Effect of the Position of Immobilization Upon the Tensile Properties in Injured Achilles Tendon of Rat
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective To examine the effect of the posture of immobilization upon the tensile properties in injured Achilles tendon of rat for an initial period of immobilization.Methods Forty-two Sprague-Dawley rats were used in the present study. Eighteen rats received a total tenotomy of the right Achilles tendon to mimic total rupture and were divided into three groups comprising of 6 rats each. Ankles of group A were immobilized at 60° of plantarflexion. Ankles of group B were immobilized at neutral position. Whereas, those of group C were immobilized at 60° of dorsiflexion. Other 18 rats received hemitenotomy to mimic partial rupture and were divided into three groups. The remaining 6 rats were kept free as control. After 14 days, we dissected the tendons and analyzed maximum force, stiffness, and energy uptake during pulling of the tendons until they ruptured. The tendons of 6 rats in each group and control were reserved for histology. Picrosirius staining was done for the analysis of collagen organization.Results In total tenotomy, tensile properties were significantly different between the control and the intervention groups (p<0.05). Group C showed relatively higher values than the groups A and B with respect to tensile properties (p>0.05). In partial tenotomy, tensile properties were significantly different between the control and the intervention groups (p<0.05). Group C showed significantly higher value than other intervention groups in terms of maximum force and energy uptake (p<0.05). The semiquantitative histologic grading scores were assigned for collagen organization. The scores for dorsiflexion posture were higher than the ones for plantarflexion.Conclusion Dorsiflexion posture in partial ruptured Achilles tendon showed better functional recovery than other immobilized postures. In total ruptured case, the tensile properties showed increasing tendency in dorsiflexion posture.
Abstract
Objective To examine the effect of the posture of immobilization upon the tensile properties in injured Achilles tendon of rat for an initial period of immobilization.Methods Forty-two Sprague-Dawley rats were used in the present study. Eighteen rats received a total tenotomy of the right Achilles tendon to mimic total rupture and were divided into three groups comprising of 6 rats each. Ankles of group A were immobilized at 60° of plantarflexion. Ankles of group B were immobilized at neutral position. Whereas, those of group C were immobilized at 60° of dorsiflexion. Other 18 rats received hemitenotomy to mimic partial rupture and were divided into three groups. The remaining 6 rats were kept free as control. After 14 days, we dissected the tendons and analyzed maximum force, stiffness, and energy uptake during pulling of the tendons until they ruptured. The tendons of 6 rats in each group and control were reserved for histology. Picrosirius staining was done for the analysis of collagen organization.Results In total tenotomy, tensile properties were significantly different between the control and the intervention groups (p<0.05). Group C showed relatively higher values than the groups A and B with respect to tensile properties (p>0.05). In partial tenotomy, tensile properties were significantly different between the control and the intervention groups (p<0.05). Group C showed significantly higher value than other intervention groups in terms of maximum force and energy uptake (p<0.05). The semiquantitative histologic grading scores were assigned for collagen organization. The scores for dorsiflexion posture were higher than the ones for plantarflexion.Conclusion Dorsiflexion posture in partial ruptured Achilles tendon showed better functional recovery than other immobilized postures. In total ruptured case, the tensile properties showed increasing tendency in dorsiflexion posture.
참고문헌 (26)
- Thompson J., "Acute and chronic Achilles tendon ruptures in athletes", Clin Podiatr Med Surg, 28, pp.117~135 (2011)
- Matsumoto F., "Mechanical effects of immobilization on the Achilles’ tendon", Arch Phys Med Rehabil, 84, pp.662~667 (2003)
- 이종하, "흰쥐 아킬레스건 손상 후 부신피질 호르몬 국소 주사와 체중 부하의 영향", Annals of Rehabilitation Medicine, 26(2), pp.17~222 (2002), 대한재활의학회
- Longo UG., "Acute ruptures of the achilles tendon", Sports Med Arthrosc, 17, pp.127~138 (2009)
- Amiel D., "The effect of immobilization on collagen turnover in connective tissue : a biochemical-biomechanical correlation", Acta Orthop Scand, 53, pp.325~332 (1982)
- Harwood FL., "Differential metabolic responses of periarticular ligaments and tendon to joint immobilization", J Appl Physiol, 72, pp.1687~1691 (1992)
- Savolainen J., "Effects of denervation and immobilization on collagen synthesis in rat skeletal muscle and tendon", Am J Physiol, 254(6 Pt 2), pp.R897~R902 (1988)
- Walsh S., "Immobilization alters cell metabolism in an immature ligament", Clin Orthop Relat Res, (277), pp.277~288 (1992)
- Woo SL., "The biomechanical and morphological changes in the medial collateral ligament of the rabbit after immobilization and remobilization", J Bone Joint Surg Am, 69, pp.1200~1211 (1987)
- Almeida-Silveira MI., "Changes in stiffness induced by hindlimb suspension in rat Achilles tendon", Eur J Appl Physiol, 81, pp.252~257 (2000)
- Loitz BJ., "Effects of short-term immobilization versus continuous passive motion on the biomechanical and biochemical properties of the rabbit tendon", Clin Orthop Relat Res, (244), pp.265~271 (1989)
- Mullner T., "Mechanical properties of a rat patellar tendon stress-shielded in situ", Arch Orthop Trauma Surg, 120, pp.70~74 (2000)
- Trebacz H., "Effect of immobilization in a lengthened position on mechanical properties of the Achilles tendon in growing rats", Acta Bioeng Biomech, 7, pp.79~86 (2005)
- Herbert RD., "The effect of position of immobilisation on resting length, resting stiffness, and weight of the soleus muscle of the rabbit", J Orthop Res, 11, pp.358~366 (1993)
- 김경천, "가토의 아킬레스건 파열의 수술적 치료 후 고정 방법에 따른 생역학적 및 조직학적 변화", 대한정형외과학회지, 40(3), pp.340~346 (2005), 대한정형외과학회
- Dogan A., "Biomechanical comparison of Achilles tenotomy and achilloplasty techniques in young rats : an experimental study", J Am Podiatr Med Assoc, 99, pp.216~222 (2009)
- Schizas N., "Compression therapy promotes proliferative repair during rat Achilles tendon immobilization", J Orthop Res, 28, pp.852~858 (2010)
- Glazebrook MA., "Histological analysis of achilles tendons in an overuse rat model", J Orthop Res, 26, pp.840~846 (2008)
- Murrell GA., "Achilles tendon injuries : a comparison of surgical repair versus no repair in a rat model", Foot Ankle, 14, pp.400~406 (1993)
- Murrell GA., "Effects of immobilization on Achilles tendon healing in a rat model", J Orthop Res, 12, pp.582~591 (1994)
- Best TM., "Achilles tendon healing : a correlation between functional and mechanical performance in the rat", J Orthop Res, 11, pp.897~906 (1993)
- Larsen NP., "Immobilization and retraining of cruciate ligaments in the rat", Acta Orthop Scand, 58, pp.260~264 (1987)
- Bidder M., "Expression of mRNA for vascular endothelial growth factor at the repair site of healing canine flexor tendon", J Orthop Res, 18, pp.247~252 (2000)
- Mass DP., "Effects of constant mechanical tension on the healing of rabbit flexor tendons", Clin Orthop Relat Res, (296), pp.301~306 (1993)
- Liu SH., "Collagen in tendon, ligament, and bone healing: a current review", Clin Orthop Relat Res, (318), pp.265~278 (1995)
- Noyes FR., "Functional properties of knee ligaments and alterations induced by immobilization: a correlative biomechanical and histological study in primates", Clin Orthop Relat Res, (123), pp.210~242 (1977)