Renoprotective and antioxidant effects of Saururus chinensis Baill in rats fed a high-fructose diet
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This study investigated the preventive effect of Saururus chinensis Baill against renal damage induced by a high-fructose diet in rats. The rats (n = 30) were fed either a cornstarch-based (65%), high-fructose (65%), or high-fructose (64.5%) diet with 0.5% S. chinensis Baill extract for 10weeks. Twenty-four hour urine collections were obtained and the animals were sacrificed after an overnight fast. Serum urea and creatinine and urine albumin were measured using colorimetric methods, and creatinine clearance was determined. In addition, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and the activity of superoxide dismutase (SOD) in the kidney were determined. Kidney samples were also examined histologically. The fructose-fed rats showed renal dysfunction, indicated by decreased creatinine clearance, increased albumin in the urine,and increased urea and creatinine in the serum. These renal function parameters were comparable to control levels in rats that consumed S. chinensis Baill. Fructose consumption increased renal TBARS and reduced GSH and SOD activity, whereas these levels were near-normal in the rats consuming S. chinensis Baill. The kidneys of fructose-fed rats showed glomerular basement membrane thickening, mesangial matrix expansion, and tubule dilation. These pathological changes were not seen in the rats that consumed S. chinensis Baill. Therefore, S. chinensis Baill effectively alleviated fructose-induced renal damage in these rats, at least partially due to antioxidant activity.
Abstract
This study investigated the preventive effect of Saururus chinensis Baill against renal damage induced by a high-fructose diet in rats. The rats (n = 30) were fed either a cornstarch-based (65%), high-fructose (65%), or high-fructose (64.5%) diet with 0.5% S. chinensis Baill extract for 10weeks. Twenty-four hour urine collections were obtained and the animals were sacrificed after an overnight fast. Serum urea and creatinine and urine albumin were measured using colorimetric methods, and creatinine clearance was determined. In addition, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and the activity of superoxide dismutase (SOD) in the kidney were determined. Kidney samples were also examined histologically. The fructose-fed rats showed renal dysfunction, indicated by decreased creatinine clearance, increased albumin in the urine,and increased urea and creatinine in the serum. These renal function parameters were comparable to control levels in rats that consumed S. chinensis Baill. Fructose consumption increased renal TBARS and reduced GSH and SOD activity, whereas these levels were near-normal in the rats consuming S. chinensis Baill. The kidneys of fructose-fed rats showed glomerular basement membrane thickening, mesangial matrix expansion, and tubule dilation. These pathological changes were not seen in the rats that consumed S. chinensis Baill. Therefore, S. chinensis Baill effectively alleviated fructose-induced renal damage in these rats, at least partially due to antioxidant activity.
참고문헌 (34)
- Vuilleumier S, "Worldwide production of high-fructose syrup and crystalline fructose", Am J Clin Nutr, 58, pp.733~736S (1993)
- Gibney M, "Consumption of sugars", Am J Clin Nutr, 62, pp.178~193 (1995)
- Gersch MS, "Fructose, but not dextrose, accelerates the progression of chronic kidney disease", Am J Physiol Renal Physiol, 293, pp.1256~1261 (2007)
- Palanisamy N, "Effect of genistein, a soy isoflavone, on whole body insulin sensitivity and renal damage induced by a high-fructose diet", Ren Fail, 30, pp.645~654 (2008)
- Rajasekar P, "Renoprotective action of L-carnitine in fructose-induced metabolic syndrome", Diabetes Obes Metab, 10, pp.171~180 (2008)
- Nandhini AT, "Effect of taurine on biomarkers of oxidative stress in tissues of fructose-fed insulin-resistant rats", Singapore Med J, 46, pp.82~87 (2005)
