Relationship between Pulmonary Surfactant Protein and Lipid Peroxidation in Lung Injury due to Paraquat Intoxication in Rats
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Background : Pulmonary damage resulting from lipid peroxidation is a principal effect of paraquat intoxication. The host-defense functions of surfactant are known to be mediated by the surfactant proteins A and D (SP-A and SP-D, respectively). The primary objective of this study was to evaluate the variations over time in levels of surfactant protein and lipid peroxidation (LPO) in lung tissue following free-radical-induced injury. Methods : 42 adult, male, Sprague-Dawley rats were administered intraperitoneal injections of paraquat (35 mg/kg body weight). SP-A and SP-D levels were determined via Western blot. LPO in the left lung homogenate was measured via analyses of the levels of thiobarbituric acid-reactive substances. Results : LPO levels peaked at 6 hours, with no associated histological changes. SP-D levels increased until hour 12 and declined until hour 48; SP-D levels subsequently began to increase again, peaking at hour 72. SP-A levels peaked at hour 6, declining thereafter. Conclusions : We suggest that in the early phase of paraquat injury, SP-D levels reflect alveolar damage and that de novo synthesis of SP-D takes 72 hours. Levels of SP-A, on the other hand, reflect abnormalities in the surfactant system in the late stage of paraquat intoxication. Surfactant proteins may play a role in protecting the lungs from reactive oxygen injury. A time-dependent variation has been observed in the levels of surfactant proteins A and D following paraquat injury, and it has been suggested that these proteins play a role in the protection of lung tissue against ROS-induced injuries.
Abstract
Background : Pulmonary damage resulting from lipid peroxidation is a principal effect of paraquat intoxication. The host-defense functions of surfactant are known to be mediated by the surfactant proteins A and D (SP-A and SP-D, respectively). The primary objective of this study was to evaluate the variations over time in levels of surfactant protein and lipid peroxidation (LPO) in lung tissue following free-radical-induced injury. Methods : 42 adult, male, Sprague-Dawley rats were administered intraperitoneal injections of paraquat (35 mg/kg body weight). SP-A and SP-D levels were determined via Western blot. LPO in the left lung homogenate was measured via analyses of the levels of thiobarbituric acid-reactive substances. Results : LPO levels peaked at 6 hours, with no associated histological changes. SP-D levels increased until hour 12 and declined until hour 48; SP-D levels subsequently began to increase again, peaking at hour 72. SP-A levels peaked at hour 6, declining thereafter. Conclusions : We suggest that in the early phase of paraquat injury, SP-D levels reflect alveolar damage and that de novo synthesis of SP-D takes 72 hours. Levels of SP-A, on the other hand, reflect abnormalities in the surfactant system in the late stage of paraquat intoxication. Surfactant proteins may play a role in protecting the lungs from reactive oxygen injury. A time-dependent variation has been observed in the levels of surfactant proteins A and D following paraquat injury, and it has been suggested that these proteins play a role in the protection of lung tissue against ROS-induced injuries.
참고문헌 (29)
- "Evidence for energy-dependent accumulation of paraquat into rat lung", pp.314~252 315 (1974)
- "Differential paraquat uptake and redox kinetics of rat granular pneumocytes and alveolar macrophages", pp.428~433 (1982)
- "Clinical management of poisoning and drug overdose", pp.1088~1103 (1990), Winchester JF Winchester JF
- "The loss of pulmonary surfactant in paraquat poisoning a model of the study of the respiratory distress syndrome", pp.366~369 (1967)
- "Collectins and innate immunity in the lung", 2, pp.273~278 (2000)
- "Pulmonary collectins and innate host defense of the lung", 3, pp.161~166 (2001)
- "Altered surfactant homeostasis and alveolar type II cell morphology in mice lacking surfactant protein D", 95, pp.11869~11874 (1998)
- "Increased metalloproteinase activity, oxidant production, and emphysema in surfactant protein D gene-inactivated mice", 97, pp.5972~5977 (2000)
- "Upregulation of acidic fibroblast growth factor during development of experimental lung fibrosis", 273, pp.L451~L458 (1997)
- "Experimental pulmonary fibrosis induced by paraquat plus oxygen in rats", pp.147~166 (1989)
- "Determination of total protein", pp.95~119 (1983)
- "Pulmonary effects of the herbicide paraquat studied 3 days after injection in rats", pp.268~273 (1973)
- "Pulmonary effects of paraquat in the first day after injection", pp.1217~1223 (1975)
- "Experimental respiratory distress induced by paraquat", pp.239~244 (1971)
- "The toxicity of paraquat", pp.1~17 (1988)
- "Herbicide", pp.1393~1410 (2002), USA, McGraw-Hill
- "Lung toxicity of paraquat in the rat", 60, pp.477~487 (2000)
- "Alterations in SP-B and SP-C expression in neonatal lung disease", 66, pp.601~623 (2004)
- "Immunoregulatory functions of surfactant proteins", 5, pp.58~68 (2005)
- "Immunomodulatory functions of surfactant", 77, pp.931~962 (1997)
- "Pulmonary surfactant- associated protein A enhances the surface activity of lipid extract surfactant and reverses inhibition by blood proteins in vitro", pp.8424~8429 (1990)
- "Reconstitution of tubular myelin from synthetic lipids and proteins associated with pig pulmonary surfactant", pp.75~81 (1989)
- "Inhibition of lung calcium- independent phospholipase A2 by surfactant protein A" (1994)
- "A role for phospholipase A2 in ARDS pathogenesis", 5, pp.244~249 (1999)
- "Pulmonary surfactant proteins A and D are potent endogenous inhibitors of lipid peroxidation and oxidative cellular injury", 275, pp.38848~38855 (2000)
- "Surfactant protein D reduces alveolar macrophage apoptosis in vivo", 169, pp.2892~2899 (2002)
- "Serum SP-D is a marker of lung injury in rats", 282, pp.L824~L832 (2002)
- "Bronchoalveolar lavage with a diluted surfactant suspension prior to surfactant instillation improves the effectiveness of surfactant therapy in experimental acute respiratory distress syndrome (ARDS)", 24, pp.494~500 (1998)
- "Effect of exogenous surfactant instillation on experimental acute lung injury" (1846)