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Optimization of Shoot Induction, Histological Study and Genetic Stability of in vitro Cultured Pisum sativum cv. ‘Sparkle’

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초록

An efficient shoot regeneration condition for pea cv. ‘Sparkle’ was developed by using optimum explant, plantgrowth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed thehighest on two kinds of shoot induction media (MSB5 media containing 2 ㎎/L BA and a combination of 2 ㎎/L BA and 1㎎/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 ㎎/L BA solutionwas found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division andproto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlierthan 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitroregenerated plants showed polymorphism with 8.3% compared with their mother plants.

Abstract

An efficient shoot regeneration condition for pea cv. ‘Sparkle’ was developed by using optimum explant, plantgrowth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed thehighest on two kinds of shoot induction media (MSB5 media containing 2 ㎎/L BA and a combination of 2 ㎎/L BA and 1㎎/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 ㎎/L BA solutionwas found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division andproto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlierthan 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitroregenerated plants showed polymorphism with 8.3% compared with their mother plants.

참고문헌 (37)

  1. Mitra Aftabi, "Improved Protocol for Agrobacterium-Mediated Transformation of Pea (Pisum sativum)", Molecular Biology, 7(1) (2017), OMICS Publishing Group[DOI]
  2. Ai-Qurainy, F., "Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia", Saudi J. Biol. Sci, 25, pp.111~116 (2018)
  3. Azade, L. K., "In vitro regeneration of pigeon pea using leaf explants", Afr. Crop Sci. J, 24(2), pp.191~201 (2016)
  4. Benson, E. E, "Special symposium: In vitro plant recalcitrance:An introduction", In Vitro Cell Dev. Biol. Plant, 36, pp.141~148 (2000)
  5. Bohmer, P., "Thidiazuron induced high-frequency of shoot induction and plant regeneration in protoplast derived pea callus", Plant Cell Rep, 15(1-2), pp.26~29 (1995)
  6. Brown, D.C.W., "Cell Culture and Somatic Cell Genetics of Plants", pp.49~65 (1986), Academic Press
  7. Cao, X., "A two-step pretreatment significantly enchance shoot organogenesis from leaf explant from highbush blueberry cultivar bluecrop", Hort. Sci, 37(5), pp.819~821 (2002)
  8. Dibax, R., "Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars, Ciência Rural", Santa Maria, 43(1), pp.49~54 (2013)
  9. Fatima, N., "Role of growth regulators on in vitro regeneration and histological analysis in Indian ginseng(Withania somnifera L. )Dunal", Physiol. Mol. Biol. Plants, 18(1), pp.59~67 (2012)
  10. Goto, S., "Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers", Plant Cell Rep, 18, pp.193~197 (1998)
  11. Huang, W. J., "Determination of genetic stability of long-term micropropagated plantlets of Platanus acerifolia using ISSR markers", Biol. Plant, 53(1), pp.159~163 (2009)
  12. Jackson, J. A., "Rapid multiple shoot production from cotyledonary node explants of pea(Pisum sativum L. )", In Vitro Cell Dev. Biol. Plant, 26, pp.825~835 (1990)
  13. Kantayos, V., "Characterization of plantlet regeneration and genetic transformation of legume plants", Ph.D., pp.2019
  14. 김동건, "기내 배양을 통한 국내 콩(Glycine max L.) 품종의 식물체 재분화", 한국자원식물학회지, 29(1), pp.143~153 (2016), 한국자원식물학회[DOI]
  15. Klu, G. Y. P., "Efforts to accelerate domestication of winged bean (Psophocarpus tetragonolobus (L.) DC.) by means of induced mutations and tissue culture", Department Laboratory of Plant Breeding, pp.1996
