Further Characterization of Activin A-induced IgA Response in Murine B Lymphocytes
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We have recently shown that activin A, a member of TGF-β superfamily, stimulates mouse B cells to express IgA isotype but other isotypes. In the present study, we further characterized effects of activin A on B cell growth and IgA expression. We found that activin A did not have effect on LPS-stimulated cell viability. In parallel, CFSE staining analysis revealed that activin A did not alter cell division. An increase of IgA secretion by activin A was completely abrogated by anti-activin A Ab but not by anti-TGFβ1 Ab. In the same conditions, no other isotypes are significantly affected by each antibody treatment. Finally, activin A, as similar to TGF-β1, increased IgA secretion by mesenteric lymph node cells. These results suggest that activin A can specifically stimulate IgA response, independent of TGF-β in the gut.
Abstract
We have recently shown that activin A, a member of TGF-β superfamily, stimulates mouse B cells to express IgA isotype but other isotypes. In the present study, we further characterized effects of activin A on B cell growth and IgA expression. We found that activin A did not have effect on LPS-stimulated cell viability. In parallel, CFSE staining analysis revealed that activin A did not alter cell division. An increase of IgA secretion by activin A was completely abrogated by anti-activin A Ab but not by anti-TGFβ1 Ab. In the same conditions, no other isotypes are significantly affected by each antibody treatment. Finally, activin A, as similar to TGF-β1, increased IgA secretion by mesenteric lymph node cells. These results suggest that activin A can specifically stimulate IgA response, independent of TGF-β in the gut.
참고문헌 (21)
- Mestecky J, "The common mucosal immune system and current strategies for induction of immune responses in external secretions", J Clin Immunol, 7, pp.265~276 (1987)
- van der Heijden PJ, "Contribution of immunoglobulin-secreting cells in the murine small intestine to the total background immunoglobulin production", Immunology, 62, pp.551~555 (1987)
- Fagarasan S, "Intestinal IgA synthesis: regulation of front-line body defences", Nat Rev Immunol, 3, pp.63~72 (2003)
- Kim PH, "Transforming growth factor-beta 1 is a costimulator for IgA production", J Immunol, 144, pp.3411~3416 (1990)
- Sonoda E, "Transforming growth factor beta induces IgA production and acts additively with interleukin 5 for IgA production", J Exp Med, 170, pp.1415~1420 (1989)
- Coffman RL, "Transforming growth factor beta specifically enhances IgA production by lipopolysaccharide-stimulated murine B lymphocytes", J Exp Med, 170, pp.1039~1044 (1989)
- van Vlasselaer P, "Transforming growth factor-beta directs IgA switching in human B cells", J Immunol, 148, pp.2062~2067 (1992)
- Spieker-Polet H, "In vitro induction of the expression of multiple IgA isotype genes in rabbit B cells by TGF-beta and IL-2", J Immunol, 162, pp.5380~5388 (1999)
- McIntyre TM, "Transforming growth factor beta 1 selectivity stimulates immunoglobulin G2b secretion by lipopolysaccharide-activated murine B cells", J Exp Med, 177, pp.1031~1037 (1993)
- Kehrl JH, "Transforming growth factor-beta is a potent negative regulator of human lymphocytes", Ann N Y Acad Sci, 628, pp.345~353 (1991)
- Smeland EB, "Transforming growth factor type beta (TGF beta) inhibits G1 to S transition, but not activation of human B lymphocytes", Exp Cell Res, 171, pp.213~222 (1987)
- Cross D, "Transforming growth factor beta 1 has differential effects on B cell proliferation and activation antigen expression", J Immunol, 144, pp.432~439 (1990)
- Luisi S, "Expression and secretion of activin A: possible physiological and clinical implications", Eur J Endocrinol, 145, pp.225~236 (2001)
- Ogawa K, "Activin A functions as a Th2 cytokine in the promotion of the alternative activation of macrophages", J Immunol, 177, pp.6787~6794 (2006)
- Lee HJ, "Activin A stimulates IgA expression in mouse B cells", Biochem Biophys Res Commun, 366, pp.574~578 (2008)
- Nakamura M, "High frequency class switching of an IgM+ B lymphoma clone CH12F3 to IgA+ cells", Int Immunol, 8, pp.193~201 (1996)
- Park SR, "Smad3 and Smad4 mediate transforming growth factor-beta1-induced IgA expression in murine B lymphocytes", Eur J Immunol, 31, pp.1706~1715 (2001)
- Kim PH, "Transforming growth factor beta 1 increases IgA isotype switching at the clonal level", J Immunol, 145, pp.3773~3778 (1990)
- Craig SW, "Peyer's patches: an enriched source of precursors for IgA-producing immunocytes in the rabbit", J Exp Med, 134, pp.188~200 (1971)
- Santiago AF, "Role of mesenteric lymph nodes and aging in secretory IgA production in mice", Cellular immunology, 253, pp.5~10 (2008)
- Kawanishi H, "Mechanisms regulating IgA class-specific immunoglobulin production in murine gut-associated lymphoid tissues. II. Terminal differentiation of postswitch sIgA-bearing Peyer's patch B cells", J Exp Med, 158, pp.649~669 (1983)