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댕댕이나무(Lonicera caerulea)의 대장암세포 생육억제 활성

Inhibitory Activity of Lonicera caerulea Against Cell Proliferation in Human Colorectal Cancer Cells

박광훈|손호준|정진부|어현지|안미연
한국자원식물학회 (The Plant Resources Society Of Korea)|2020.08|한국자원식물학회지 (Korean Journal of Plant Reources.)|제33권 제4호|pp. 263-270

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참고문헌

키워드

β-CateninAnticancer activityTCF4Lonicera caerulea

초록

이상의 연구 결과로 미루어 볼 때, 댕댕이나무 잎과 가지 추추출물은 대장암 세포주 HCT116과 SW480세포의 생육을 억제하였으나 열매추출물은 억제활성이 나타나지 않았다. 잎과 가지 추출물은 cell migration과 wound healing assay를 통해 비정상적인 세포증식 억제를 확인하였으며, β-catenin과 TCF4 의 단백질 수준을 감소시켜 비정상적인 Wnt 신호전달을 억제를통해 대장암세포의 생육을 억제하는 것으로 판단된다. 따라서댕댕이나무 잎과 가지는 항암을 위한 대체보완소재 및 천연 항암제 개발을 위한 소재로 활용이 가능할 것으로 판단된다.

Abstract

In this study, we evaluated the effect of the extracts from Lonicera caerulea leaves (LCLE), branches (LCBE) and fruits (LCFE) on the cell growth and migration in human colorectal cancer cells, HCT116 and SW480 cells. LCLE and LCBE dose- and time-dependently inhibited the proliferation of HCT116 and SW480 cells. However, LCFE did not affect the proliferation of HCT116 and SW480 cells. In addition, LCLE and LCBE dramatically cell migration and wound healing in HCT116 cells. LCLE and LCBE decreased β-catenin protein level but not mRNA level in HCT116 and SW480 cells. Furthermore, LCLE decreased TCF4 level in both protein and mRNA level in HCT116 and SW480 cells. However, LCBE decreased TCF4 protein level but not mRNA level in HCT116 and SW480 cells. Based on these findings, LCLE and LCBE may inhibit the cell proliferation and migration through blocking Wnt signaling activation in human colorectal cancer cells. Therefore, LCLE and LCBE may be a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer.

참고문헌 (35)

