Assessment of Genetic Diversity and Fatty acid Composition of Perilla (Perilla frutescens var. frutescens) Germplasm
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초록
The objective of this study was to analyze the genetic diversity using SSR marker and investigate the fatty acid composition of perilla (P. frutescens var. frutescens) germplasm. Genetic diversity among 95 accessions, which consisted of 29 weedy types and 66 landrace accessions, was evaluated based on 12 SSR markers carrying 91 alleles. The mean values of observed (HO) and expected heterozygosities (HE) were 0.574 and 0.640, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into two distinct groups, which were the landrace, moderate and weedy type, genetic distance (GD) value was 0.609. The physicochemical traits about crude oil contents and fatty acid compositions were analyzed using GC. Among tested germplasm, the total average oil contents (%)showed a range from 28.57 to 49.67 %. Five fatty acids and their contents in the crude oils are as follows: α-linolenic acid (41.12%-51.81%), linoleic acid (15.38%-16.43%), oleic acid (18.93%-27.28%), stearic acid (2.56%-4.01%), and palmitic acid (7.38%-10.77%). The average oil content of wild types was lower than landrace, and the oil content of middle genotype accessions was higher than other germplasm, but no significant variation between landrace and wild types was shown. Nevertheless, IT117174, landrace of Korea, was highest in crude oil content (47.11%) and linolenic acid composition (64.58%) among the used germplasm. These traits of the selected accessions will be helped for new functional plant breeding in perilla crop.
Abstract
The objective of this study was to analyze the genetic diversity using SSR marker and investigate the fatty acid composition of perilla (P. frutescens var. frutescens) germplasm. Genetic diversity among 95 accessions, which consisted of 29 weedy types and 66 landrace accessions, was evaluated based on 12 SSR markers carrying 91 alleles. The mean values of observed (HO) and expected heterozygosities (HE) were 0.574 and 0.640, respectively, indicating a considerable amount of polymorphism within this collection. A genetic distance-based phylogeny grouped into two distinct groups, which were the landrace, moderate and weedy type, genetic distance (GD) value was 0.609. The physicochemical traits about crude oil contents and fatty acid compositions were analyzed using GC. Among tested germplasm, the total average oil contents (%)showed a range from 28.57 to 49.67 %. Five fatty acids and their contents in the crude oils are as follows: α-linolenic acid (41.12%-51.81%), linoleic acid (15.38%-16.43%), oleic acid (18.93%-27.28%), stearic acid (2.56%-4.01%), and palmitic acid (7.38%-10.77%). The average oil content of wild types was lower than landrace, and the oil content of middle genotype accessions was higher than other germplasm, but no significant variation between landrace and wild types was shown. Nevertheless, IT117174, landrace of Korea, was highest in crude oil content (47.11%) and linolenic acid composition (64.58%) among the used germplasm. These traits of the selected accessions will be helped for new functional plant breeding in perilla crop.
참고문헌 (31)
- Mohammad Asif, "Health effects of omega-3,6,9 fatty acids: Perilla frutescens is a good example of plant oils", Oriental Pharmacy and Experimental Medicine, 11(1), pp.51~59 (2011), 한의학연구소
- Christensen, S., "AFLP analysis of genetic diversity in leafy kale (Brassica oleracea L. convar. acephala ( DC.) Alef) landraces, cultivars and wild populations in Europe", Genet. Resour. Crop Evol, 58, pp.657~666 (2011)
- DeWoody, J.A, "Microsatellite markers in white-tailed deer", J. Hered, 86, pp.317~319 (1995)
- Ding, Y., "Characterization of fatty acid composition from five perilla seed oils in China and its relationship to annual growth temperature", J. Medicinal Plants Res, 6(9), pp.1645~1651 (2012)