  16. Mallo’n, R., "In vitro propagation of the endangered plant Centaure aultreiae : assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis", Plant Cell Tiss. Org. Cult, 101, pp.31~39 (2010)
  17. Mariappan, M., "Organogenesis and evaluation of genetic homogeneity through SCoT and ISSR markers in Heliteres idora L., a medicinally important tree", S. Afr. J. Bot., 106, pp.204~210 (2016)
  18. Murashige, T., "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol. Plant, 15, pp.473~497 (1962)
  19. Nayak, S., "In vitro and ex vitro evaluation of long-term microparpagated turmeric as analyzed through cytophotometry, phytoconsituents, biochemical and molecular markers", Plant Growth Regul, 64, pp.91~98 (2011)
  20. Nayak, S. A., "In vitro plant regeneration from cotyledonary nodes of Withania somnifera(L. )Dunal and assessment of clonal fidelity using RAPD and ISSR markers", Acta Physiol. Plant, 35(1), pp.195~203 (2012)
  21. Raveendar, S., "Development of a rapid, highly efficient system of organogenesis in cowpea Vigna unguiculata(L. )Walp", S. Afri. J. Bot., 75, pp.17~21 (2009)
  22. Reinhardt, D., "Regulation of phyllotaxis by polar auxin transport", Nature, 426, pp.255~260 (2003)
  23. Sainger, M., "Development of an efficient in vitro plant regeneration system amenable to Agrobacterium-mediated transformation of a recalcitrant grain legume blackgram(Vigna mungo L. Hepper)", Physiol. Mol. Biol. Plants, 21(4), pp.505~517 (2015)
  24. Schroeder, H. E., "Transformation and regeneration of two cultivar of pea(Pisum sativum)", Mol. Biol. Gene Reg., 101, pp.751~757 (1993)
  25. Shah, S. H., "Callus induction, in vitro shoot regeneration and hairy root formation by the the assessment of various plant growth regulators in tomato(Solanum lycopersicum Mill. )", J. Ani. Plant Sci, 25(2), pp.528~538 (2015)
  26. Swartz, H. J., "The effect of in vitro pretreatments on subsequent shoot organogenesis from exicised Rubus and Malus leaves", Plant Cell Tiss. Org. Cult., 21, pp.179~184 (1990)
  27. Thomas, T. D, "Pretreatment in thidiazuron improves the in vitro shoot induction from leaves in Curculigo orchioides Gaertn., an endangered medicinal plant", Acta Physiol. Plant, 29, pp.455~461 (2007)
  28. Tie, M., "Effect of 6-BA on the plant regeneration via organogenesis from cotyledonary node of cowpea (Vigna unguiculata L. Walp)", J. Agr. Sci, 5(5) (2013)[DOI]
  29. Tran, T. H., "Role of plant growth regulators on shoot development of shoot apical meristems of banana genotypes", Acta Hort., 1114, pp.211~218 (2016)
  30. Tzitzikas, E. N., "Regeneration of pea(Pisum sativum L. )by a cyclic organogenic system", Plant Cell Rep, 23, pp.453~460 (2004)
  31. Veltcheva, M., "In vitro cultivation and regeneration of bean(Phaseolus vulgaris L. )", Biotechnol. Biotechnol. Equip, 17(1), pp.50~58 (2003)
  32. Wang, H., "Histological and endogenous plant growth regulators changes associated with adventitious shoot regeneration from in vitro leaf explants of strawberry(Fragaria × ananassa cv. ‘Honeoye’)", Plant Cell Tiss. Organ Cult, 23(3), pp.479~488 (2015)
  33. Werner, E. T., "Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers", Gene Mol. Res, 14(4), pp.16450~16460 (2015)
  34. Woo, S. M., "Morphological and histological evaluations of in vitro regeneration in Elliottia racemosa leaf explants induced on media with thidiazuron", J. Am. Soc. Hort. Sci., 133(2), pp.167~172 (2008)
  35. Yancheva, S., "Regeneration possibilities of Bulgarian bean cultivars Plovdiv 11M and Dobrudjanski 7", Biotechnol. Biotechnol. Equip, 13(1), pp.40~43 (1999)
  36. 이정윤, "Cryopreservation of Citrus limon (L.) Burm. F Shoot Tips", 한국자원식물학회지, 31(6), pp.684~694 (2018), 한국자원식물학회[DOI]
  37. Zhihui, S., "Effect of TDZ on plant regeneration from mature seeds in pea(Pisum sativum)", In Vitro Cell Dev. Biol. Plant, 45(6), pp.776~782 (2009)

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