  1. Aggarwal, B. B., "From traditional Ayurvedic medicine to modern medicine: identification of therapeutic targets for suppression of inflammation and cancer", Expert Opin. Ther. Targets, 10, pp.87~118 (2006)
  2. Center, M. M., "Ward. Worldwide variations in colorectan cancer", CA Cancer J. Clin, 59, pp.366~378 (2009)
  3. Chambers, A. F., "Dissemination and growth of cancer cells in metastatic sites", Nat. Rev. Cancer, 2, pp.563~572 (2002)
  4. Chen, L., "Phytochemical properties and antioxidant capacities of various colored berries", J. Sci. Food Agric, 94, pp.180~188 (2014)
  5. Friedl, P., "Tumour-cell invasion and migration:Diversity and escape mechanisms", Nat. Rev. Cancer, 3, pp.362~374 (2003)
  6. Friedl, P., "Cancer invasion and the microenvironment : plasticity and reciprocity", Cell, 147, pp.992~1009 (2011)
  7. Ghadimi, B. M., "Effectiveness of gene expression profiling for response prediction of rectal adenocarcinomas to preoperative chemoradiotherapy", J. Clin. Oncol, 23, pp.1826~1836 (2016)
  8. He, T. C., "Identification of c-MYC as a target of the APC pathway", Science, 281, pp.1509~1512 (1998)
  9. Hrckulak, D., "Wnt Effector TCF4 Is Dispensable for Wnt Signaling in Human Cancer Cells", Genes, 9, pp.439~ (2018)
  10. Hsiao, W. L. W., "The role of traditional Chinese herbal medicines in cancer therapy from TCM theory to mechanistic insights", Planta Med, 76, pp.1118~1131 (2010)
  11. Iwai, S., "Involvement of the Wnt-β-catenin pathway in invasion and migration of oral squamous carcinoma cells", Int. J. Oncol, 37, pp.1095~1103 (2010)
  12. Jin, X. H., "Effects of blue honeysuckle (Lonicera caerulea L.) extract on lipopolysaccharide-induced inflammation in vitro and in vivo", Exp. Eye Res, 82, pp.860~867 (2006)
  13. Jones, D. H., "Regulation of cancer cell migration and bone metastasis by RANKL", Nature, 440, pp.692~696 (2006)
  14. 정규원, "Prediction of Cancer Incidence and Mortality in Korea, 2019", Cancer Research and Treatment, 51(2), pp.431~437 (2019), 대한암학회
  15. Jurgonski, A., "An anthocyanin-rich extract from Kamchatka honeysuckle increases enzymatic activity within the gut and ameliorates abnormal lipid and glucose metabolism in rats", Nutrition, 29, pp.898~902 (2013)
  16. Kikuchi, H., "Chemopreventive and anticancer activity of flavonoids and its possibility for clinical use by combining with conventional chemotherapeutic agents", Am. J. Cancer Res., 9, pp.1517~1535 (2019)
  17. 김하나, "도깨비부채(Rodgersia podophylla) 잎 추출물의 인간 암세포의 β-catenin 분해 유도 활성", 한국자원식물학회지, 32(5), pp.442~447 (2019), 한국자원식물학회[DOI]
  18. Kim, J. W., "Anti-obesity and fatty liver-preventing activities of Lonicera caerulea in high-fat diet-fed mice", Int. J. Mol. Med, 42, pp.3047~3064 (2018)
  19. 이유숙, "Evaluation of in vitro anti-oxidant and anti-inflammatory activities of Korean and Chinese Lonicera caerulea", Nutrition Research and Practice, 12(6), pp.486~493 (2018), 한국영양학회
  20. Neth, P., "The Wnt signal transduction pathway in stem cells and cancer cells: influence on cellular invasion", Stem Cell Rev, 3, pp.18~29 (2007)
  21. Neth, P., "Wnt signaling regulates the invasion capacity of human mesenchymal stem cells", Stem Cells, 24, pp.1892~1903 (2006)
  22. Newman, D. J., "Natural products as sources of new drugs over the last 25 years", J. Nat. Prod, 70, pp.461~477 (2007)
  23. Pace, E., "Impact of thermal degradation of cyanidin-3-O-glucoside of haskap Berry on cytotoxicity of hepatocellular carcinoma HepG2 and breast cancer MDA-MB-231 cells", Antioxidants (Basel), 7, pp.E24~ (2018)
  24. Palikova, I., "Protectivity of blue honeysuckle extract against oxidative human endothelial cells and rat hepatocyte damage", J. Agric. Food Chem, 57, pp.6584~6589 (2009)
  25. Park, S. I., "Therapeutic effects of blue honeysuckle on lesions of hyperthyroidism in rats", Am. J. Chin. Med, 44, pp.1441~1456 (2016)
  26. Park, Y., "Dietary patterns and colorectal cancer risk in a Korean population", Medicine (Baltimore), 95, pp.E3759~ (2016)
  27. Plekhanova, M. N, "Blue Haneysuckle (Lonicera caerulea L.)-A new commercial berry crop for temperate climate:Genetic resources and breeding", Acta Hort, 538, pp.159~ (2000)
  28. Smolik, M., "Genetic variability of Polish and Russian accessions of cultivated blue honeysuckle (Lonicera caerulea)", Russ. J. Genet, 46, pp.1079~ (2010)
  29. Svarcova, I., "Berry fruits as a source of biologically active compounds: The case of Lonicera caerulea", Biomed. Pap. Med. Fac. Univ. Palacky Olomouc. Czech, 151, pp.163~174 (2017)
  30. Szopa, A., "Comparative analysis of different groups of phenolic compounds in fruit and leaf extracts of Aronia sp.:A. melanocarpa, A. arbutifolia, and A.×prunifolia and their antioxidant activities", Eur. Food Res. Technol, 243, pp.1645~1657 (2017)
  31. H. Ekiert, "Comparative analysis of different groups of phenolic compounds in fruit and leaf extracts of Aronia sp.:A. melanocarpa, A. arbutifolia, and A.×prunifolia and their antioxidant activities", Eur. Food Res. Technol, 243, pp.1645~1657 (2017)
  32. Tetsu, O., "Beta-catenin regulates expression of cyclin D1 in colon carcinoma cells", Nature, 398, pp.422~426 (1999)
  33. Yang, C., "β-Catenin promotes cell proliferation, migration, and invasion but induces apoptosis in renal cell carcinoma", Onco. Targets Ther., 10, pp.711~724 (2017)
  34. Zdarilova, A., "Polyphenolic fraction of Lonicera caerulea L. fruits reduces oxidative stress and inflammatory markers induced by lipopolysaccharide in gingival fibroblasts", Food Chem. Toxicol, 48, pp.1555~1561 (2010)
  35. Zhou, L., "Anti-tumor properties of anthocyanins from Lonicera caerulea ‘Beilei’ fruit on human hepatocellular carcinoma: In vitro and in vivo study", Biomed. Pharmacother, 104, pp.520~529 (2018)

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