생장점 배양에 의한 카네이션 무병주 생산
Virus free Healthy plant production through Meristem culture in carnation (Dianthus caryophillus)
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Abstract
This study was conducted to obtain the virus free plants through meristem culture ofcarnation (Dianthus caryophillus). Four cultivars (Roland, Desio, Casha, Giant Gipsy) werecollected for materials. The apical meristem 0.3-0.5 mm in size was cultured on MS mediumcontaining 3% sucrose, 0.9% agar at pH 5.8 with various plant growth regulators for 7weeks. Among the cultivars, Giant Gipsy had a better response than other cultivars in shootformation and reduced vitrification. Callus induction and shoot formation from themeristem culture were influenced by the various kinds of cytokine. Kinetin supplement wasthe most effective for shoot formation and NAA addition was good for callus inductionamong the treatments. Total 115 plantlets derived from apical meristem culture werechecked for CarMV and CarRSV infection by ELISA test. Among them, 40 plantlets(34.8%) were infected with CarMV but not detected for CarRSV.
참고문헌 (9)
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