Low-Molecular-Weight Collagen Peptide Ameliorates Osteoarthritis Progression through Promoting Extracellular Matrix Synthesis by Chondrocytes in a Rabbit Anterior Cruciate Ligament Transection Model
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
This study examined whether the oral administration of low-molecular-weight collagen peptide (LMCP) containing 3% Gly-Pro-Hyp with >15% tripeptide (Gly-X-Y) content could ameliorate osteoarthritis (OA) progression using a rabbit anterior cruciate ligament transection (ACLT) model of induced OA and chondrocytes isolated from a patient with OA. Oral LMCP administration (100 or 200 mg/kg/day) for 12 weeks ameliorated cartilage damage and reduced the loss of proteoglycan compared to the findings in the ACLT control group, resulting in dose-dependent (p < 0.05) improvements of the OARSI score in hematoxylin & eosin (H&E) and Safranin O staining. In microcomputed tomography analysis, LMCP also significantly (p < 0.05) suppressed the deterioration of the microstructure in tibial subchondral bone during OA progression. The elevation of IL-1β and IL-6 concentrations in synovial fluid following OA induction was dose-dependently (p < 0.05) reduced by LMCP treatment. Furthermore, immunohistochemistry illustrated that LMCP significantly (p < 0.05) upregulated type II collagen and downregulated matrix metalloproteinase-13 in cartilage tissue. Consistent with the in vivo results, LMCP significantly (p < 0.05) increased the mRNA expression of COL2A1 and ACAN in chondrocytes isolated from a patient with OA regardless of the conditions for IL-1β induction. These findings suggest that LMCP has potential as a therapeutic treatment for OA that stimulates cartilage regeneration
Abstract
This study examined whether the oral administration of low-molecular-weight collagen peptide (LMCP) containing 3% Gly-Pro-Hyp with >15% tripeptide (Gly-X-Y) content could ameliorate osteoarthritis (OA) progression using a rabbit anterior cruciate ligament transection (ACLT) model of induced OA and chondrocytes isolated from a patient with OA. Oral LMCP administration (100 or 200 mg/kg/day) for 12 weeks ameliorated cartilage damage and reduced the loss of proteoglycan compared to the findings in the ACLT control group, resulting in dose-dependent (p < 0.05) improvements of the OARSI score in hematoxylin & eosin (H&E) and Safranin O staining. In microcomputed tomography analysis, LMCP also significantly (p < 0.05) suppressed the deterioration of the microstructure in tibial subchondral bone during OA progression. The elevation of IL-1β and IL-6 concentrations in synovial fluid following OA induction was dose-dependently (p < 0.05) reduced by LMCP treatment. Furthermore, immunohistochemistry illustrated that LMCP significantly (p < 0.05) upregulated type II collagen and downregulated matrix metalloproteinase-13 in cartilage tissue. Consistent with the in vivo results, LMCP significantly (p < 0.05) increased the mRNA expression of COL2A1 and ACAN in chondrocytes isolated from a patient with OA regardless of the conditions for IL-1β induction. These findings suggest that LMCP has potential as a therapeutic treatment for OA that stimulates cartilage regeneration
참고문헌 (35)
- Glyn-Jones S, "Osteoarthritis", Lancet, 386, pp.376~387 (2015)
- Le Graverand-Gastineau MP, "Disease modifying osteoarthritis drugs : facing development challenges and choosing molecular targets", Curr. Drug Targets, 11, pp.528~535 (2010)
- Gamble R, "Recommendations for the medical management of osteoarthritis of the hip and knee", Arthritis Rheum, 43, pp.1905~1915 (2000)
- Lee SA, "Chondroprotective effects of aqueous extract of Anthriscus sylvestris leaves on osteoarthritis in vitro and in vivo through MAPKs and NF-κB signaling inhibition", Biomed. Pharmacother, 103, pp.1202~1211 (2018)
- Jeong DH, "Effects of oral glucosamine hydrochloride and mucopolysaccharide protein in a rabbit model of osteoarthritis", Int. J. Rheum. Dis, 21, pp.620~628 (2018)
- Ricci M, "Clinical comparison of oral administration and viscosupplementation of hyaluronic acid(HA)in early knee osteoarthritis", Musculoskelet. Surg, 101, pp.45~49 (2017)
- Jiang Jianqing, "Cardamonin Inhibited IL-1β Induced Injury by Inhibition of NLRP3 Inflammasome via Activating Nrf2", NQO-1 Signaling Pathway in Chondrocyte, 2021, pp.31(6), 794~802[DOI]
