SARS-CoV-2 Infection of Airway Epithelial Cells
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Coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been spreading worldwide since its outbreak in December 2019, and World Health Organization declared it as a pandemic on March 11, 2020. SARS-CoV-2 is highly contagious and is transmitted through airway epithelial cells as the first gateway. SARS-CoV-2 is detected by nasopharyngeal or oropharyngeal swab samples, and the viral load is significantly high in the upper respiratory tract. The host cellular receptors in airway epithelial cells, including angiotensin-converting enzyme 2 and transmembrane serine protease 2, have been identified by single-cell RNA sequencing or immunostaining. The expression levels of these molecules vary by type, function, and location of airway epithelial cells, such as ciliated cells, secretory cells, olfactory epithelial cells, and alveolar epithelial cells, as well as differ from host to host depending on age, sex, or comorbid diseases. Infected airway epithelial cells by SARS-CoV-2 in ex vivo experiments produce chemokines and cytokines to recruit inflammatory cells to target organs. Same as other viral infections, IFN signaling is a critical pathway for host defense. Various studies are underway to confirm the pathophysiological mechanisms of SARS-CoV-2 infection. Herein, we review cellular entry, host-viral interactions, immune responses to SARS-CoV-2 in airway epithelial cells. We also discuss therapeutic options related to epithelial immune reactions to SARS-CoV-2.
Abstract
Coronavirus disease 2019 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been spreading worldwide since its outbreak in December 2019, and World Health Organization declared it as a pandemic on March 11, 2020. SARS-CoV-2 is highly contagious and is transmitted through airway epithelial cells as the first gateway. SARS-CoV-2 is detected by nasopharyngeal or oropharyngeal swab samples, and the viral load is significantly high in the upper respiratory tract. The host cellular receptors in airway epithelial cells, including angiotensin-converting enzyme 2 and transmembrane serine protease 2, have been identified by single-cell RNA sequencing or immunostaining. The expression levels of these molecules vary by type, function, and location of airway epithelial cells, such as ciliated cells, secretory cells, olfactory epithelial cells, and alveolar epithelial cells, as well as differ from host to host depending on age, sex, or comorbid diseases. Infected airway epithelial cells by SARS-CoV-2 in ex vivo experiments produce chemokines and cytokines to recruit inflammatory cells to target organs. Same as other viral infections, IFN signaling is a critical pathway for host defense. Various studies are underway to confirm the pathophysiological mechanisms of SARS-CoV-2 infection. Herein, we review cellular entry, host-viral interactions, immune responses to SARS-CoV-2 in airway epithelial cells. We also discuss therapeutic options related to epithelial immune reactions to SARS-CoV-2.
참고문헌 (112)
- Wang D, "Clinical characteristics of 138 hospitalized patients with 2019 novel coronavirus-infected pneumonia in Wuhan, China", JAMA, 323, pp.1061~1069 (2020)
- Zhu N, "A novel coronavirus from patients with pneumonia in China, 2019", N Engl J Med, 382, pp.727~733 (2020)
- Wu F, "A new coronavirus associated with human respiratory disease in China", Nature, 579, pp.265~269 (2020)
- Zhou P, "A pneumonia outbreak associated with a new coronavirus of probable bat origin", Nature, 579, pp.270~273 (2020)
- Larsen JR, "Modeling the onset of symptoms of COVID-19", Front Public Health, 8, pp.473~ (2020)
