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Study on the hypochlolesterolemic and antioxidative effects of tyramine derivatives from the root bark of Lycium chenese Miller

박은정|김은옥|최상원|조성희
한국영양학회 (The Korean Nutrition Society)|2011.10|Nutrition Research and Practice|제5권 제5호|pp. 412-420

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참고문헌

키워드

serum lipidHMG CoA reductaseLycii Cortex RadicistyramineACAT

초록

The aim of the present study was to investigate the hypocholesterolemic effect and potential of tyramine derivatives from Lycii Cortex Radicis (LCR), the root bark of lycium (Lycium chenese Miller) in reducing lipid peroxidation. The activities of enzymes, hepatic 3-hydroxy 3-methylglutaryl (HMG) CoA reductase and acyl-CoA:cholesterol acyltransferase (ACAT) and LDL oxidation were measured in vitro and animal experiments were also performed by feeding LCR extracts to rats. The test compounds employed for in vitro study were trans-N-p-coumaroyltyramine (CT) and trans-N-feruloyltyramine (FT), LCR components, N-(p-coumaroyl)serotonin (CS) and N-feruloylserotonin (FS) from safflower seeds, ferulic acid (FA)and 10-gingerol. It was observed that FT and FS at the concentration of 1.2 mg/mL inhibited liver microsomal HMG CoA reductase activity by ~ 40%, but no inhibition of activity was seen in the cases of CT, CS, FA and 10-gingerol. Whereas, ACAT activity was inhibited ~ 50% by FT and CT, 34-43% by FS and CS and ~80% by 10-gingerol at the concentration of 1 mg/mL. A significant delay in LDL oxidation was induced by CT, FT, and 10-gingerol. For the animal experiment, five groups of Sprague-Dawley male rats were fed high fat diets containing no test material (HF-control), 1 and 2% of LCR ethanol extract (LCR1 and LCR2), and 1% of extracts from safflower seed (Saf) and ginger (Gin). The results indicated that total cholesterol level was significantly lower in Saf, LCR2 and Gin groups, and HDL cholesterol level was lower only in Gin group when compared with HF-control group; while there was no difference in the serum triglyceride levels among the five experimental groups. The level of liver cholesterol was significantly lower in LCR1 and LCR2 groups than HF-control. Serum levels of TBARS were significantly lower only in LCR2 group when compared with HF-control group. From the observed results, we concluded that LCR can be utilized as a hypocholesterolemic ingredient in combination with ginger, especially for functional foods.

Abstract

The aim of the present study was to investigate the hypocholesterolemic effect and potential of tyramine derivatives from Lycii Cortex Radicis (LCR), the root bark of lycium (Lycium chenese Miller) in reducing lipid peroxidation. The activities of enzymes, hepatic 3-hydroxy 3-methylglutaryl (HMG) CoA reductase and acyl-CoA:cholesterol acyltransferase (ACAT) and LDL oxidation were measured in vitro and animal experiments were also performed by feeding LCR extracts to rats. The test compounds employed for in vitro study were trans-N-p-coumaroyltyramine (CT) and trans-N-feruloyltyramine (FT), LCR components, N-(p-coumaroyl)serotonin (CS) and N-feruloylserotonin (FS) from safflower seeds, ferulic acid (FA)and 10-gingerol. It was observed that FT and FS at the concentration of 1.2 mg/mL inhibited liver microsomal HMG CoA reductase activity by ~ 40%, but no inhibition of activity was seen in the cases of CT, CS, FA and 10-gingerol. Whereas, ACAT activity was inhibited ~ 50% by FT and CT, 34-43% by FS and CS and ~80% by 10-gingerol at the concentration of 1 mg/mL. A significant delay in LDL oxidation was induced by CT, FT, and 10-gingerol. For the animal experiment, five groups of Sprague-Dawley male rats were fed high fat diets containing no test material (HF-control), 1 and 2% of LCR ethanol extract (LCR1 and LCR2), and 1% of extracts from safflower seed (Saf) and ginger (Gin). The results indicated that total cholesterol level was significantly lower in Saf, LCR2 and Gin groups, and HDL cholesterol level was lower only in Gin group when compared with HF-control group; while there was no difference in the serum triglyceride levels among the five experimental groups. The level of liver cholesterol was significantly lower in LCR1 and LCR2 groups than HF-control. Serum levels of TBARS were significantly lower only in LCR2 group when compared with HF-control group. From the observed results, we concluded that LCR can be utilized as a hypocholesterolemic ingredient in combination with ginger, especially for functional foods.

