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p38 Mitogen-Activated Protein Kinase and Extracellular Signal-Regulated Kinase Regulate Nitric Oxide Production and Inflammatory Cytokine Expression in Raw Cells

김상현|최철희
대한면역학회 (The Korean Association of Immunologists)|2005.03|Immune Network|제5권 제1호|pp. 30-35

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Abstract

Background: p38 and extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK) signaling are thought to have critical role in lipopolysaccharide (LPS)-induced immune response but the molecular mechanism underlying the induction of these signaling are not clear. Methods: Specific inhibitors for p38, SB203580, and for ERK, PD98059 were used. Cells were stimulated by LPS with or without specific MAPK inhibitors. Results: LPS activated inducible nitric oxide synthase (iNOS), subsequent NO productions, and pro-inflammatory cytokine gene expressions (TNF-α, IL-1β, IL-6, and IL-12). Treatment of both SB203580 and PD98059 decreased LPS- induced NO productions. Concomitant decreases in the expression of iNOS mRNA and protein were detected. SB203580 and PD98059 decreased LPS-induced gene expression of IL-1β and IL-6. SB203580 increased LPS-induced expression of TNF-α and IL-12, and reactive oxygen species production, but PD98059 had no effect. Conclusion: These results indicate that both p38 and ERK pathways are involved in LPS-stimulated NO synthesis, and expression of IL-1β and IL-6. p38 signaling pathways are involved in LPS-induced TNF-α and IL-12, and reactive oxygen species plays an important role in these signaling in macrophage.

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관련 논문AI추천

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