- Riphagen S, "Hyperinflammatory shock in children during COVID-19 pandemic", Lancet, 395, pp.1607~1608 (2020)
- Richard M, "SARS-CoV-2 is transmitted via contact and via the air between ferrets", Nat Commun, 11, pp.3496~ (2020)
- Zhang R, "Identifying airborne transmission as the dominant route for the spread of COVID-19", Proc Natl Acad Sci U S A, 117, pp.14857~14863 (2020)
- Zou L, "SARS-CoV-2 viral load in upper respiratory specimens of infected patients", N Engl J Med, 382, pp.1177~1179 (2020)
- Vlek ALM, "Combined throat", nasal swab sampling for SARSCoV-2 is equivalent to nasopharyngeal sampling, 2021, pp.40, 193~195
- Wang H, "Nasopharyngeal swabs are more sensitive than oropharyngeal swabs for COVID-19 diagnosis and monitoring the SARS-CoV-2 load", Front Med(Lausanne), 7, pp.334~ (2020)
- Sungnak W, "SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes", Nat Med, 26, pp.681~687 (2020)
- Monica Fung, "Zoonotic coronavirus epidemics", Annals of Allergy, Asthma & Immunology, 126(4), pp.321~337 (2021), Elsevier BV[DOI]
- Peiris JS, "The severe acute respiratory syndrome", N Engl J Med, 349, pp.2431~2441 (2003)
- Li W, "Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus", Nature, 426, pp.450~454 (2003)
- Hoffmann M, "SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor", Cell, 181, pp.271~280 (2020)
- Bertram S, "Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts", PLoS One, 7, pp.e35876~ (2012)
- Du M, "Multiomics evaluation of gastrointestinal and other clinical characteristics of COVID-19", Gastroenterology, 158, pp.2298.e7~2301.e7 (2020)
- Wrapp D, "Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation", Science, 367, pp.1260~1263 (2020)
- Oreste Gallo, "The central role of the nasal microenvironment in the transmission, modulation, and clinical progression of SARS-CoV-2 infection", Mucosal Immunology, 14(2), pp.305~316 (2021), Springer Science and Business Media LLC[DOI]
- Bedford J, "COVID-19 : towards controlling of a pandemic", Lancet, 395, pp.1015~1018 (2020)
- Gordon DE, "A SARS-CoV-2 protein interaction map reveals targets for drug repurposing", Nature, 583, pp.459~468 (2020)
- Patel VB, "Role of the ACE2", angiotensin 1–7 axis of the renin-angiotensin system in heart failure, 2016, pp.118, 1313~1326
- Lee IT, "ACE2 localizes to the respiratory cilia and is not increased by ACE inhibitors or ARBs", Nat Commun, 11, pp.5453~ (2020)
- Imai Y, "Angiotensinconverting enzyme 2 protects from severe acute lung failure", Nature, 436, pp.112~116 (2005)
- Kuba K, "A crucial role of angiotensin converting enzyme 2(ACE2)in SARS coronavirus-induced lung injury", Nat Med, 11, pp.875~879 (2005)
- Huang F, "Angiotensin II plasma levels are linked to disease severity and predict fatal outcomes in H7N9-infected patients", Nat Commun, 5, pp.3595~ (2014)
- Yan R, "Structural basis for the recognition of SARS-CoV-2 by fulllength human ACE2", Science, 367, pp.1444~1448 (2020)
- Raj VS, "Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC", Nature, 495, pp.251~254 (2013)
- Ziegler CGK, "SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues", Cell, 181, pp.1016~1035 (2020)
- Tata PR, "Dedifferentiation of committed epithelial cells into stem cells in vivo", Nature, 503, pp.218~223 (2013)
- Chen M, "Elevated ACE-2 expression in the olfactory neuroepithelium : implications for anosmia and upper respiratory SARS-CoV-2 entry and replication", Eur Respir J, 56, pp.2001948~ (2020)
- Chua RL, "COVID-19 severity correlates with airway epithelium-immune cell interactions identified by single-cell analysis", Nat Biotechnol, 38, pp.970~979 (2020)
- Coutard B, "The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade", Antiviral Res, 176, pp.104742~ (2020)
