Altered Gut Microbiota Composition in Rag1-deficient Mice Contributes to Modulating Homeostasis of Hematopoietic Stem and Progenitor Cells
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Hematopoietic stem and progenitor cells (HSPCs) can produce all kind of blood lineage cells, and gut microbiota that consists of various species of microbe affects development and maturation of the host immune system including gut lymphoid cells and tissues. However, the effect of altered gut microbiota composition on homeostasis of HSPCs remains unclear. Here we show that compositional change of gut microbiota affects homeostasis of HSPCs using Rag1−/− mice which represent lymphopenic condition. The number and proportions of HSPCs in Rag1−/− mice are lower compared to those of wild types. However, the number and proportions of HSPCs in Rag1−/− mice are restored as the level of wild types through alteration of gut microbiota diversity via transferring feces from wild types. Gut microbiota composition of Rag1−/− mice treated with feces from wild types shows larger proportions of family Prevotellaceae and Helicobacterceae whereas lower proportions of family Lachnospiraceae compared to unmanipulated Rag1−/− mice. In conclusion, gut microbiota composition of lymphopenic Rag1−/− mice is different to that of wild type, which may lead to altered homeostasis of HSPCs.
Abstract
Hematopoietic stem and progenitor cells (HSPCs) can produce all kind of blood lineage cells, and gut microbiota that consists of various species of microbe affects development and maturation of the host immune system including gut lymphoid cells and tissues. However, the effect of altered gut microbiota composition on homeostasis of HSPCs remains unclear. Here we show that compositional change of gut microbiota affects homeostasis of HSPCs using Rag1−/− mice which represent lymphopenic condition. The number and proportions of HSPCs in Rag1−/− mice are lower compared to those of wild types. However, the number and proportions of HSPCs in Rag1−/− mice are restored as the level of wild types through alteration of gut microbiota diversity via transferring feces from wild types. Gut microbiota composition of Rag1−/− mice treated with feces from wild types shows larger proportions of family Prevotellaceae and Helicobacterceae whereas lower proportions of family Lachnospiraceae compared to unmanipulated Rag1−/− mice. In conclusion, gut microbiota composition of lymphopenic Rag1−/− mice is different to that of wild type, which may lead to altered homeostasis of HSPCs.
참고문헌 (18)
- Kondo, M., "Biology of hematopoietic stem cells progenitors: implications for clinical application", Annu. Rev. Immunol, 21, pp.759~806 (2003)
- Warr, M. R., "Mechanisms controlling hematopoietic stem cell functions during normal hematopoiesis hematological malignancies", Wiley Interdiscip. Rev. Syst. Biol. Med, 3, pp.681~701 (2011)
- van der Wath, R. C., "Estimating dormant active hematopoietic stem cell kinetics through extensive modeling of bromodeoxyuridine label-retaining cell dynamics", PLoS One, pp.e6972~ (2009)
- Trumpp, A., "Awakening dormant haematopoietic stem cells", Nat. Rev. Immunol, 10, pp.201~209 (2010)
- Wilson, A., "Bone-marrow haematopoietic-stem-cell niches", Nat. Rev. Immunol, 6, pp.93~106 (2006)
- Arai, F., "Niche regulation of hematopoietic stem cells in the endosteum", Ann. N.Y. Acad. Sci, 1176, pp.36~46 (2009)
- Kiel, M. J., "Uncertainty in the niches that maintain haematopoietic stem cells", Nat. Rev. Immunol, 8, pp.290~301 (2008)
- Busch, K., "Fundamental properties of unperturbed haematopoiesis from stem cells in vivo", Nature, 518, pp.542~546 (2015)
- Wilson, A., "Hematopoietic stem cells reversibly switch from dormancy to self-renewal during homeostasis repair", Cell, 135, pp.1118~1129 (2008)
- Sommer, F., "The gut microbiota--masters of host development physiology", Nat. Rev. Microbiol, 11, pp.227~238 (2013)
- Joshua Lederberg, "Alexa Mccray. 'Ome Sweet 'Omics--A Genealogical Treasury of Words", The Scientist, 17(7) (2001)
- NIH HMP Working Group, "The NIH Human Microbiome Project", Genome Res, 19, pp.2317~2323 (2009)
- Marsland, B. J., "Host-microorganism interactions in lung diseases", Nat. Rev. Immunol, 14, pp.827~835 (2014)
- Park, S. J., "Negative role of inducible PD-1 on survival of activated dendritic cells", J. Leukoc. Biol, 95, pp.621~629 (2014)
- Khosravi, A., "Gut microbiota promote hematopoiesis to control bacterial infection", Cell Host Microbe, 15, pp.374~381 (2014)
- Zhang, H., "Host adaptive immunity alters gut microbiota", ISME J, 9, pp.770~781 (2015)
- Scholz, F., "Immune mediated shaping of microflora community composition depends on barrier site", PLoS One, 9, pp.e84019~ (2014)
- Antonopoulos, D. A., "Reproducible community dynamics of the gastrointestinal microbiota following antibiotic perturbation", Infect. Immun, 77, pp.2367~2375 (2009)