Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen
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Background: Disparities of Minor H antigens can induce graft rejection after MHC-matched transplantation. H60 has been characterized as a dominant antigen expressed on hematopoietic cells and considered to be an ideal model antigen for study on graft-versus-leukemia effect. Methods: Splenocytes from C57BL/6 mice immunized with H60 congenic splenocytes were used for establishment of H60-specific CTL clones. Then the clones were characterized for proliferation capacity and cytotoxicity after stimulation with H60. Clone #14, #15, and #23 were tested for the TCR binding avidity to H60-peptide/H-2Kb and analyzed for TCR sequences. Results: H60-specific CTL clones showed different levels of proliferation capacity and cytotoxic activity to H60-stimulation. Clones #14, #15, and #23 showed high proliferation activity, high cytotoxicity, and low activities on both aspects, respectively, and have TCRs with different binding avidities to H60-peptide/H-2Kb with t1/2 values of 4.87, 6.92, and 13.03 minutes, respectively. The TCR usages were Vα12D-3-01＋Jα11-01 and Vβ12-1-01＋Dβ1-01 ＋J2-7-01 for clone #14, Vα13D-1-02＋Jα34-02 and Vβ13-1-02＋Dβ2-01＋Jβ2-7-01 for clone #15, and Vα16D＋Jα45-01 and Vβ12-1-01＋Dβ1-01＋Jβ2-5-01 for clone #23. Conclusion: The results will be useful for modeling GVL and generation TCR transgenic mouse.
Abstract
Background: Disparities of Minor H antigens can induce graft rejection after MHC-matched transplantation. H60 has been characterized as a dominant antigen expressed on hematopoietic cells and considered to be an ideal model antigen for study on graft-versus-leukemia effect. Methods: Splenocytes from C57BL/6 mice immunized with H60 congenic splenocytes were used for establishment of H60-specific CTL clones. Then the clones were characterized for proliferation capacity and cytotoxicity after stimulation with H60. Clone #14, #15, and #23 were tested for the TCR binding avidity to H60-peptide/H-2Kb and analyzed for TCR sequences. Results: H60-specific CTL clones showed different levels of proliferation capacity and cytotoxic activity to H60-stimulation. Clones #14, #15, and #23 showed high proliferation activity, high cytotoxicity, and low activities on both aspects, respectively, and have TCRs with different binding avidities to H60-peptide/H-2Kb with t1/2 values of 4.87, 6.92, and 13.03 minutes, respectively. The TCR usages were Vα12D-3-01＋Jα11-01 and Vβ12-1-01＋Dβ1-01 ＋J2-7-01 for clone #14, Vα13D-1-02＋Jα34-02 and Vβ13-1-02＋Dβ2-01＋Jβ2-7-01 for clone #15, and Vα16D＋Jα45-01 and Vβ12-1-01＋Dβ1-01＋Jβ2-5-01 for clone #23. Conclusion: The results will be useful for modeling GVL and generation TCR transgenic mouse.
참고문헌 (12)
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