cell, 12, pp.254~264 (2013)
- Lee KK, "Elevated cell wall chitin in Candida albicans confers echinocandin resistance in vivo", Antimicrob. Agents Chemother, 56, pp.208~217 (2012)
- Brasch J, "Inhibition of dermatophytes by optical brighteners", Mycoses, 46, pp.120~125 (2003)
- Barton AA, "Some aspects of cell division in Saccharomyces cerevisiae", J. Gen. Microbiol, 4, pp.84~86 (1950)
- Walker LA, "Stimulation of chitin synthesis rescues Candida albicans from echinocandins", PLoS pathog, 4, pp.e1000040~ (2008)
- Costa‐de‐Oliveira S, "Determination of chitin content in fungal cell wall : an alternative flow cytometric method", Cotometry, 83, pp.324~328 (2013)