한반도 자생식물 헐떡이풀 종자의 휴면유형과 발아특성
Seed Dormancy Type and Germination Characteristics in Tiarella polyphylla D. Don Native to Korea
2
조회
0
다운로드
9
인용
0
참고문헌
키워드
초록
헐떡이풀은 우리나라에서 울릉도에서만 분포하는 자생식물로 전통적으로 약용으로 쓰여왔으며, 관상식물로의 활용도 기대되는 자원식물이다. 본 연구는 헐떡이풀 종자의 휴면타파와발아를 위한 조건을 확립하고 종자휴면 유형을 분류하고자 수행되었다. 실험은 헐떡이풀 종자에 저온층적처리(5℃에서 0, 12주)와 고온층적처리(23℃에서 0, 4, 8, 12주 처리 후에 5℃에서 8주, 다시 23℃에서 배양함), GA3 (0, 10, 100, 1000 ㎎/L)처리를 수행하였다. 이렇게 처리한 종자는 무균배지에 소독하여파종하고 온도 23℃, 광도 40 μ㏖․m-2 ․ s-1 PPFD, 일장 16시간조건의 배양실에서 배양하였다. 헐떡이풀 종자는 자연상태에서 미숙배인 상태로 탈리되며, 종자 내부로의 수분흡수에 대한물리적인 장벽은 없었다. 그러나 아무 처리 없이 파종하였을 때30일이 지나도록 전혀 발아하지 않았다. 따라서 헐떡이풀 종자는 형태적 휴면(MD)과 생리적 휴면(PD)을 가지고 있는 것으로판단하였다. 저온층적처리(0, 12주) 실험의 최종 발아율은 각각66.7%, 45.6%로 나타났다. 저온처리는 오히려 발아를 약 3주 정도 지연시켰다. 고온층적처리(0, 4, 8, 12주) 실험에서는 최종발아율이 각각 13.3%, 24.4%, 20.0%, 51.1%로 나타났다. 헐떡이풀 종자의 배는 상대적 고온에서 발달하였다. GA3 처리는 종
Abstract
Tiarella polyphylla D. Don is a native plant distributed only in Ulleung Island in Korea and has been traditionally used for medicinal purposes, although it is also used ornamentally. This study was conducted to determine the requirements for dormancy break and germination and to classify the type of seed dormancy. The experiments were performed with cold stratification (0 or 12 weeks at 5℃), warm stratification (0, 4, 8, or 12 weeks at 23℃, followed by 8 weeks at 5℃, and then incubation at 23℃), and GA3 treatments (0, 10, 100, or 1000 ㎎/L). The treated seeds were incubated on aseptic media at room chamber (23℃, a 16h photoperiod of fluorescent lamps with 40 μmol ․ m-2 ․ s-1). The seeds were dispersed in nature as underdeveloped embryos with no physical barrier to absorb water to prevent water absorption. However, the seeds did not germinate for 30 days after sowing without any pre-treatments. Thus, the seeds had morphological dormancy (MD) and physiological dormancy (PD). The final germination percentage following cold stratification (0 or 12 weeks) was 66.7% and 45.6%, respectively. The cold stratification delayed seed germination by about 3 weeks. In the warm stratification experiment (0, 4, 8, or 12 weeks), the final germination percentage was 21.1%, 27.8%, 41.1%, and 57.8%, respectively, 20 weeks after sowing. The embryos of the T. polyphylla seed grew in relatively warm temperatures (23℃). GA3 application overcame seed dormancy and promoted germination. Following GA3 treatment (0, 10, 100, or 1000 ㎎/L), the final germination percentage was 33.3%, 45.0%, 42.5%, and 72.5%, respectively. These results suggest that the T. polyphylla seeds had non-deep simple morphophysiological dormancy (MPD) and GA3 treatment could be used as a substitute for warm stratification for breaking seed dormancy. To our knowledge, this is the first report of seed dormancy characteristics of the genus Tiarella native to Korea. Key words - Gibberellic acid, Morphophysiological dormancy, Non-deep simple MPD, Saxifragaceae, Warm stratification
참고문헌 (28)
- Baskin, C.C., "Germination ecophysiology of herbaceous plant species in a temperate region", Amer. J. Bot., 75, pp.286~305 (1988)
- Baskin, C.C., "A revision of Martin's seed classification system, with particular reference to his dwarf-seed type", Seed Sci. Res., 17, pp.11~20 (2007)
