Sasa borealis extract exerts an antidiabetic effect via activation of the AMP-activated protein kinase
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Leaf of Sasa borealis, a species of bamboo, has been reported to exhibit anti-hyperglycemic effect. However, its antidiabetic mechanism is not fully understood. In this study, we examined whether an extract of S. borealis activates AMP-activated protein kinase (AMPK) and exerts anti-hyperglycemic effects. Treatment with the S. borealis extract increased insulin signaling and phosphorylation of AMPK and stimulated the expression of its downstream targets, including PPARα, ACO, and CPT-1 in C2C12 cells and PPARα in HepG2 cells. However, inhibition of AMPK activation attenuated insulin signaling and prevented the stimulation of AMPK target genes. The S. borealis extract increased glucose uptake in C2C12 cells and suppressed expression of the gluconeogenic gene, PEPCK in HepG2 cells. The extract significantly reduced blood glucose and triglyceride levels in STZ-induced diabetic mice. The extract enhanced AMPK phosphorylation and increased Glut-4 expression in the skeletal muscle of the mice. These findings demonstrated that the S. borealis extract exerts its anti-hyperglycemic effect through activation of AMPK and enhancement of insulin signaling.
Abstract
Leaf of Sasa borealis, a species of bamboo, has been reported to exhibit anti-hyperglycemic effect. However, its antidiabetic mechanism is not fully understood. In this study, we examined whether an extract of S. borealis activates AMP-activated protein kinase (AMPK) and exerts anti-hyperglycemic effects. Treatment with the S. borealis extract increased insulin signaling and phosphorylation of AMPK and stimulated the expression of its downstream targets, including PPARα, ACO, and CPT-1 in C2C12 cells and PPARα in HepG2 cells. However, inhibition of AMPK activation attenuated insulin signaling and prevented the stimulation of AMPK target genes. The S. borealis extract increased glucose uptake in C2C12 cells and suppressed expression of the gluconeogenic gene, PEPCK in HepG2 cells. The extract significantly reduced blood glucose and triglyceride levels in STZ-induced diabetic mice. The extract enhanced AMPK phosphorylation and increased Glut-4 expression in the skeletal muscle of the mice. These findings demonstrated that the S. borealis extract exerts its anti-hyperglycemic effect through activation of AMPK and enhancement of insulin signaling.
참고문헌 (23)
- Cheng AY, "Oral antihyperglycemic therapy for type 2 diabetes mellitus", CMAJ, 172, pp.213~226 (2005)
- Colberg SR, "Postprandial walking is better for lowering the glycemic effect of dinner than pre-dinner exercise in type 2 diabetic individuals", J Am Med Dir Assoc, 10, pp.394~397 (2009)
- Yin J, "Traditional Chinese medicine in treatment of metabolic syndrome", Endocr Metab Immune Disord Drug Targets, 8, pp.99~111 (2008)
- Bailey CJ, "Traditional plant medicines as treatments for diabetes", Diabetes Care, 12, pp.553~564 (1989)
- Lu B, "Toxicology and safety of antioxidant of bamboo leaves. Part 2: developmental toxicity test in rats with antioxidant of bamboo leaves", Food Chem Toxicol, 44, pp.1739~1743 (2006)
- 김은영, "조릿대 잎 추출물이 고지방식이 유도 비만 마우스 (C57", BL6J)의혈장 Adiponectin, Resistin, C-reactive Protein 및Homocysteine 농도에 미치는 영향, 2007, pp.40(4), 303~311[DOI]
- 고병섭, "In vitro에서 조릿대, 연근과 연잎이 인슐린 작용 및 분비에 미치는 영향", 한국식품과학회지, 38(1), pp.114~120 (2006), 한국식품과학회
- Choi YJ, "Blockade of chronic high glucose-induced endothelial apoptosis by Sasa borealis bamboo extract", EXPERIMENTAL BIOLOGY AND MEDICINE, 233(5), pp.580~591 (2008), SOC EXPERIMENTAL BIOLOGY MEDICINE
- 한지숙, "조릿대잎 추출물의 탄수화물 소화효소활성 저해 및 식후혈당강하효과", 한국식품영양과학회지, 36(8), pp.989~994 (2007), 한국식품영양과학회
- Park EJ, "The antioxidant, angiotensin converting enzyme inhibition activity, and phenolic compounds of bamboo shoot extracts", Lebenson Wiss Technol, 43, pp.655~659 (2010)
- Zhang Y, "Anti-fatigue activity of a triterpenoid-rich extract from Chinese bamboo shavings (Caulis bamfusae in taeniam)", Phytother Res, 20, pp.872~876 (2006)
- DuBok Choi, "Effect of bamboo oil on antioxidative activity and nitrite scavenging activity", Journal of Industrial and Engineering Chemistry, 14(6), pp.765~770 (2008), 한국공업화학회
- 양정화, "Sasa borealis leaves extract improves insulin resistance by modulating inflammatory cytokine secretion in high fat diet-induced obese C57", BL6J mice, 2010, pp.4(2), 99~105[DOI]
- Carling D, "The AMP-activated protein kinase cascade--a unifying system for energy control", Trends Biochem Sci, 29, pp.18~24 (2004)
- Hardie DG, "Role of AMP-activated protein kinase in the metabolic syndrome and in heart disease", FEBS Lett, 582, pp.81~89 (2008)
- Lee MJ, "Effect of bamboo culm extract on oxidative stress and genetic expression: bamboo culm extract ameliorates cell adhesion molecule expression and NFκB activity through the suppression of the oxidative stress", Clin Nutr, 27, pp.755~763 (2008)
- Shaw RJ, "The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress", Proc Natl Acad Sci U S A, 101, pp.3329~3335 (2004)
- Hurley RL, "The Ca2+", calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases, 2005, pp.280, 29060~29066
- Cartee GD, "Role of Akt substrate of 160 kDa in insulin-stimulated and contraction-stimulated glucose transport", Appl Physiol Nutr Metab, 32, pp.557~566 (2007)
- Watson RR, "Bamboo extract in the prevention of diabetes and breast cancer, In Complementary and Alternative Therapies and the Aging Population: An Evidence-Based Approach", pp.159~177 (2009), Elsevier
- Jung HJ, "19α-hydroxyursane- type triterpenoids: antinociceptive anti-inflammatory principles of the roots of Rosa rugosa", Biol Pharm Bull, 28, pp.101~104 (2005)
- Hae-Suk Park, "Antioxidant Flavone Glycosides from the Leaves of Sasa borealis", Archives of Pharmacal Research, 30(2), pp.161~166 (2007), 대한약학회
- Mahato SB, "Triterpenoids", Phytochemistry, 31, pp.2199~2249 (1992)