- Lukassen S, "SARS-CoV-2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells", EMBO J, 39, pp.e105114~ (2020)
- Aguiar JA, "Gene expression and in situ protein profiling of candidate SARS-CoV-2receptors in human airway epithelial cells and lung tissue", Eur Respir J, 56, pp.2001123~ (2020)
- Cantuti-Castelvetri L, "Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity", Science, 370, pp.856~860 (2020)
- Daly JL, "Neuropilin-1 is a host factor for SARS-CoV-2 infection", Science, 370, pp.861~865 (2020)
- Drożdżal S, "FDA approved drugs with pharmacotherapeutic potential for SARS-CoV-2(COVID-19)therapy", Drug Resist Updat, 53, pp.100719~ (2020)
- Milewska A, "Replication of severe acute respiratory syndrome coronavirus 2 in human respiratory epithelium", J Virol, 94, pp.e00957~20 (2020)
- Sallenave JM, "Innate immune signaling and proteolytic pathways in the resolution or exacerbation of SARS-CoV-2 in COVID-19 : key therapeutic targets", Front Immunol, 11, pp.1229~ (2020)
- Fiege JK, "Single cell resolution of SARS-CoV-2 tropism, antiviral responses, and susceptibility to therapies in primary human airway epithelium", PLoS Pathog, 17, pp.e1009292~ (2021)
- Hamming I, "Tissue distribution of ACE2 protein, the functional receptor for SARS coronavirus. A first step in understanding SARS pathogenesis", J Pathol, 203, pp.631~637 (2004)
- Harcourt J, "Severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, united states", Emerg Infect Dis, 26, pp.1266~1273 (2020)
- Blanco-Melo D, "Imbalanced host response to SARS-CoV-2 drives development of COVID-19", Cell, 181, pp.1036~1045 (2020)
- Gamage AM, "Infection of human nasal epithelial cells with SARS-CoV-2 and a 382-nt deletion isolate lacking ORF8 reveals similar viral kinetics and host transcriptional profiles", PLoS Pathog, 16, pp.e1009130~ (2020)
- Zhu N, "Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells", Nat Commun, 11, pp.3910~ (2020)
- Hao S, "Long-term modeling of SARS-CoV-2 infection of in vitro cultured polarized human airway epithelium", MBio, 11, pp.e02852~20 (2020)
- Ehre C, "SARS-CoV-2 infection of airway cells", N Engl J Med, 383, pp.969~ (2020)
- Ravindra NG, "Single-cell longitudinal analysis of SARS-CoV-2 infection in human bronchial epithelial cells", bioRxiv, ~ (2020)
- Xie X, "An infectious cDNA clone of SARS-CoV-2", Cell Host Microbe, 27, pp.841~848 (2020)
- Lamers MM, "An organoid-derived bronchioalveolar model for SARS-CoV-2 infection of human alveolar type II-like cells", EMBO J (2020)[DOI]
- Lamers MM, "SARS-CoV-2 productively infects human gut enterocytes", Science, 369, pp.50~54 (2020)
- Salahudeen AA, "Progenitor identification and SARS-CoV-2 infection in human distal lung organoids", Nature, 588, pp.670~675 (2020)
- Han Y, "Identification of SARS-CoV-2 inhibitors using lung and colonic organoids", Nature, 589, pp.270~275 (2021)
- Mulay A, "SARS-CoV-2 infection of primary human lung epithelium for COVID-19 modeling and drug discovery", bioRxiv, ~ (2020)
- Matheson NJ, "How does SARS-CoV-2 cause COVID-19", Science, 369, pp.510~511 (2020)
- Yao Y, "Expression of ACE2 in airways : Implication for COVID-19 risk and disease management in patients with chronic inflammatory respiratory diseases", Clin Exp Allergy, 50, pp.1313~1324 (2020)
- Saheb Sharif-Askari N, "Airways expression of SARS-CoV-2 receptor, ACE2, and TMPRSS2 is lower in children than adults and increases with smoking and COPD", Mol Ther Methods Clin Dev, 18, pp.1~6 (2020)
- Bunyavanich S, "Nasal gene expression of angiotensin-converting enzyme 2 in children and adults", JAMA, 323, pp.2427~2429 (2020)