- Baskin, C.C., "Seeds: Ecology, Biogeography, Evolution of Dormancy and Germination" (2014), Academic Press
- Baskin, J.M., "A classification system for seed dormancy", Seed Sci. Res., 14, pp.1~16 (2004)
- Benedict, L., "Propagation protocol for black ash, Fraxinus nigra Marsh", Native Plants J., 3, pp.100~103 (2003)
- 조주성, "난쟁이바위솔의 종자발아를 촉진하는 온도, 광 및 화학적 처리", 화훼연구, 22(2), pp.54~59 (2014), 한국화훼학회[DOI]
- Finch-Savage, W.E., "Seed dormancy and the control of germination", New Phytologist, 171, pp.501~523 (2006)
- 장보국, "자생 숙은노루오줌의 종자발아에 미치는 환경조건과 화학적 처리의 영향", 한국자원식물학회지, 29(2), pp.235~240 (2016), 한국자원식물학회[DOI]
- Kitto, S.L., "Micropropagation and field establishment of Tiarella corditolia", J. Environ. Hort., 10, pp.171~174 (1992)
- Kullmann, W.H., "Seed Germination Records of Western Australian Plants. Kings Park Research Notes Number 7", ~ (1981)
- Lee, B.C., "Rare Plants Data Book in Korea", pp.234~ (2008), Korea National Arboretum
- Lee, M.Y., "Tiarellic acid attenuates airway hyperresponsiveness and inflammation in a murine model of allergic asthma", Intl. Immunopharmacol, 12, pp.117~124 (2012)
- Martin, A., "The comparative internal morphology of seeds", The Amer. Midl. Nat., 36, pp.513~660 (1946)
- Moon, H.I., "Triterpenoid from Tiarella polyphylla, regulation of type 1 procollagen and MMP-1 in ultraviolet irradiation of cultured old age human dermal fibroblasts", Arch. Pharm. Res., 27, pp.1060~1064 (2004)
- "NKISBS"[URL]
- Nikolaeva, M.G., "Pattern of seeds dormancy and germination as related to plant phylogeny and ecological and geographical conditions of their habitats", Russ. J. Plant Physiol., 46, pp.369~373 (1999)
- Ooi, M.K.J., "Dormancy and the firecentric focus: Germination of three Leucopogon species (Ericaceae) from south-eastern Australia", Ann. Bot., 98, pp.421~430 (2006)
- 박시형, "Structure Determination of a New Lupane-type Triterpene, Tiarellic Acid, Isolated from Tiarella polyphylla", Archives of Pharmacal Research, 25(1), pp.57~60 (2002), 대한약학회
- Park, S.H, "Anticomplement activities of oleanolic acid monodesmosides and bisdesmosides isolated from Tiarella polyphylla", Arch. Pharm. Res, 22, pp.428~437 (1999)
- Piotto, B., "Effects of temperature on germination of stratified seeds of three ash species", Seed Sci. Technol., 22, pp.519~529 (1994)
- "RHS Horticultural Database"[URL]
- Steinbauer, G.P., "Dormancy and germination of Fraxinus seeds", Plant Physiol., 12, pp.813~824 (1937)
- Taylor, J., "Propagation Successes, Failures and Lessons Learned;Proceedings of the Conference: Native Plant Propagation and Restoration Strategies", Proceedings of the Conference: Native Plant Propagation and Restoration Strategies, pp.45~54 (2001), Nursery Technology Cooperative, Oregon State University and Western Forestry and Conservation Association
- "The Plant List 2013"[URL]
- "USDA NRCS 2017"[URL]
- Vandelook, F., "Seed dormancy and germination of the European Chaerophyllum temulum (Apiaceae), a member of a trans-atlantic genus", Ann. Bot., 100, pp.233~239 (2007)
- Villiers, T.A., "Dormancy in fruits of Fraxinus excelsior L", J. Exp. Bot., 15, pp.359~367 (1964)
- Walck, J.F., "Seeds of Thalictrum merabile (Ranunculaceae) require cold stratification for loss of nondeep simple morphophysiological dormancy", Can. J. Bot., 77, pp.1769~1776 (1999)