Examination of lysine requirement of healthy young male adults on a Chinese habitual diet by the modified indicator amino acid oxidation method
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There is currently no reference for intake of lysine for Chinese people; therefore, the present study was conducted to determine the lysine requirementof Chinese young male adults on a habitual Chinese mixed diet based on the modified indicator amino acid oxidation method. Seven young menwith a mean age of 23.7 ± 2.2 years that were healthy based on questionnaire, physical examinations and screening tests were evaluated. Subjectswere evaluated over five consecutive 7 day periods, during which time they were administered decreasing amounts of lysine via the diet (65, 55,45, 35, 25 mg·kg-1·d-1). Subjects were allowed to adapt from day 1 to 6 and the isotopes were measured on day 7 in each period. The subjects'body weights, body compositions and plasma proteins were also examined during the study. Amino acid kinetics were measured based on the indicatoramino acid oxidation technique using the 13CO2 release rate and phenylalanine oxidation rate to estimate lysine requirements. Body weights, bodycompositions, and plasma proteins of subjects did not change significantly relative to those at baseline. The mean and the upper 95% CI of lysinerequirements of Chinese habitual diets were determined to be 58.41 and 70.09 mg·kg-1·d-1, respectively, based on the 13CO2 release rate and 54.28and 65.14 mg·kg-1·d-1, respectively, based on the phenylalanine oxidation rate.
Abstract
There is currently no reference for intake of lysine for Chinese people; therefore, the present study was conducted to determine the lysine requirementof Chinese young male adults on a habitual Chinese mixed diet based on the modified indicator amino acid oxidation method. Seven young menwith a mean age of 23.7 ± 2.2 years that were healthy based on questionnaire, physical examinations and screening tests were evaluated. Subjectswere evaluated over five consecutive 7 day periods, during which time they were administered decreasing amounts of lysine via the diet (65, 55,45, 35, 25 mg·kg-1·d-1). Subjects were allowed to adapt from day 1 to 6 and the isotopes were measured on day 7 in each period. The subjects'body weights, body compositions and plasma proteins were also examined during the study. Amino acid kinetics were measured based on the indicatoramino acid oxidation technique using the 13CO2 release rate and phenylalanine oxidation rate to estimate lysine requirements. Body weights, bodycompositions, and plasma proteins of subjects did not change significantly relative to those at baseline. The mean and the upper 95% CI of lysinerequirements of Chinese habitual diets were determined to be 58.41 and 70.09 mg·kg-1·d-1, respectively, based on the 13CO2 release rate and 54.28and 65.14 mg·kg-1·d-1, respectively, based on the phenylalanine oxidation rate.
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