Inhibitory Effects of the Extracts from Eleutherococcus senticosus Maxim. on Histamine-release from Rat’s Mast Cell
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Eleutherococcus senticosus Maxim. has been successfully used as an oriental medicine for various diseases including allergic disorders. Histamine is a major factor on various allergic responses and it is reported that histamine was released from mast cells by sensitization of allergens. In this study, ethanol extracts from E. senticosus Maxim. were prepared and the composition was analyzed by high performance liquid chromatography. The eleutheroside B as a primary effective component of E. senticosus was contained approximately 225 mg/kg in root bark extracts. The extracts were found to significantly inhibit compound 48/80-induced histamine release form mast cells in dose dependent manner. However the extracts had low cytotoxicity on the mast cells with MTT assay. These results showed that E. senticosus Maxim. extracts may be the effective materials on inflammatory disorders.
Abstract
Eleutherococcus senticosus Maxim. has been successfully used as an oriental medicine for various diseases including allergic disorders. Histamine is a major factor on various allergic responses and it is reported that histamine was released from mast cells by sensitization of allergens. In this study, ethanol extracts from E. senticosus Maxim. were prepared and the composition was analyzed by high performance liquid chromatography. The eleutheroside B as a primary effective component of E. senticosus was contained approximately 225 mg/kg in root bark extracts. The extracts were found to significantly inhibit compound 48/80-induced histamine release form mast cells in dose dependent manner. However the extracts had low cytotoxicity on the mast cells with MTT assay. These results showed that E. senticosus Maxim. extracts may be the effective materials on inflammatory disorders.
참고문헌 (26)
- Davydov, M., "Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (Araliaceae) as an adaptogen: a closer look", J. Ethnopharmacology, 72, pp.345~393 (2000)
- Dubrac, S., "Atopic dermatitis: the role of Langerhans cells in disease pathogenesis", Immunol Cell. Biol, 88, pp.400~409 (2010)
- Fujikawa, T., "Acanthopanax senticosus Harms as a prophylactic for MPTP-induced Parkinson’'s disease in rats", J. Ethnopharmacology, 97, pp.375~381 (2005)
- Hachisuka, H., "Purification of rat cutaneous mast cells with Percoll density centrifugation", Arch. Dermatol. Res, 280, pp.358~362 (1988)
- Hikino, H., "Isolation and hypoglycemic activity of eleutherans A, B, C, D, E, F, and G: glycans of Eleutherococcus senticosus roots", J. Nat. Prod, 49, pp.293~297 (1986)
- Jang, M.H., "Protective effect of Acanthopanax senticosus against ethanol-induced apoptosis of human neuroblastoma cell line SK-N-MC", American J. Chin. Med, 31(3), pp.379~388 (2003)
- Jeong, H.J., "Inhibitory effects of mast cell-mediated allergic reactions by cell cultured Siberian Ginseng", Immunopharmacol Immunotoxicol, 23(1), pp.107~117 (2001)
- Jung, H.J., "In vivo anti-inflammatory and antinociceptive effects of liriodendrin isolated from the stem bark of Acanthopanax senticosus", Planta Medica, 69, pp.610~616 (2003)
- Kim, H.M., "Inhibitory effect of mast cell-mediated immediate-type allergic reactions in rats by spirulina", Biochemical Pharmacology, 55, pp.1071~1076 (1998)
- Kim, H.M., "Role of TGF-1 on the IgEdependent anaphylaxis reaction", J. Immunology, 162, pp.4960~4965 (1999)
- Kim, J.S., "Cytotoxicity of Vibrio vulnificus cytolysin on rat peritoneal mast cells", Microbiol Immunol, 42(12), pp.837~843 (1998)
- Kim, S.K., "Comparison of biological activity according to extracting solvents of four Acanthopanax root bark", Korean J. Medicinal Crop Sci, 8(1), pp.21~28 (2000)
- Kim, Y.J., "Eleutheroside E Content in Eleutherococcus spp", Korean J. Medicinal Crop Sci, 4(4), pp.333~339 (1996)
- 이상현, "오갈피나무의 부위별 Eleutheroside B와 Eleutheroside E의 정량", 생약학회지, 36(2), pp.70~74 (2005), 한국생약학회
- Lin, Q.Y., "Acanthopanax senticosus inhibits nitric oxide production in murine macrophages in vitro and in vivo", Phytother Res, 21(9), pp.879~883 (2007)
- Lin, Q.Y., "Acanthopanax senticosus suppresses reactive oxygen species production by mouse peritoneal macrophages in vitro and in vivo", Phytother Res, 22(6), pp.740~745 (2008)
- Metcalfe, D.D., "The mast cell", Critical Reviews in Immunology, 3, pp.23~74 (1981)
- Mosmann, T., "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", J. Immunol. Methods, 65(1), pp.55~63 (1983)
- Nakazato, J., "Serum levels of Th2 chemokines, CCL17, CCL22, and CCL27, were the important markers of severity in infantile atopic dermatitis", Pediatr. Allergy. Immunol, 19, pp.605~613 (2008)
- Nishibe, S., "Phenolic compounds from stem bark of Acanthopanax senticosus and their pharmacological effect in chronic swimming stressed rats", Chemical and Pharmaceutical Bulletin, 38, pp.1763~1765 (1990)
- Niu H.S., "Hypoglycemic Effect of Syringin from Eleutherococcus senticosus in Streptozotocin-Induced Diabetic Rats", Planta Medica, 74, pp.109~113 (2008)
- Petersen, L.J., "Allergen-induced histamine release in intact human skin in vivo assessed by skin microdialysis technique: characterization of factors influencing histamine releasability", J. Allergy and Clinical Immunology, 97, pp.672~679 (1996)
- Simpson, E.L., "Atopic dermatitis: a review of topical treatment options", Curr. Med. Res. Opin, 26, pp.633~640 (2010)
- Tokiwa, T., "Anti-inflammatory effect of eleuthreoside E from Acanthopanax senticosus", Foods and Food Ingredients J. Japan, 211(7), pp.576~582 (2006)
- Yi, J.M., "Acanthopanax senticosus root inhibits mast cell-dependent anaphylaxis", Clin. Chim. Acta, 312(1), pp.163~168 (2001)
- Yi, J.M., "Effect of Acanthopanax senticosus stem on mast cell-dependent anaphylaxis", J. Ethnopharmacol, 79(3), pp.347~352 (2002)