본문 바로가기
KERD
논문KCI 등재

Barley Malt Treated with Enzymes Increases Polyphenol Content and Antioxidant Activity

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

초록

The purpose of this study was to improve the functionality of a healthy drink with examining the possibility of manufacturing different enzymes (alpha-, beta-, glucose-amylase) in barley malts (BM) produced in various malting periods. The study showed that enzyme treatment increased significantly total polyphenol content (TPC), DPPH radical scavenging activity and hydroxly radical scavenging activity in malted liquid samples (MLS) which obtained from various malting periods. The highest of TPC were found in Gluco-24M with 1.981 ㎎TAE/㎖, followed by Beta-24M and Alpha-72M with 1.878 ㎎TAE/㎖ and 1.845 ㎎TAE/㎖, respectively. The DPPH result revealed that percent of inhibition increased by 71-75% compared to the control. No statistical difference was found between MLS obtained by 24 hr of malting (24 M) and 72 hr of malting (72 M) after enzyme treatment. In addition, an increasing of hydroxyl radical was in the same trend to the TPC and DPPH. The hydroxyl radical scavenging activity of enzyme treated samples was 1,5 times higher than the control. These results suggest the possibility of enzyme application to barley malts obtained in various germination periods for improving quality and functionality of barley malts.

Abstract

The purpose of this study was to improve the functionality of a healthy drink with examining the possibility of manufacturing different enzymes (alpha-, beta-, glucose-amylase) in barley malts (BM) produced in various malting periods. The study showed that enzyme treatment increased significantly total polyphenol content (TPC), DPPH radical scavenging activity and hydroxly radical scavenging activity in malted liquid samples (MLS) which obtained from various malting periods. The highest of TPC were found in Gluco-24M with 1.981 ㎎TAE/㎖, followed by Beta-24M and Alpha-72M with 1.878 ㎎TAE/㎖ and 1.845 ㎎TAE/㎖, respectively. The DPPH result revealed that percent of inhibition increased by 71-75% compared to the control. No statistical difference was found between MLS obtained by 24 hr of malting (24 M) and 72 hr of malting (72 M) after enzyme treatment. In addition, an increasing of hydroxyl radical was in the same trend to the TPC and DPPH. The hydroxyl radical scavenging activity of enzyme treated samples was 1,5 times higher than the control. These results suggest the possibility of enzyme application to barley malts obtained in various germination periods for improving quality and functionality of barley malts.

참고문헌 (20)

  1. Beta, T, "Phenolic content and antioxidant activity of pearled wheat fraction", Cereal Chemistry, 82(4), pp.390~393 (2005)
  2. Braca, A.G, "Antioxidant and free radical scavenging activity of flavonol glycosides from different Aconitum species", J. Ethnopharmacol, 86, pp.63~67 (2003)
  3. Bryjak, J, "Gluco-amylase, alpha-amylase and beta-amylase immobilisation on acrylic carriers", Biochemical Engineering Journal, 16, pp.347~355 (2003)
  4. 손영구, "보리저장밥법에 따른 주요 성분 및 발아력 변화", 한국작물학회지, 47(2), pp.201~205 (2002), 한국작물학회
  5. Chon, S.U, "Antioxidant activity of methanol extracts from root part of Korean salad plants", Korean J. Plant Res, 12(6), pp.413~419 (2008)
  6. Sang-Uk Chon, "Phytotoxicity and DPPH Radical Scavenging Activityof Barley Seedling Extracts", 한국작물학회지, 51(4), pp.322~328 (2006), 한국작물학회
  7. Cook, N.C, "Flavonoids-chemistry, metabolism, cardio protective effect, and dietary sources", Nutr. Biochem, 7, pp.66~76 (2006)
  8. Evers, A.D, "Scanning electron microscopy of wheat starch. II. Structure of granules modified by α-amylolysis-preliminary report", Staerke, 22, pp.23~ (1970)
  9. Fogarasi, A.L, "A comparative assessment of antioxidant properties, total phenolic content of einkorn, wheat, barley and their malts", Food Chem, 167, pp.1~6 (2015)
  10. 장현주, "제주 자생 식물들의 항산화 활성 능력 검색", 한국자원식물학회지, 28(2), pp.158~167 (2015), 한국자원식물학회[DOI]
  11. Halliwell, B, "Analytical Biochemistry", 165, pp.215~219 (1987)
  12. Laseken, O.O, "Effect of Vernonai amygdalina (bitter leaf) extract on brewing qualities and amino acid profiles of stout drinks from sorhum and barley malts", Food Chem, 64, pp.507~510 (1999)
  13. Lee,Y.T, "Quality characteristics of barley varieties related to enzymatic activity in malt", Korean J. Food Sci. Technol, 31(6), pp.1421~1426 (1999)
  14. 이나영, "Antioxidant Activity and Biological Activity of Specialty Barley (Hordeum vulgare L.) Extracts", 한국작물학회지, 55(2), pp.159~164 (2010), 한국작물학회
  15. Maillard, M.N, "Antioxidant activity of barley and malt: Relationship with phenolic content", Lebensmittel Wissenschaft and Technologie, 29, pp.238~244 (1996)
  16. Qingming, Y, "Antioxidant activity of malt extract from barley (Hordeum vulgare L.) toward various oxidative stress in vitro and in vivo", Food Chem, 188, pp.84~89 (2010)
  17. Su Noh Ryu, "Antioxidant Activity in Rice Cultivar , Wild Rice , and Barley", 한국작물학회지, 47(1), pp.54~61 (2002), 한국작물학회
  18. 석호문, "보리 도정부산물로부터 분리한 폴리페놀 추출물의 특성", 한국식품과학회지, 34(5), pp.775~779 (2002), 한국식품과학회
  19. Singleton, V.L, "Calorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents", American J. of Enology and Viticulture, 16, pp.144~158 (1965)
  20. Zielinski, H, "Antioxidant activity and total phenolics in selected cereal grains and their different morphological fraction", J. Agric. Food Chem, 48, pp.2008~2016 (2000)

관련 논문AI추천

이 간행물의 다른 논문 (11건)