- Schouten LR, "Age-dependent differences in pulmonary host responses in ARDS : a prospective observational cohort study", Ann Intensive Care, 9, pp.55~ (2019)
- Ortiz ME, "Heterogeneous expression of the SARS-coronavirus-2 receptor ACE2 in the human respiratory tract", EBioMedicine, 60, pp.102976~ (2020)
- Brodin P, "Immune determinants of COVID-19 disease presentation and severity", Nat Med, 27, pp.28~33 (2021)
- Bajaj V, "Aging, immunity, and COVID-19 : how age influences the host immune response to coronavirus infections", Front Physiol, 11, pp.571416~ (2021)
- Inde Z, "Agedependent regulation of SARS-CoV-2 cell entry genes and cell death programs correlates with COVID-19disease severity", bioRxiv 2020, ~ (2020)
- Meftahi GH, "The possible pathophysiology mechanism of cytokine storm in elderly adults with COVID-19 infection : the contribution of"inflame-aging"", Inflamm Res, 69, pp.825~839 (2020)
- Donoghue M, "A novel angiotensin-converting enzyme-related carboxypeptidase(ACE2)converts angiotensin I to angiotensin 1-9", Circ Res, 87, pp.E1~E9 (2000)
- Crackower MA, "Angiotensin-converting enzyme 2 is an essential regulator of heart function", Nature, 417, pp.822~828 (2002)
- Hamming I, "The emerging role of ACE2 in physiology and disease", J Pathol, 212, pp.1~11 (2007)
- Zhang H, "Expression of the SARS-CoV-2 ACE2 receptor in the human airway epithelium", Am J Respir Crit Care Med, 202, pp.219~229 (2020)
- Leung JM, "ACE-2expression in the small airway epithelia of smokers and COPD patients : implications for COVID-19", Eur Respir J, 55, pp.55~ (2020)
- Purkayastha A, "Direct exposure to SARS-CoV-2 and cigarette smoke increases infection severity and alters the stem cell-derived airway repair response", Cell Stem Cell, 27, pp.869.e4~875.e4 (2020)
- Kimura H, "Type 2inflammation modulates ACE2 and TMPRSS2 in airway epithelial cells", J Allergy Clin Immunol, 146, pp.80.e8~88.e8 (2020)
- Sajuthi SP, "Type 2 and interferon inflammation regulate SARS-CoV-2 entry factor expression in the airway epithelium", Nat Commun, 11, pp.5139~ (2020)
- Jackson DJ, "Association of respiratory allergy, asthma, and expression of the SARS-CoV-2receptor ACE2", J Allergy Clin Immunol, 146, pp.203.e3~206.e3 (2020)
- Wang M, "Inflammatory endotypes of CRSwNP and responses to COVID-19", Curr Opin Allergy Clin Immunol, 21, pp.8~15 (2021)
- Hai Wang, "Regional differences in ACE2 expression in the sinonasal mucosa of adult Chinese patients with chronic rhinosinusitis", Allergy, 76(5), pp.1565~1568 (2021), Wiley[DOI]
- Ming Wang, "Distinct expression of SARS‐CoV‐2 receptor ACE2 correlates with endotypes of chronic rhinosinusitis with nasal polyps", Allergy, 76(3), pp.789~803 (2021), Wiley[DOI]
- Narasaraju T, "Histopathologic changes and SARS-CoV-2 immunostaining in the lung of a patient with COVID-19", Ann Intern Med, 173, pp.323~324 (2020)
- Chen IY, "Severe acute respiratory syndrome coronavirus viroporin 3a activates the nlrp3 inflammasome", Front Microbiol, 10, pp.50~ (2019)
- Fink SL, "Apoptosis, pyroptosis, and necrosis : mechanistic description of dead and dying eukaryotic cells", Infect Immun, 73, pp.1907~1916 (2005)
- Tay MZ, "The trinity of COVID-19 : immunity, inflammation and intervention", Nat Rev Immunol, 20, pp.363~374 (2020)
- Lazear HM, "Shared and distinct functions of type i and type iii interferons", Immunity, 50, pp.907~923 (2019)
- Hur S, "Double-stranded RNA sensors and modulators in innate immunity", Annu Rev Immunol, 37, pp.349~375 (2019)
- Sokol CL, "The chemokine system in innate immunity", Cold Spring Harb Perspect Biol, 7, pp.a016303~ (2015)
- Hadjadj J, "Impaired type I interferon activity and inflammatory responses in severe COVID-19patients", Science, 369, pp.718~724 (2020)
