본문 바로가기
KERD
논문KCI 등재

실험적으로 항원에 의하여 일차 자극된 CD4+ T 세포의 이차 면역 반응의 분석

The Kinetics of Secondary Response of Antigen-Specific CD4+ T Cells Primed in vitro with Antigen

박성옥|한영우|윤현아|어성국|Junu A. George|Abi G. Aleyas
대한면역학회 (The Korean Association of Immunologists)|2006.06|Immune Network|제6권 제3호

1

조회

0

다운로드

1

인용

0

참고문헌

초록

Abstract

Background: Memory T lymphocytes of the immune system provide long-term protection in response to bacterial or viral infections/immunization. Ag concentration has also been postulated to be important in determining whether T cell differentiation favors effector versus memory cell development. In the present study we hypothesized that na ve Ag-specific CD4 T cells briefly stimulated with different Ag doses at the primary exposure could affect establishment of memory cell pool after secondary immunization. Methods: To assess this hypothesis, the response kinetics of DO11.10 TCR CD4 T cells primed with different Ag doses in vitro was measured after adoptive transfer to naive BALB/c mice. Results: Maximum expansion was shown in cells primarily stimulated with high doses of ovalbumin peptide (OVA323-339), whereas cells in vitro stimulated with low dose were expanded slightly after in vivo secondary exposure. However, the cells primed with low OVA323-339 peptide dose showed least contraction and established higher number of memory cells than other treated groups. When the cell division was analyzed after adoptive transfer, the high dose Ag-stimulated donor cells have undergone seven rounds of cell division at 3 days post-adoptive transfer. However, there was very few division in naive and low dose of peptide-treated group. Conclusion: These results suggest that primary stimulation with a low dose of Ag leads to better memory CD4 T cell generation after secondary immunization. Therefore, these facts imply that optimally primed CD4 T cells is necessary to support effective memory pool following administration of booster dose in prime-boost vaccination. (Immune Network 2006;6(2):93-101)

참고문헌 (40)

