Effect of probiotics Lactobacillus paracasei GKS6, L. plantarum GKM3, and L. rhamnosus GKLC1 on alleviating alcohol-induced alcoholic liver disease in a mouse model
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BACKGROUND/OBJECTIVES: Heavy alcohol consumption causes the development of alcoholic liver disease (ALD), a neglected but important public health problem. Many studies have pointed out that probiotics could improve gut health, which is also considered to be a cause of ALD. Therefore, this study screened the probiotics, Lactobacillus casei GKC1 (GKC1), L. fermentum GKF3 (GKF3), Bifidobacterium lactis GKK2 (GKK2), L. rhamnosus GKLC1 (GKLC1), L. paracasei GKS6 (GKS6), and L. plantarum GKM3 (GKM3), for their potential benefits in alleviating ALD for applications to disease prevention. SUBJECTS/METHODS: C57BL/6N mice were divided into 8 groups (n = 6 in each): normal control, positive control (alcohol-diet fed), and treatments of feeding probiotics GKC1, GKF3, GKK2, GKLC1, GKS6, and GKM3 under an oral dose 0.82 g/kg B.W. per day by oral gavage. The experiment was conducted for 8 weeks, and the concentrations of alanine aminotransferase (ALT), aspartate aminotransferase, triglyceride (TG), and total cholesterol (TC) in mice were measured. The glutathione (GSH), catalase (CAT), and histology were analyzed after sacrifice. RESULTS: The results showed a decrease in the serum ALT, liver TG, and liver TC levels in the GKS6, GKM3, and GKLC1 groups compared to the positive control. In addition, the decreasing GSH and CAT levels were inhibited in the GKS6 and GKM3 groups. The histopathological results showed that all probiotics could reduce the accumulation of liver fat. Furthermore, there was a significant difference in GKLC1 with lower stomach damage compared to the alcohol-fed mice without any addition of probiotics. CONCLUSIONS: GKLC1, GKS6, and GKM3 can be used as supplements for alleviating the development of ALD.
Abstract
BACKGROUND/OBJECTIVES: Heavy alcohol consumption causes the development of alcoholic liver disease (ALD), a neglected but important public health problem. Many studies have pointed out that probiotics could improve gut health, which is also considered to be a cause of ALD. Therefore, this study screened the probiotics, Lactobacillus casei GKC1 (GKC1), L. fermentum GKF3 (GKF3), Bifidobacterium lactis GKK2 (GKK2), L. rhamnosus GKLC1 (GKLC1), L. paracasei GKS6 (GKS6), and L. plantarum GKM3 (GKM3), for their potential benefits in alleviating ALD for applications to disease prevention. SUBJECTS/METHODS: C57BL/6N mice were divided into 8 groups (n = 6 in each): normal control, positive control (alcohol-diet fed), and treatments of feeding probiotics GKC1, GKF3, GKK2, GKLC1, GKS6, and GKM3 under an oral dose 0.82 g/kg B.W. per day by oral gavage. The experiment was conducted for 8 weeks, and the concentrations of alanine aminotransferase (ALT), aspartate aminotransferase, triglyceride (TG), and total cholesterol (TC) in mice were measured. The glutathione (GSH), catalase (CAT), and histology were analyzed after sacrifice. RESULTS: The results showed a decrease in the serum ALT, liver TG, and liver TC levels in the GKS6, GKM3, and GKLC1 groups compared to the positive control. In addition, the decreasing GSH and CAT levels were inhibited in the GKS6 and GKM3 groups. The histopathological results showed that all probiotics could reduce the accumulation of liver fat. Furthermore, there was a significant difference in GKLC1 with lower stomach damage compared to the alcohol-fed mice without any addition of probiotics. CONCLUSIONS: GKLC1, GKS6, and GKM3 can be used as supplements for alleviating the development of ALD.
