가지과식물에서 Capsidiol 생합성에 관여하는Cytochrome P450 유전자의 발현과 효소활성
Enzyme Activity and Gene Expression of Cytochrome P450 Involved inCapsidiol Biosynthesis in Solanaceae Plants
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Enzyme activity and expression of cytochrome P450 gene involved in the pathway of capsidiol biosynthesis were compared in five different solanaceae plants such as red pepper, green pepper, tobacco, potato and egg plant. Base on genomic DNA and/or RT-PCR results, four solanaceae plants such as red pepper, green pepper, tobacco and egg plant possess P450 gene in the genome and specifically expressed by elicitor treatment. However, potato was appeared to have neither P450 nor cyclase gene in the genome. P450 genes did not show any expression in the plants under normal condition, but showed highly specific expression under elicitation condition in various organs and tissue such as leaf, root, stem and culture cells.
Abstract
Enzyme activity and expression of cytochrome P450 gene involved in the pathway of capsidiol biosynthesis were compared in five different solanaceae plants such as red pepper, green pepper, tobacco, potato and egg plant. Base on genomic DNA and/or RT-PCR results, four solanaceae plants such as red pepper, green pepper, tobacco and egg plant possess P450 gene in the genome and specifically expressed by elicitor treatment. However, potato was appeared to have neither P450 nor cyclase gene in the genome. P450 genes did not show any expression in the plants under normal condition, but showed highly specific expression under elicitation condition in various organs and tissue such as leaf, root, stem and culture cells.
참고문헌 (11)
- Chappell, J., "Biochemistry and molecular biology of the isoprenoid biosynthetic pathway in plants", Annu. Rev. Plant Physiol. Plant Mol. Biol, 46, pp.521~547 (1995)
- Chappell, J., "Induction of sesquiterpenoid biosynthesis in tobacco cell suspension cultures by fungal elicitor", Plant Physiol, 85, pp.469~473 (1987)
- Donaldson, R.P., "Multiple forms of plant cytochrome P-450", Plant Physiol, 96, pp.669~674 (1991)
- Doo, H.S., "Trans-resveratrol content of peanut seeds depends on varieties and processing method", Kor. J. Plant Res, 20, pp.553~557 (2007)
- Lee, E., "Anti-flammatory effects of Scutellariae radix", Kor. J. Plant Res, 20, pp.548~552 (2007)
- Facchini, P.J., "Gene family for an elicitor-induced sesquiterpene cyclase in tobacco", Pro. Natl. Acad. Sci. USA, 89, pp.11088~11092 (1992)
- Hoshino, T., "5-epi-Aristolochene 3- hydroxylase from green pepper", Phytochemistry, 38, pp.609~613 (1995)
- Kato, H., "Characterization of an inducible P450 hydroxylase involved in the rice diterpene phytoalexin biothynthetic pathway", Arch. Biochem. Biophys, 316, pp.702~712 (1995)
- Kwon, S., "Elicitor-inducible 5-epi-aristolochene hydroxylase in suspension cultures of tobacco(Nicotiana tabacum L.)", Kor. J. Plant Tis. Cult, 25, pp.141~146 (1998)
- Kwon, S., "Cloning of cytochrome P450 gene involved in the pathway of capsidiol biosynthesis in red pepper cells", Kor. J. of Life Sci, 23, pp.879~888 (2003)
- Schuler, M.A., "Plant cytochrome P450 monooxygenases", Critical Rev. Plant Sci, 15, pp.235~284 (1996)