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Proteomic differences with and without ozoneexposure in a smoking-induced emphysema lung model

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키워드

주제분류내과학

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Background/Aims: Acute exacerbations in chronic obstructive pulmonary diseasemay be related to air pollution, of which ozone is an important constituent. In this study, we investigated the protein profiles associated with ozone-inducedexacerbations in a smoking-induced emphysema model. Methods: Mice were divided into the following groups: group I, no smoking andno ozone (NS + NO); group II, no smoking and ozone (NS + O); group III, smokingand no ozone (S + NO); and group IV, smoking and ozone (S + O). Bronchoalveolarlavage, the mean linear intercept (MLI) on hematoxylin and eosin staining, nano-liquid chromatography-tandem mass spectrometry (LC-MS/MS), and Westernblotting analyses were performed. Results: The MLIs of groups III (S + NO) and IV (S + O) (45 ± 2 and 44 ± 3 μm, respectively)were significantly higher than those of groups I (NS + NO) and II (NS+ O) (26 ± 2 and 23 ± 2 μm, respectively; p < 0.05). Fourteen spots that showed significantlydifferent intensities on image analyses of two-dimensional (2D) proteinelectrophoresis in group I (NS + NO) were identified by LC-MS/MS. The levels ofsix proteins were higher in group IV (S + O). The levels of vimentin, lactate dehydrogenaseA, and triose phosphate isomerase were decreased by both smokingand ozone treatment in Western blotting and proteomic analyses. In contrast,TBC1 domain family 5 (TBC1D5) and lamin A were increased by both smokingand ozone treatment. Conclusions: TBC1D5 could be a biomarker of ozone-induced lung injury in emphysema.

Abstract

Background/Aims: Acute exacerbations in chronic obstructive pulmonary diseasemay be related to air pollution, of which ozone is an important constituent. In this study, we investigated the protein profiles associated with ozone-inducedexacerbations in a smoking-induced emphysema model. Methods: Mice were divided into the following groups: group I, no smoking andno ozone (NS + NO); group II, no smoking and ozone (NS + O); group III, smokingand no ozone (S + NO); and group IV, smoking and ozone (S + O). Bronchoalveolarlavage, the mean linear intercept (MLI) on hematoxylin and eosin staining, nano-liquid chromatography-tandem mass spectrometry (LC-MS/MS), and Westernblotting analyses were performed. Results: The MLIs of groups III (S + NO) and IV (S + O) (45 ± 2 and 44 ± 3 μm, respectively)were significantly higher than those of groups I (NS + NO) and II (NS+ O) (26 ± 2 and 23 ± 2 μm, respectively; p < 0.05). Fourteen spots that showed significantlydifferent intensities on image analyses of two-dimensional (2D) proteinelectrophoresis in group I (NS + NO) were identified by LC-MS/MS. The levels ofsix proteins were higher in group IV (S + O). The levels of vimentin, lactate dehydrogenaseA, and triose phosphate isomerase were decreased by both smokingand ozone treatment in Western blotting and proteomic analyses. In contrast,TBC1 domain family 5 (TBC1D5) and lamin A were increased by both smokingand ozone treatment. Conclusions: TBC1D5 could be a biomarker of ozone-induced lung injury in emphysema.

참고문헌 (37)

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