Association of biofilm production with colonization among clinical isolates of Acinetobacter baumannii
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Background/Aims: The pathogen Acinetobacter baumannii is increasingly causing healthcare-associated infections worldwide, particularly in intensive care units. Biofilm formation, a factor contributing to the virulence of A. baumannii, is associated with long-term persistence in hospital environments. The present study investigates the clinical impact of biofilm production on colonization and acquisition after patient admission. Methods: Forty-nine A. baumannii isolates were obtained between August and November 2013 from Keimyung University Dongsan Medical Center, Daegu, Korea. All isolates were obtained from sputum samples of new patients infected or colonized by A. baumannii. The microtiter plate assay was used to determine biofilm formation. Results: Twenty-four A. baumannii isolates (48%) demonstrated enhanced biofilm formation capacity than that of the standard A. baumannii strain (ATCC 19606). All isolates were resistant to carbapenem, 38 isolates (77%) were collected from patients in an intensive care unit, and 47 isolates (95%) were from patients who had been exposed to antibiotics in the previous month. The median duration of colonization was longer for biofilm-producing isolates than that of the biofilm non-biofilm producing isolates (18 days vs. 12 days, p < 0.05). Simultaneous colonization with other bacteria was more common for biofilm-producing isolates than that for the non-biofilm producing isolates. The most prevalent co-colonizing bacteria was Staphylococcus aureus. Conclusions: Biofilm-producing isolates seem to colonize the respiratory tract for longer durations than the non-biofilm producing isolates. During colonization, biofilm producers promote co-colonization by other bacteria, particularly S. aureus. Additional research is required to determine possible links between biofilm formation and nosocomial infection.
Abstract
Background/Aims: The pathogen Acinetobacter baumannii is increasingly causing healthcare-associated infections worldwide, particularly in intensive care units. Biofilm formation, a factor contributing to the virulence of A. baumannii, is associated with long-term persistence in hospital environments. The present study investigates the clinical impact of biofilm production on colonization and acquisition after patient admission. Methods: Forty-nine A. baumannii isolates were obtained between August and November 2013 from Keimyung University Dongsan Medical Center, Daegu, Korea. All isolates were obtained from sputum samples of new patients infected or colonized by A. baumannii. The microtiter plate assay was used to determine biofilm formation. Results: Twenty-four A. baumannii isolates (48%) demonstrated enhanced biofilm formation capacity than that of the standard A. baumannii strain (ATCC 19606). All isolates were resistant to carbapenem, 38 isolates (77%) were collected from patients in an intensive care unit, and 47 isolates (95%) were from patients who had been exposed to antibiotics in the previous month. The median duration of colonization was longer for biofilm-producing isolates than that of the biofilm non-biofilm producing isolates (18 days vs. 12 days, p < 0.05). Simultaneous colonization with other bacteria was more common for biofilm-producing isolates than that for the non-biofilm producing isolates. The most prevalent co-colonizing bacteria was Staphylococcus aureus. Conclusions: Biofilm-producing isolates seem to colonize the respiratory tract for longer durations than the non-biofilm producing isolates. During colonization, biofilm producers promote co-colonization by other bacteria, particularly S. aureus. Additional research is required to determine possible links between biofilm formation and nosocomial infection.
참고문헌 (18)
- Peleg AY, "Acinetobacter baumannii:emergence of a successful pathogen", Clin Microbiol Rev, 21, pp.538~582 (2008)
- Chung DR, "High prevalence of multidrug-resistant nonfermenters in hospital-acquired pneumonia in Asia", Am J Respir Crit Care Med, 184, pp.1409~1417 (2011)
- Talbot GH, "Bad bugs need drugs: an update on the development pipeline from the Antimicrobial Availability Task Force of the Infectious Diseases Society of America", Clin Infect Dis, 42, pp.657~668 (2006)
- Lee HW, "Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces", Clin Microbiol Infect, 14, pp.49~54 (2008)
- Espinal P, "Effect of biofilm formation on the survival of Acinetobacter baumannii on dry surfaces", J Hosp Infect, 80, pp.56~60 (2012)
- Da Silva G, "Identification of widespread, closely related Acinetobacter baumannii isolates in Portugal as a subgroup of European clone II", Clin Microbiol Infect, 13, pp.190~195 (2007)
- Kaliterna V, "The ability of biofilm formation in clinical isolates of Acinetobacter baumannii belonging to two different European clones causing outbreaks in the Split University Hospital, Croatia", J Chemother, 25, pp.60~62 (2013)
- Arvaniti K, "The importance of colonization pressure in multiresistant Acinetobacter baumannii acquisition in a Greek intensive care unit", Crit Care, 16, pp.R102~ (2012)
- Sheng WH, "A multicenter study of risk factors and outcome of hospitalized patients with infections due to carbapenem-resistant Acinetobacter baumannii", Int J Infect Dis, 14, pp.e764~e769 (2010)
- Horan TC, "CDC", NHSN surveillance definition of health care-associated infection and criteria for specific types of infections in the acute care setting, 2008, pp.36, 309~332
- Pettit RK, "Microplate Alamar blue assay for Staphylococcus epidermidis biofilm susceptibility testing", Antimicrob Agents Chemother, 49, pp.2612~2617 (2005)
- Tomaras AP, "Attachment to and biofilm formation on abiotic surfaces by Acinetobacter baumannii: involvement of a novel chaperone-usher pili assembly system", Microbiology, 149(Pt 12), pp.3473~3484 (2003)
- Kim HA, "Biofilm formation and colistin susceptibility of Acinetobacter baumannii isolated from Korean nosocomial samples", Microb Drug Resist, 21, pp.452~457 (2015)
- Lim CJ, "Prevalence of multidrug-resistant organisms and risk factors for carriage in long-term care facilities: a nested case-control study", J Antimicrob Chemother, 69, pp.1972~1980 (2014)
- Pneumatikos IA, "Ventilator-associated pneumonia or endotracheal tube-associated pneumonia? An approach to the pathogenesis and preventive strategies emphasizing the importance of endotracheal tube", Anesthesiology, 110, pp.673~680 (2009)
- Gil-Perotin S, "Implications of endotracheal tube biofilm in ventilator-associated pneumonia response: a state of concept", Crit Care, 16, pp.R93~ (2012)
- Zago CE, "Dynamics of biofilm formation and the interaction between Candida albicans and methicillin-susceptible (MSSA) and -resistant Staphylococcus aureus (MRSA)", PLoS One, 10, pp.e0123206~ (2015)
- Gaddy JA, "The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells", Infect Immun, 77, pp.3150~3160 (2009)