참고문헌 (48)

  1. Endo A, "a new hypocholesterolemic agent that specifically inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase", J Antibiot (Tokyo), 33, pp.334~336 (1980)
  2. Endo A, "a new inhibitor of cholesterol biosynthesis", J Antibiot (Tokyo), 39, pp.1670~1673 (1986)
  3. Gunde-Cimerman N, "A hydroxymethylglutaryl- CoA reductase inhibitor synthesized by yeasts", FEMS Microbiol Lett, 132, pp.39~43 (1995)
  4. Grundy SM, "HMG-CoA reductase inhibitors for treatment of hypercholesterolemia", N Engl J Med, 319, pp.24~33 (1988)
  5. Qureshi AA, "The structure of an inhibitor of cholesterol biosynthesis isolated from barley", J Biol Chem, 261, pp.10544~10550 (1986)
  6. Gebhardt R, "Differential inhibitory effects of garlicderived organosulfur compounds on cholesterol biosynthesis in primary rat hepatocyte cultures", Lipids, 31, pp.1269~1276 (1996)
  7. Bok SH, "Plasma and hepatic cholesterol and hepatic activities of 3-hydroxy-3-methyl-glutaryl-CoA reductase and acyl CoA: cholesterol transferase are lower in rats fed citrus peel extract or a mixture of citrus bioflavonoids", J Nutr, 129, pp.1182~1185 (1999)
  8. 박용복, "Quercetin dihydrate and gallate supplements lower plasma and hepatic lipids and change activities of hepatic antioxidant enzymes in high cholesterol-fed rats", INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH, 72(3), pp.161~169, VERLAG HANS HUBER
  9. Ha TY, "Screening of HMG-CoA reductase inhibitory activity of ethanol and methanol extracts from cereals and regumes", Korean J Food Sci Technol, 30, pp.224~229 (1998)
  10. 조성희, "홍화씨 폴리페놀이 HMG-CoA reductase, LDL 산화 및Apo A1 분비에 미치는 영향", 한국식품과학회지, 38(2), pp.279~283 (2006), 한국식품과학회
  11. Valeriy V. Pak, "Hypocholesterolemic Soybean Peptide (IAVP) Inhibits HMG-CoAReductase in a Competitive Manner", Food Science and Biotechnology, 14(6), pp.727~731 (2005), 한국식품과학회
  12. Lee HJ, "Measurement of inhibitory activities on 3-hydroxy-3-methylglutaryl CoA reductase and acyl-CoA:cholesterol acyltransferase by various plant extracts in vitro", J Korean Soc Food Sci Nutr, 28, pp.958~962 (1999)
  13. Kim NJ, "Studies on the development of antihyperlipidemic drugs from oriental herbal medicines (I): antihyperlipidemic activities of oriental herbal medicines", Korean J Pharmacogn, 30, pp.368~376 (1999)
  14. Kim MK, "Screening of Acyl-CoA:cholesterol acyltransferase (ACAT) inhibitors from natural sources", Korean J Pharmacogn, 30, pp.384~396 (1999)
  15. Jin Hwan Lee, "Human Acyl-CoA: Cholesterol Acyltransferase Inhibitory Effect ofFlavonoids from Roots of Glycine max (L.) Merr", Journal of Applied Biological Chemistry, 49(2), pp.57~61 (2006), 한국응용생명화학회
  16. Geum-Soog Kim, "Human Acyl-CoA:Cholesterol Acyltransferase-inhibiting Dammarane Triterpenes from Rhus chinensis", Journal of the Korean Society for Applied Biological Chemistry, 53(4), pp.417~421 (2010), 한국응용생명화학회
  17. Kitayama K, "Importance of acyl-coenzyme A:cholesterol acyltransferase 1", 2 dual inhibition for anti-atherosclerotic potency of pactimibe, 2006, pp.540, 121~130
  18. Terasaka N, "ACAT inhibitor pactimibe sulfate (CS-505) reduces and stabilizes atherosclerotic lesions by cholesterol-lowering and direct effects in apolipoprotein E-deficient mice", Atherosclerosis, 190, pp.239~247 (2007)
  19. Ikenoya M, "A selective ACAT-1 inhibitor, K-604, suppresses fatty streak lesions in fat-fed hamsters without affecting plasma cholesterol levels", Atherosclerosis, 191, pp.290~297 (2007)
  20. Nissen SE, "ACAT Intravascular Atherosclerosis Treatment Evaluation (ACTIVATE) Investigators. Effect of ACAT inhibition on the progression of coronary atherosclerosis", N Engl J Med, 354, pp.1253~1263 (2006)
  21. Witzum JL, "The role of monocytes and oxidized LDL in atherosclerosis. In:Atherosclerosis Reviews, Vol. 21", pp.59~69 (1990), New York: Raven Press