- Chung BS, "Dictionary of Korean Folk Medicine", pp.813~814 (1990), Seoul: Young Lim Sa
- Lee WS, "Antioxidant activities of a new lignan and a neolignan from Saururus chinensis", Bioorg Med Chem Lett, 14, pp.5623~5628 (2004)
- Choi CW, "Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison", Plant Sci, 163, pp.1161~1168 (2002)
- Yu MH, "Effects of ethanol extract from Saururus chinensis (Bour.) Baill on lipid and antioxidant metabolisms in rats fed a high-fat diet", Nat Prod Res, 22, pp.275~283 (2008)
- Wang L, "The hepatoprotective and antifibrotic effects of Saururus chinensis against carbon tetrachloride induced hepatic fibrosis in rats", J Ethnopharmacol, 126, pp.487~491 (2009)
- Ohkawa H, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction", Anal Biochem, 95, pp.351~358 (1979)
- Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.248~254 (1976)
- Ellman GL, "Tissue sulfhydryl groups", Arch Biochem Biophys, 82, pp.70~77 (1959)
- Nagi MN, "Depletion of rat hepatic glutathione and inhibition of microsomal trans-2-enoyl-CoA reductase activity following administration of a dec-2-ynol and dec-2-ynoic acid", Arch Biochem Biophys, 293, pp.71~78 (1992)
- Marklund S, "Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase", Eur J Biochem, 47, pp.469~474 (1974)
- Kizhner T, "Long-term fructose intake: biochemical consequences and altered renal histology in the male rat", Metabolism, 51, pp.1538~1547 (2002)
- Yeong Ok Song, "Lipid-Lowering Effect of Hot Water-Soluble Extracts of Saururus chinensis Bail on Rats Fed High Fat Diets", Journal of Medicinal Food, 10(2), pp.316~322 (2007), 한국식품영양과학회
- Nakayama T, "Dietary fructose causes tubulointerstitial injury in the normal rat kidney", Am J Physiol Renal Physiol, 298, pp.712~720 (2010)
- Namnum P, "Evidence for bidirectional net movement of creatinine in the rat kidney", Am J Physiol, 244, pp.719~723 (1983)
- Aybar MJ, "Hypoglycemic effect of the water extract of Smallantus sonchifolius (yacon) leaves in normal and diabetic rats", J Ethnopharmacol, 74, pp.125~132 (2001)
- Schiffrin EL, "Endothelin: role in experimental hypertension", J Cardiovasc Pharmacol, 35, pp.33~35 (2000)
- 김정인, "Hypoglycemic and hypolipidemic effects of Saururus chinensis Baill in streptozotocin-induced diabetic rats", Nutrition Research and Practice, 1(2), pp.100~104 (2007), 한국영양학회
- Faure P, "improves the free radical defense system potential and insulin sensitivity of rats fed high fructose diets", J Nutr, 127, pp.103~107 (1997)
- Suzuki K, "The contribution of fructose and nitric oxide to oxidative stress in hamster islet tumor (HIT) cells through the inactivation of glutathione peroxidase", Electrophoresis, 21, pp.285~288 (2000)
- Fields M, "The influence of gender on developing copper deficiency and on free radical generation of rats fed a fructose diet", Metabolism, 41, pp.989~994 (1992)
- López-Novoa JM, "Role of reactive oxygen species in renal function and diseases", Antioxid Redox Signal, 4, pp.867~868 (2002)
- Basnakian AG, "Apoptotic pathways of oxidative damage to renal tubular epithelial cells", Antioxid Redox Signal, 4, pp.915~924 (2002)
- Pedraza-Chaverrí J, "Soy protein diet ameliorates renal nitrotyrosine formation and chronic nephropathy induced by puromycin aminonucleoside", Life Sci, 74, pp.987~999 (2004)
- Palanisamy N, "Renoprotective and blood pressure-lowering effect of dietary soy protein via protein kinase C βII inhibition in a rat model of metabolic syndrome", Can J Physiol Pharmacol, 88, pp.28~37 (2010)
- Pompella A, "The changing faces of glutathione, a cellular protagonist", Biochem Pharmacol, 66, pp.1499~1503 (2003)
- Harman D, "The aging process: major risk factor for disease and death", Proc Natl Acad Sci U S A, 88, pp.5360~5363 (1991)
- Kang TH, "Flavonol glycosides with free radical-scavenging activity of Saururus chinensis", Fitoterapia, 76, pp.115~117 (2005)
- Kim YW, "Efficacy of sauchinone as a novel AMPK-activating lignan for preventing iron-induced oxidative stress and liver injury", Free Radic Biol Med, 47, pp.1082~1092 (2009)