  1. "Immune deficiency, immune silencing, and clonal exhaustion of T cell responses during viral infections", 2, pp.382~387 (1999)
  2. "Costimulation in antiviral immunity", 12, pp.448~455 (2000)
  3. "Effector and memory T-cell differentiation", 2, pp.251~262 (2002)
  4. "Influence of effector molecules on the CD8( ) T cell response to infection", 14, pp.360~365 (2002)
  5. "Virus-specific CD8 T-cell memory determined by clonal burst size", 369, pp.652~654 (1994)
  6. "Role of apoptosis in the regulation of virus-induced T cell responses", pp.135~142 (1995), immune suppression
  7. "Immunological memory and protective immunity", 272, pp.54~60 (1996)
  8. "Programmed contraction of CD8( ) T cells after infection", 3, pp.619~626 (2002)
  9. "Viral persistence alters CD8 T-cell immunodominance and tissue distribution and results in distinct stages of functional impairment", 77, pp.4911~4927 (2003)
  10. "Viral infection results in massive CD8 T cell expansion and mortality in vaccinated perforin-deficient mice", 18, pp.463~474 (2003)
  11. "Immunobiology of dendritic cells", 18, pp.767~811 (2000)
  12. "Co-stimulation and selection for T-cell help for germinal centres", 21, pp.333~337 (2000)
  13. "In vivo activation of antigen- specific CD4 T cells", 19, pp.23~45 (2001)
  14. "Influence of effector molecules on the CD8( ) T cell response to infection", 14, pp.360~365 (2002)
  15. "Differences in maintenance of CD8 and CD4 bacteria-specific effector-memory T cell populations", 33, pp.2875~2885 (2003)
  16. "Differential regulation of antiviral T-cell immunity results in stable CD8 but declining CD4 T-cell memory", 7, pp.913~919 (2001)
  17. "Independent regulation of lymphocytic choriomeningitis virus-specific T cell memory pools", 166, pp.1554~1561 (2001)
  18. "IL-7 promotes the transition of CD4 effectors to persistent memory cells", 198, pp.1807~1815 (2003)
  19. "Interleukin 7 regulates the survival and generation of memory CD4 cells", 198, pp.1797~1806 (2003)
  20. "Interleukin 7 and T cell receptor signals regulate homeostasis of CD4 memory cells", 4, pp.680~686 (2003)
  21. "Interleukin-7 mediates the homeostasis of naive and memory CD8 T cells in vivo", 1, pp.426~432 (2000)
  22. "Interleukin 15 is required for proliferative renewal of virus-specific memory CD8 T cells", 195, pp.1541~1548 (2002)
  23. "Interleukin (IL)-15 and IL-7 jointly regulate homeostatic proliferation of memory phenotype CD8 cells but are not required for memory phenotype CD4 cells", 195, pp.1523~1532 (2002)
  24. "Potent and selective stimulation of memory-phenotype CD8 T cells in vivo by IL-15", 8, pp.591~599 (1998)
  25. "Cytokine requirements for acute and Basal homeostatic proliferation of naive and memory CD8 T cells", 195, pp.1515~1522 (2002)
  26. "Interleukin 15 controls both proliferation and survival of a subset of memory-phenotype CD8( ) T cells", 196, pp.935~946 (2002)
  27. "Following the fate of individual T cells throughout activation and clonal expansion", 100, pp.3173~3183 (1997)
  28. "Early programming of T cell populations responding to bacterial infection", 165, pp.6833~6839 (2000)
  29. "Substantial in vivo proliferation of CD4( ) and CD8( ) T lymphocytes during secondary Listeria monocytogenes infection", 30, pp.1053~1059 (2000)
  30. "In the absence of extrinsic signals, nutrient utilization by lymphocytes is insufficient to maintain either cell size or viability", 6, pp.683~692 (2000)
  31. "Social controls on cell survival and cell death", 356, pp.397~400 (1992)
  32. "Cytokine- induced survival of activated T cells in vitro and in vivo", 95, pp.3810~3815 (1998)
  33. "The duration of antigenic stimulation determines the fate of naive and effector T cells", 8, pp.89~95 (1998)
  34. "The interplay between the duration of TCR and cytokine signaling determines T cell polarization", 29, pp.4092~4101 (1999)
  35. "Two subsets of memory T lymphocytes with distinct homing potentials and effector functions", 401, pp.708~712 (1999)
  36. "Visualizing the generation of memory CD4 T cells in the whole body", 410, pp.101~105 (2001)
  37. "T cell priming by dendritic cells", 32, pp.2046~2054 (2002)
  38. "Duration of infection and antigen display have minimal influence on the kinetics of the CD4 T cell response to Listeria monocytogenes infection", 173, pp.5679~5687 (2004)
  39. "The economy of T-cell memory", 7, pp.892~893 (2001)
  40. "T cell death and memory", 293, pp.245~248 (2001)

이 간행물의 다른 논문 (11건)

p38 Kinase Regulates Nitric Oxide-induced Dedifferentiation and Cyclooxygenase-2 Expression of Articular Chondrocytes김송자, 정선우, 조삼래, 유선미pp. 117-122Ectopic Expression of Caveolin-1 Induces COX-2 Expression in Rabbit Articular Chondrocytes via MAP Kinase PathwaySong-Ja Kimpp. 123-127Apoptosis-Induced Gene Profiles of a Myeloma Cell P3-X63-Ag8.653Hye-Seungn Bahng, Yong-Hoon Chungpp. 128-137덱사메타손이 생쥐 B세포의 세포 표면 인식자와 분화에 미치는 영향차창일, 여승근, 박동춘pp. 138-144종양 세포 용해액에 따른 수지상세포 유도 항원 특이 면역반응 차이의 기전 연구이현아, 손혜진, 김명주, 백소영, 이강은pp. 145-153
원문 보기