- Arunachalam PS, "Systems biological assessment of immunity to mild versus severe COVID-19 infection in humans", Science, 369, pp.1210~1220 (2020)
- Lucas C, "Longitudinal analyses reveal immunological misfiring in severe COVID-19", Nature, 584, pp.463~469 (2020)
- Moore JB, "Cytokine release syndrome in severe COVID-19", Science, 368, pp.473~474 (2020)
- Sa Ribero M, "Interplay between SARS-CoV-2 and the type I interferon response", PLoS Pathog, 16, pp.e1008737~ (2020)
- Bost P, "Host-viral infection maps reveal signatures of severe COVID-19 patients", Cell, 181, pp.1475.e12~1488.e12 (2020)
- Liao M, "Single-cell landscape of bronchoalveolar immune cells in patients with COVID-19", Nat Med, 26, pp.842~844 (2020)
- Lee JS, "Immunophenotyping of COVID-19 and influenza highlights the role of type I interferons in development of severe COVID-19", Sci Immunol, 5, pp.eabd1554~ (2020)
- Chen G, "Clinical and immunological features of severe and moderate coronavirus disease 2019", J Clin Invest, 130, pp.2620~2629 (2020)
- Hirano T, "COVID-19 : a new virus, but a familiar receptor and cytokine release syndrome", Immunity, 52, pp.731~733 (2020)
- Zhuang MW, "Increasing host cellular receptorangiotensin-converting enzyme 2 expression by coronavirus may facilitate 2019-nCoV(or SARS-CoV-2)infection", J Med Virol, 92, pp.2693~2701 (2020)
- Onabajo OO, "Interferons and viruses induce a novel truncated ACE2 isoform and not the fulllength SARS-CoV-2 receptor", Nat Genet, 52, pp.1283~1293 (2020)
- Zhang H, "Angiotensin-converting enzyme 2(ACE2)as a SARSCoV-2 receptor : molecular mechanisms and potential therapeutic target", Intensive Care Med, 46, pp.586~590 (2020)
- Monteil V, "Inhibition of SARS-CoV-2 infections in engineered human tissues using clinicalgrade soluble human ACE2", Cell, 181, pp.905.e7~913.e7 (2020)
- Khan A, "A pilot clinical trial of recombinant human angiotensin-converting enzyme 2 in acute respiratory distress syndrome", Crit Care, 21, pp.234~ (2017)
- Zhang H, "Recombinant human ACE2 : acing out angiotensin II in ARDS therapy", Crit Care, 21, pp.305~ (2017)
- Wang K, "CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells", Signal Transduct Target Ther, 5, pp.283~ (2020)
- Bian H, "Meplazumab treats COVID-19 pneumonia: an open-labelled, concurrent controlled add-on clinical trial", medRxiv 2020 doi: 10.1101, 2020 (2020)
- Lau SKP, "Delayed induction of proinflammatory cytokines and suppression of innate antiviral response by the novel Middle East respiratory syndrome coronavirus : implications for pathogenesis and treatment", J Gen Virol, 94, pp.2679~2690 (2013)
- Cameron MJ, "Interferon-mediated immunopathological events are associated with atypical innate and adaptive immune responses in patients with severe acute respiratory syndrome", J Virol, 81, pp.8692~8706 (2007)
- Felgenhauer U, "Inhibition of SARS-CoV-2 by type I and type III interferons", J Biol Chem, 295, pp.13958~13964 (2020)
- Lokugamage KG, "Type I interferon susceptibility distinguishes SARS-CoV-2 from SARS-CoV", J Virol, 94, pp.e01410~e01420 (2020)
- Mantlo E, "Antiviral activities of type I interferons to SARS-CoV-2 infection", Antiviral Res, 179, pp.104811~ (2020)
- Andreakos E, "Interferon-λs : front-line guardians of immunity and homeostasis in the respiratory tract", Front Immunol, 8, pp.1232~ (2017)
- Broggi A, "Type III interferons : balancing tissue tolerance and resistance to pathogen invasion", J Exp Med, 217, pp.217~ (2020)
- Prokunina-Olsson L, "COVID-19 and emerging viral infections : the case for interferon lambda", J Exp Med, 217, pp.217~ (2020)
- Broggi A, "Type III interferons disrupt the lung epithelial barrier upon viral recognition", Science, 369, pp.706~712 (2020)