  22. Dieber-Rotheneder M, "Effect of oral supplementation with D-α-tocopherol on the vitamin E content of human low density lipoproteins and resistance to oxidation", J Lipid Res, 32, pp.1325~1332 (1991)
  23. Meng QH, "Incorporation of esterified soybean isoflavones with antioxidant activity into low density lipoprotein", Biochim Biophys Acta, 1438, pp.369~376 (1999)
  24. Gao D, "Hypoglycemic effects and mechanisms of action of Cortex Lycii Radicis on alloxan-induced diabetic mice", Yakugaku Zasshi, 127, pp.1715~1721 (2007)
  25. Ahn BY, "Protective effect of water extract of Lycii Cordex Radicis on lipid peroxidation of rat skin exposed to ultraviolet B radiation", Agric Chem Biotechnol, 45, pp.218~222 (2002)
  26. Cho YJ, "Protective effect of EA fraction of Lycii Cortex Radix on the hepatic damage in mice induced by CCl4", Korean J Orient Med Pathol, 11, pp.63~71 (1997)
  27. 정현준, "Anti-fungal effects of phenolic amides isolated from the root bark of Lycium chinense", BIOTECHNOLOGY LETTERS, 26, pp.1125~1130, SPRINGER
  28. Kang GH, "Antioxidative activity of phenolic compounds in roasted safflower (Carthamus tinctorius L.) seeds", J Korean Soc Food Sci Nutr, 4, pp.221~225 (1999)
  29. Rajan P, "Synthesis and evaluation of caffeic acid amides as antioxidants", Bioorg Med Chem Lett, 11, pp.215~217 (2001)
  30. Kim EO, "Antilipidemic activity of phenolic compounds isolated from plant sources", pp.11~75 (2010), Abstracts from Annual Meeting of Korean Society of Food Sci & Technology
  31. Kleinsek DA, "3-Hydroxy-3- methylglutaryl coenzyme A reductase from rat liver. In:Methods in Enzymology, Vol. 71", pp.462~479 (1981), New York: Academic Press
  32. Hirano R, "Effects of antioxidants on the oxidative susceptibility of low-density lipoprotein", J Nutr Sci Vitaminol (Tokyo), 43, pp.435~444 (1997)
  33. Woo MN, "Hypolipidemic and body fat-lowering effects of Fatclean in rats fed a high-fat diet", Food Chem Toxicol, 47, pp.2076~2082 (2009)
  34. 양수영, "레시틴섭취가 고지방 식이를 섭취한 흰쥐의 지방대사와항산화능에 미치는 영향", 한국영양학회지, 40(4), pp.312~319 (2007), 한국영양학회
  35. Reeves PG, "AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet", J Nutr, 123, pp.1939~1951 (1993)
  36. Cho SH, "Effects of defatted safflower seed extract and phenolic compounds in diet on plasma and liver lipid in ovariectomized rats fed highcholesterol diets", J Nutr Sci Vitaminol (Tokyo), 50, pp.32~37 (2004)
  37. Yagi K, "A simple fluorometric assay for lipoperoxide in blood plasma", Biochem Med, 15, pp.212~216 (1976)
  38. Chow LW, "Antibacterial activity of burdock", Food Sci, 24, pp.195~202 (1997)
  39. Roberfroid MB, "Dietary fructans", Annu Rev Nutr, 18, pp.117~143 (1998)
  40. 성혜영, "고콜레스테롤 식이 섭취 흰쥐에서 치커리 이눌린과 올리고당이 지질대사에 미치는 영향", 한국식품영양과학회지, 33(2), pp.305~310 (2004), 한국식품영양과학회
  41. 허보영, "당뇨환자를 위한 올리고당 첨가 콩아이스크림：품질특성과 당뇨 흰쥐에서의혈당 및 지질 개선에 미치는 효과", 한국영양학회지, 38(8), pp.663~671 (2005), 한국영양학회
  42. 이연숙, "Effects of sn-2 palmitic acid-fortified vegetable oil and fructooligosaccharide on calcium metabolism in growing rats fed casein based diet", Nutrition Research and Practice, 2(1), pp.3~7 (2008), 한국영양학회
  43. Un Ju Jung, "Antilipogenic and Hypolipidemic Effects of Ethanol Extracts from Two Variants of Artemisia princeps Pampanini in Obese Diabetic Mice", Journal of Medicinal Food, 12(6), pp.1238~1244 (2009), 한국식품영양과학회
  44. Kwon EY, "Antiatherogenic property of ferulic acid in apolipoprotein E-deficient mice fed Western diet: comparison with clofibrate", Food Chem Toxicol, 48, pp.2298~2303 (2010)
  45. Sri Balasubashini M, "Protective effects of ferulic acid on hyperlipidemic diabetic rats", Acta Diabetol, 40, pp.118~122 (2003)
  46. - gingerol: Its potential roles in cancer chemoprevention, "Biofactors", 2010, 169~178
  47. P-induced mouse skin tumorigenesis, "Cancer Chemother Pharmacol", 2010, 687~696
  48. -shogaol, "J Ethnopharmacol", 2010, 515~520

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