참고문헌 (37)
- Dey A, "Alcohol and oxidative liver injury", Hepatology, 43, pp.S63~74 (2006)
- Nagata K, "Common pathogenic mechanism in development progression of liver injury caused by non-alcoholic or alcoholic steatohepatitis", J Toxicol Sci, 32, pp.453~468 (2007)
- Ceni E, "Pathogenesis of alcoholic liver disease : role of oxidative metabolism", World J Gastroenterol, 20, pp.17756~17772 (2014)
- Ringseis R, "Dietary oxidized fat prevents ethanol-induced triacylglycerol accumulation and increases expression of PPARalpha target genes in rat liver", J Nutr, 137, pp.77~83 (2007)
- Masella R, "Novel mechanisms of natural antioxidant compounds in biological systems : involvement of glutathione and glutathione-related enzymes", J Nutr Biochem, 16, pp.577~586 (2005)
- Nanji AA, "Role of Kupffer cells in alcoholic hepatitis", Alcohol, 27, pp.13~15 (2002)
- Wheeler MD, "Endotoxin and Kupffer cell activation in alcoholic liver disease", Alcohol Res Health, 27, pp.300~306 (2003)
- Cesaro C, "Gut microbiota and probiotics in chronic liver diseases", Dig Liver Dis, 43, pp.431~438 (2011)
- Gao B, "Alcoholic liver disease : pathogenesis and new therapeutic targets", Gastroenterology, 141, pp.1572~1585 (2011)
- Nanji AA, "Lactobacillus feeding reduces endotoxemia and severity of experimental alcoholic liver(disease)", Proc Soc Exp Biol Med, 205, pp.243~247 (1994)
- Wang Y, "Lactobacillus rhamnosus GG treatment potentiates intestinal hypoxia-inducible factor, promotes intestinal integrity and ameliorates alcohol-induced liver injury", Am J Pathol, 179, pp.2866~2875 (2011)
- Kirpich IA, "Probiotics restore bowel flora and improve liver enzymes in human alcohol-induced liver injury : a pilot study", Alcohol, 42, pp.675~682 (2008)
- Gareau MG, "Probiotics and the gut microbiota in intestinal health and disease", Nat Rev Gastroenterol Hepatol, 7, pp.503~514 (2010)
- Hemarajata P, "Effects of probiotics on gut microbiota : mechanisms of intestinal immunomodulation and neuromodulation", Therap Adv Gastroenterol, 6, pp.39~51 (2013)
- Bull-Otterson L, "Metagenomic analyses of alcohol induced pathogenic alterations in the intestinal microbiome and the effect of Lactobacillus rhamnosus GG treatment", PLoS One, 8, pp.e53028~ (2013)
- Forsyth CB, "Lactobacillus GG treatment ameliorates alcohol-induced intestinal oxidative stress, gut leakiness, and liver injury in a rat model of alcoholic steatohepatitis", Alcohol, 43, pp.163~172 (2009)
- Wang Y, "Lactobacillus rhamnosus GG culture supernatant ameliorates acute alcohol-induced intestinal permeability and liver injury", Am J Physiol Gastrointest Liver Physiol, 303, pp.G32~G41 (2012)
- Folch J, "A simple method for the isolation and purification of total lipides from animal tissues", J Biol Chem, 226, pp.497~509 (1957)
- Ryu JE, "Evaluation of nonalcoholic fatty liver disease in C57BL", 6J mice by using MRI and histopathologic analyses, 2015, pp.65, 409~415
- Shackelford C, "Qualitative and quantitative analysis of nonneoplastic lesions in toxicology studies", Toxicol Pathol, 30, pp.93~96 (2002)
- Osna NA, "Alcoholic liver disease : pathogenesis and current management", Alcohol Res, 38, pp.147~161 (2017)
- Ahmed M, "Non-alcoholic fatty liver disease in 2015", World J Hepatol, 7, pp.1450~1459 (2015)
- Horton JD, "SREBPs : activators of the complete program of cholesterol and fatty acid synthesis in the liver", J Clin Invest, 109, pp.1125~1131 (2002)
- Clugston RD, "Altered hepatic lipid metabolism in C57BL", 6 mice fed alcohol : a targeted lipidomic and gene expression study, 2011, pp.52, 2021~2031
- Sun JR, "Antiobesity effects of lactic acid bacteria supplements on high-fat diet-induced obese rats and their molecular mechanisms", master, pp.2017
- Salvaggio A, "Body mass index and liver enzyme activity in serum", Clin Chem, 37, pp.720~723 (1991)
- Ewaschuk J, "Probiotic bacteria prevent hepatic damage and maintain colonic barrier function in a mouse model of sepsis", Hepatology, 46, pp.841~850 (2007)
- Gutiérrez-Ruiz MC, "Cytokines, growth factors, and oxidative stress in HepG2 cells treated with ethanol, acetaldehyde, and LPS", Toxicology, 134, pp.197~207 (1999)
- Clark VL, "Clinical methods : the history, physical, and laboratory examinations", JAMA, 264, pp.2808~2809 (1990)
- Giannini EG, "Liver enzyme alteration : a guide for clinicians", CMAJ, 172, pp.367~379 (2005)
- Segawa S, "Oral administration of heat-killed Lactobacillus brevis SBC8803 ameliorates alcoholic liver disease in ethanol-containing diet-fed C57BL", 6N mice, 2008, pp.128, 371~377
- Rao R, "Endotoxemia and gut barrier dysfunction in alcoholic liver disease", Hepatology, 50, pp.638~644 (2009)
- Patel D, "Potential molecular mechanism of probiotics in alcoholic liver disease", J Alcohol Drug Depend, 5, pp.278~ (2017)
- Ohland CL, "Probiotic bacteria and intestinal epithelial barrier function", Am J Physiol Gastrointest Liver Physiol, 298, pp.G807~19 (2010)
- Chen X, "Hepatoprotective effects of Lactobacillus on carbon tetrachloride-induced acute liver injury in mice", Int J Mol Sci, 19, pp.2212~ (2018)
- Leung TM, "CYP2E1 and oxidant stress in alcoholic and non-alcoholic fatty liver disease", J Hepatol, 58, pp.395~398 (2013)
- Salvi M, "Catalase takes part in rat liver mitochondria oxidative stress defense", J Biol Chem, 282, pp.24407~24415 (2007)