HY 항원 불일치 췌도 이식에 의한 면역 관용의 유도
Immune Tolerance in Murine Islet Transplantation Across HY Disparity
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Abstract
Background: Minor histocompatibility HY antigen, as a transplantation antigen, has been known to cause graft rejection in MHC (major histocompatibility complex) matched donor-recipient. The aim of our study is to investigate the role of male antigen (HY) disparity on MHC matched pancreatic islet transplantation and to examine the mechanism of the immune reaction. Methods: Pancreatic islets were isolated and purified by collagen digestion followed by Ficoll gradient. The isolated islets of male C57BL6/J were transplanted underneath the kidney capsule of syngeneic female mice rendered diabetic with streptozotocine. Blood glucose was monitored for the rejection of engrafted islets. After certain period of time, tail to flank skin transplantation was performed either on mouse transplanted with HY mismatched islets or on sham treated mouse. The rejection was monitored by scoring gross pathology of the engrafted skin. Results: HY mismatched islets survived more than 300 days in 14 out of 15 mice. The acceptance of second party graft (male B6 islets) and the rejection of third party graft (male BALB/c islets) in these mice suggested the tolerance to islets with HY disparity. B6 Skin with HY disparity was rejected on day 25 7. However, HY mismatched skin transplanted on the mice tolerated to HY mismatched islets survived more than 240 days. Tetramer staining in these mice indicated the CTL recognizing MHC Db/Uty was not deleted or anergized. Conclusion: The islet transplantation across HY disparity induced tolerance to HY antigen in C57BL6 mouse, which in turn induced tolerance to HY mismatched skin, which otherwise would be rejected within 25 days. The MHC tetramer staining suggested the underlying mechanisms would not be clonal deletion or anergy. (Immune Network 2004;4(1):53-59)
참고문헌 (31)
- "Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen", 343, pp.230~238 (2000)
- "Differential roles of Mac-1 cells and CD4 and CD8 T lymphocytes in primary nonfunction and classic rejection of islet allografts", 172, pp.291~302 (1990)
- "Effect of 15-deoxyspergualin on immediate function and long-term survival of transplanted islets in murine recipients of a marginal islet mass", 43, pp.778~783 (1994)
- "Cytokines induce both necrosis and apoptosis via a common Bcl-2-inhibitable pathway in rat insulin-producing cells", 141, pp.2003~2010 (2000)
- "Islet cell damage associated with tacrolimus and cyclosporine morphological features in pancreas allograft biopsies and clinical correlation", 68, pp.396~402 (1999)
- "Cyclosporine and islet mass--implications for islet transplantation", 54, pp.375~380 (1993)
- "Prevention of immune rejection in rat islet allografts by continuous SC administration of FK 506 using a mini-osmotic pump", 23, pp.1~ (1991)
- "Contributions of direct and indirect T cell alloreactivity during allograft rejection in mice", 162, pp.352~358 (1999)
- "Direct and indirect recognition:the role of MHC antigens in graft rejection: the role of MHC antigens in graft rejection.", 20, pp.77~82 (1999)
- "The basis of immunogenicity of endocrine allografts.", 21, pp.87~101 (2001)
- "Genetics and Immunology of sex- linked antigens.", 15, pp.215~ (1972)
- "Cytotoxic T-cell responses to H-Y:mapping of the Ir genes", 147, pp.758~767 (1978)
- "Minor histocompatibility antigens.", 9, pp.655~661 (1997)
- "The original function of MHC antigens as the general plasma membrane anchorage site of organogenesis- direction proteins.", 33, pp.59~ (1977)
- "Responsiveness to H-Y antigen, Ir gene complementation and target cell specificity.", immu(logical rev 35) (1977)
- "Cytotoxic T-cell responses to H-Y:correlation with the rejection of syngeneic male skin grafts", 147, pp.768~775 (1978)
- "In vitro cell-mediated immune responses to the male specific antigen in mice", pp.1108~1120 (1975)
- "Allelic forms of a gene controlling the female immune response to the male antigen in mice", 11, pp.426~428 (1971)
- "Immune-response gene control of cytotoxic T-cell responses to H-Y", 9, pp.885~888 (1977)
- "THE MALE-SPECIFIC HISTOCOMPATIBILITY ANTIGEN, H-Y: A History of Transplantation, immune Response Genes, Sex determination and Expression Cloning.", 15, pp.39~61 (1997)
- "Genetics and immunology of sex- linked antigens", 15, pp.215~247 (1972)
- "The role of H-Y as a minor transplantation antigen", 3, pp.97~106 (1982)
- "H-Y and sex reversal:minireview", 44, pp.813~814 (1986)
- "Restriction of in vitro mediated cytotoxicity in lymphocytic choriomeningitis within a syngeneic or semi-allogenic system", 248, pp.701~702 (1974)
- "The immunogenomics of minor histocompatibility antigens.", 190, pp.86~94 (2002)
- "Method for the isolation of intact islets of Langerhans from the rat pancreas", 16, pp.35~39 (1967)
- "Survival of H-Y-incompatible endocrine grafts in mice and rats", 31, pp.145~147 (1981)
- "Exocrine contamination impairs implantation of pancreatic islets transplanted beneath the kidney capsule", 45, pp.432~442 (1988)
- "Contribution of nonendocrine tissue to the immunogenicity of endocrine pancreatic transplants", 22, pp.2331~ (1990)
- "'Infectious' transplantation tolerance", 259, pp.974~977 (1993)
- "Induction of 'infectious' tolerance to MHC-incompatible cardiac allografts in CD4 monoclonal antibody-treated sensitized rat recipients.", 157, pp.1944~1950 (1996)