- Dong C, "Application of collagen scaffold in tissue engineering : recent advances and new perspectives", Polymers, 8, pp.42~ (2016)
- Song H, "Beneficial effects of collagen hydrolysate : a review on recent developments", Biomed. J. Sci. Tech. Res, 1, pp.1~4 (2017)
- Tsuruoka N, "Promotion by collagen tripeptide of type I collagen gene expression in human osteoblastic cells and fracture healing of rat femur", Biosci. Biotechnol. Biochem, 71, pp.2680~2687 (2007)
- Naraoka T, "Periodic knee injections of collagen tripeptide delay cartilage degeneration in rabbit experimental osteoarthritis", Arthritis Res. Ther, 15, pp.R32~ (2013)
- Min G, "Collagen hydrolysate Gly–Pro–Hyp on osteoblastic proliferation and differentiation of MC3T3-E1 cells", J. Clin. Nurs. Res, 1, pp.40~46 (2017)
- Shigemura Y, "Effect of prolyl-hydroxyproline(Pro–Hyp), a food-derived collagen peptide in human blood, on growth of fibroblasts from mouse skin", J. Agric. Food Chem, 57, pp.444~449 (2009)
- Yamamoto S, "Absorption and plasma kinetics of collagen tripeptide after peroral or intraperitoneal administration in rats", Biosci. Biotechnol. Biochem, 79, pp.2026~2033 (2015)
- Yazaki M, "Oral ingestion of collagen hydrolysate leads to the transportation of highly concentrated Gly–Pro–Hyp and its hydrolyzed form of Pro–Hyp into the bloodstream and skin", J. Agric. Food Chem, 65, pp.2315~2322 (2017)
- Kim DU, "Oral intake of low-molecular-weight collagen peptide improves hydration, elasticity, and wrinkling in human skin : a randomized, double-blind, placebo-controlled study", Nutrients, 10, pp.826~ (2018)
- 김정기, "콜라겐 펩타이드의 피부 광노화 예방 효과", 한국식품과학회지, 41(4), pp.441~445 (2009), 한국식품과학회
- Okawa T, "Oral administration of collagen tripeptide improves dryness and pruritus in the acetone-induced dry skin model", J. Dermatol. Sci, 66, pp.136~143 (2012)
- Hee Bong Pyun, "Effects of Collagen Tripeptide Supplement on Photoaging and Epidermal Skin Barrier in UVB-exposed Hairless Mice", Preventive Nutrition and Food Science, 17(4), pp.245~253 (2012), 한국식품영양과학회[DOI]
- Liu Z, "A novel rabbit model of early osteoarthritis exhibits gradual cartilage degeneration after medial collateral ligament transection outside the joint capsule", Sci. Rep, 6, pp.34423~ (2016)
- Pritzker KP, "Osteoarthritis cartilage histopathology : grading and staging", Osteoarthr. Cartil, 14, pp.13~29 (2006)
- Laverty S, "The OARSI histopathology initiative–recommendations for histological assessments of osteoarthritis in the rabbit", Osteoarthritis Cartilage, 18(Suppl 3), pp.S24~S34 (2010)
- Kwon JY, "Kartogenin inhibits pain behavior, chondrocyte inflammation, and attenuates osteoarthritis progression in mice through induction of IL-10", Sci. Rep, 8, pp.13832~ (2018)
- Pritzker KP, "Articular Cartilage of the Knee", pp.371~388 (2020), Springer
- Gao Y, "The ECM-cell interaction of cartilage extracellular matrix on chondrocytes", Biomed. Res. Int, 2014, pp.648459~ (2014)
- Yang CY, "ADAMTS and ADAM metalloproteinases in osteoarthritis–looking beyond the ‘usual suspects’", Osteoarthritis Cartilage, 25, pp.1000~1009 (2017)
- Kiani C, "Structure and function of aggrecan", Cell Res, 12, pp.19~32 (2002)
- Sandell LJ, "Articular cartilage and changes in arthritis : cell biology of osteoarthritis", Arthritis Res. Ther, 3, pp.1~7 (2001)
- Grynpas MD, "Subchondral bone in osteoarthritis", Calcif. Tissue Int, 49, pp.20~26 (1991)
- Imhof H, "Subchondral bone and cartilage disease : a rediscovered functional unit", Invest. Radiol, 35, pp.581~588 (2000)
- König D, "Specific collagen peptides improve bone mineral density and bone markers in postmenopausal women—a randomized controlled study", Nutrients, 10, pp.97~ (2018)
- Klocke R, "Cartilage turnover and intra-articular corticosteroid injections in knee osteoarthritis", Rheumatol. Int, 38, pp.455~459 (2018)
- Pereira H, "Emerging concepts in treating cartilage, osteochondral defects, and osteoarthritis of the knee and ankle", Adv. Exp. Med. Biol, 1059, pp.25~62 (2018)
- Hu ZC, "The protective effect of magnolol in osteoarthritis : in vitro and in vivo studies", Front. Pharmacol, 10, pp.393~ (2019)
- Postlethwaite AE, "Chemotactic attraction of human fibroblasts to type I, II, and III collagens and collagenderived peptides", Proc. Natl. Acad. Sci. USA, 75, pp.871~875 (1978)
