Enhanced Seed Development in the Progeny from the Interspecific Backcross (Fagopyrum esculentum X F. homotropicum) X F. esculentum
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
To facilitate the introgression of F. esculentum into the traits of F. homotropicum, several accessions of the hybrids between these two species were pollinated with F. esculentum as the recurrent parent. The embryo in vitro rescue was performed to increase the recovery of backcross progenies. The F2 generation was more amenable than F1 hybrids to produce backcross progenies. The F1 hybrids were backcrossed twice with common buckwheat (pin-type F. esculentum) (recurrent backcrossing). Also, alternate backcrosses with common buckwheat and F. homotropicum (congruity backcrossing) were carried out. Pollen tube growth of BCF1 x F. esculentum (thrum) and F. homotropicum x BCF1 was the disturbed penetration exceeded for all initial interspecific hybrids, and its requirement was proportionally lower when the common buckwheat was used as the recurrent parent and as the last parent of congruity hybrids. Effects of both common buckwheat and F. homotropicum on seed success rate for hybridization were observed. Growth of hybrid embryos before rescue, regeneration of mature hybrids all increased recurrent and congruity backcrosses and inter-crosses between F1 plants and selected fertile plants of the second congruity backcrosses.
Abstract
To facilitate the introgression of F. esculentum into the traits of F. homotropicum, several accessions of the hybrids between these two species were pollinated with F. esculentum as the recurrent parent. The embryo in vitro rescue was performed to increase the recovery of backcross progenies. The F2 generation was more amenable than F1 hybrids to produce backcross progenies. The F1 hybrids were backcrossed twice with common buckwheat (pin-type F. esculentum) (recurrent backcrossing). Also, alternate backcrosses with common buckwheat and F. homotropicum (congruity backcrossing) were carried out. Pollen tube growth of BCF1 x F. esculentum (thrum) and F. homotropicum x BCF1 was the disturbed penetration exceeded for all initial interspecific hybrids, and its requirement was proportionally lower when the common buckwheat was used as the recurrent parent and as the last parent of congruity hybrids. Effects of both common buckwheat and F. homotropicum on seed success rate for hybridization were observed. Growth of hybrid embryos before rescue, regeneration of mature hybrids all increased recurrent and congruity backcrosses and inter-crosses between F1 plants and selected fertile plants of the second congruity backcrosses.
참고문헌 (22)
- T. Adachi, "How to combine the reproductive system with biotechnology in order to overcome the breeding barriers in buckwheat", Fagopyrum, 10, pp.7~11 (1990)
- T. Adachi, "Observation of pollen tube elongation, fertilization and ovule development in autogamous autotetraploid buck-wheat", Proc. 2nd Intl. Symp. on Buckwheat, pp.103~113 (1983)
- C. Campbell, "Inter-specific hybridization in the genus Fagopyrum", Curr. Adv. Buckwheat Res., 6, pp.255~263 (1995)
- O. L. Gamborg, "Nutrient requirements of suspension cultures of soybean root cells", Exp. Cell Res., 50, pp.151~158 (1968)
- L. M. Guan, "Reproductive deterioration in buckwheat (Fagopyrum esculentum) under summer conditions", Plant Breed, 109, pp.304~312 (1992)
- J. M. Herr, "An analysis of methods for permently mounting ovules cleared in four-and-harf type clearing fluids", Stain Technology, 57(3), pp.161~169 (1982)
- T. Hirose, "Pollen tube behavior related to self-incompatibility in interspecific crosses of Fagopyrum. Breed", Sci., 45, pp.65~70 (1995)
- I. Kreft, "Buckwheat breeding perspectives In: Buckwheat Research", Proc. 2nd Intl. Symp. Buckwheat Res., pp.113~139 (1983)
- A. S. Krotov, "An amphidiploid buckwheat, Fagopyrum giganteum, Krot sp. Nova. Vavilva", From Plant Breed. Abst., 30, pp.41~45 (1975)
- H. G. Marshall, "Buckwheat: description, breeding, production, and utilization", Adv. Cereal Sci. Tech., 5, pp.157~210 (1982)
- M. R. Morris, "Cytogenetic studies on buckwheat", J. Heredity, 42, pp.85~89 (1951)
- T. Murashige, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol. Plant, 15, pp.473~497 (1962)
- T. Nagatomo, "Studies on physiology of reproduction and some case of inheritance in buckwheat", Miyazaki University, ~ (1961)
- C. Samimy, "Barrier to interspecific crossing of Fagopyrum esculentum with Fagopyrum tataricum. I. Site of pollen-tube arrest. II. Organogenesis from immature embryos of F. tataricum", Euphytica, 54, pp.215~219 (1991)
- C. Samimy, "Overcoming the barrier to interspecific hybridization of Fagopyrum esculentum with Fagopyrum tataricum", Euphytica, 91, pp.323~330 (1996)
- A. Ujihara, "Interspecific hybridization in genus Fagopyrum-properties of hybrids (F.esculentum Moench. × F. cymosum Meissner) through ovule culture", Gamma Field Symp, pp.45~51 (1990), Inst. Radiation Breeding
- T. Wagatsuma, "In vitro culture of interspecific ovule between buckwheat (F. esculentum) and tartary (F. tataricum)", Breed. Sci., 45(2), pp.312~ (1995)
- Y. J. Wang, "Interspecific hybridization in buckwheat among Fagopyrum esculentum, F. homotropicum and F. tataricum", Adv. Buckwheat Res., 7, pp.11~12 (1998)
- S. H. Woo, "Possibility of interspecific hybridization by embryo rescue in the genus Fagopyrum", Curr. Adv. Buckwheat Res., 6, pp.225~237 (1995)
- S. H. Woo, "Production of interspecific hybrids between Fagopyrum esculentum and F. homotropicum through embryo rescue", SABRAO J., pp.89~95 (1997)
- S. H. Woo, "Inheritance of self-compatibility and flower morphology in interspecific buckwheat hybrids", Can. J. Plant Sci., 79, pp.483~490 (1999)
- S. H. Woo, "Pollen-tube behavior and embryo development in interspecific crosses among the genus Fagopyrum", J. of Plant Biology, 52, pp.302~310 (2008)