Optimization of Shoot Induction, Histological Study and Genetic Stability of in vitro Cultured Pisum sativum cv. ‘Sparkle’
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초록
An efficient shoot regeneration condition for pea cv. ‘Sparkle’ was developed by using optimum explant, plantgrowth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed thehighest on two kinds of shoot induction media (MSB5 media containing 2 ㎎/L BA and a combination of 2 ㎎/L BA and 1㎎/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 ㎎/L BA solutionwas found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division andproto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlierthan 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitroregenerated plants showed polymorphism with 8.3% compared with their mother plants.
Abstract
An efficient shoot regeneration condition for pea cv. ‘Sparkle’ was developed by using optimum explant, plantgrowth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed thehighest on two kinds of shoot induction media (MSB5 media containing 2 ㎎/L BA and a combination of 2 ㎎/L BA and 1㎎/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 ㎎/L BA solutionwas found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division andproto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlierthan 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitroregenerated plants showed polymorphism with 8.3% compared with their mother plants.
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