- Dixit, A., "velopment of polymorphic microsatellite markers in sesame (Sesamum indicum L.)", Mol. Ecol. Notes, 5, pp.736~738 (2005)
- Ganapathy, K. N., "AFLP analysis in pigeonpea (Cajamus cajan (L.) Millsp.) revealed close relationship of cultivated genotypes with some of its wild relatives", Genet. Resour. Crop Evol, 58, pp.837~847 (2011)
- Gunstone, F. D., "The Lipid Handbook (2nd ed.)" (1994), Chapman & Hall
- Ito, M., "Phylogenetic analysis of Japanese perilla species by using DNA Polymorphims", Nat. Med, 52(3), pp.248~252 (1998)
- Ito, M., "Chemical composition of essential oil of Perilla frutescens", Nat. Med, 53, pp.32~36 (1999)
- Ito, M., "A new type of essential oil from Perilla frutescens from Thailand", J. Essent. Oil Res, 14, pp.416~419 (2002)
- Kim, H. K., "Suppression of hepatic fatty acid synthase by feeding a-linolenic acid rich Perilla oil lowers plasma triacyglycerol level in rats", J. Nutr. Biochem, 15, pp.485~492 (2004)
- Kopecky, J., "n-3 PUFA: Bioavailability and modulation of adipose tissue function", Proc. Nutr. Soc, 68(4), pp.361~369 (2009)
- Lee, J.K., "Geographic differentiation of morphological characters among perilla crops and their weedy types in East Asia", Breed Sci, 51, pp.247~255 (2001)
- Lee, J.K., "Genetic relationships among cultivated types of Perilla frutescens and their weedy types in East Asia revealed by AFLP markers", Genet. Resour. Crop Evol, 50, pp.65~74 (2003)
- Lee, J. K., "Genetic diversity of Perilla and related weedy types in Korea determined by AFLP analyses", Crop Sci, 42, pp.2161~2166 (2002)
- 이주경, "Analysis of Genetic Diversity and Relationships of Cultivated and Weedy Types of Perilla frutescens Collected from Korea by Using Microsatellite Markers", Genes & Genomics, 29(1), pp.81~89 (2007), 한국유전학회
- Li, X. W., "Flora Reipublicae Popularis Sinicae", Sci. Press, 65, pp.282~287 (1974)
- Liu, K., "PowerMarker: an integrated analysis environment for genetic marker analysis", Bioinformatics, 21, pp.2128~2129 (2005)
- Nitta M., "Genetic relationships among two perilla crops shiso and egoma and the weedy type revealed by RAPD markers", Genes Genet. Syst, 74, pp.43~48 (1999)
- Nitta, M., "Asian Perilla crops and theie weedy from : their cultivation utilization and genetic relationships", Econ. Bot, 57, pp.245~253 (2003)
- Natta, M., "The distribution of perilla species", Genet. Resour. Crop Evol, 52, pp.797~840 (2005)
- Okamoto, M., "Effects of perilla seed oil supplementation on leukotriene generation by leucocytes in patients with asthma associated with lipometabolism", Int. Arch Allergy Immunol, 122(2), pp.137~142 (2000)
- Park, Y. J., "Evaluation of genetic diversity and relationships wothin an on-farm collection of Perilla frutescens (L.) Britt. using microsatellite markers", Genet. Resour. Crop Evol, 55, pp.523~535 (2008)
- Peakall, R., "GENEALEX 6; Genetic analysis in Excel. Population genetic software for teaching and research", Mol. Ecol. Notes, 6, pp.288~295 (2006)
- Pritchard, J., "Inference of population structure using multilocus genotype data", Genetics, 155, pp.945~959 (2000)
- Pritchard, J.K., "Documentation for STRUCTURE software" (2004), The University of Chicago Press
- Rao, N. K., "Plant genetic resources: Advancing conservation and use through biotechnology", African J. Biotechnology, 3(2), pp.136~145 (2004)
- Russo, G.L., "Dietary n-6 and n-3 polyunsaturated fatty acids: From biochemistry to clinical implications in cardiovascular prevention. Biochem", Pharmacol, 77(6), pp.937~946 (2009)
- Siriamornpun, S., "Variation of lipid and fatty acid compositions in Thaiperilla seeds grown at different locations", J. Song Sci. Tech, 28, pp.17~21 (2006)
- landraces using STMS markers, "Indian J. Biotech", 2010, 43~49
- Villa, T. C. C., "Defining and identifying crop landraces", Plant Genet.Resour, 3, pp.373~384 (2006)