Investigation of Defense and Vegetative Growth Related Traits of Recombinant Inbred Lines of Brassica rapa
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Brassica rapa is one of the most valuable vegetable crops worldwide. Cultivated varieties of B. rapa exhibitdiverse developmental and morphological appearances, which includes important vegetables, oilseeds, and fodder crops. Inthis study, various phenotypes of recombinant inbred lines (RILs) of B. rapa were investigated, including their responses tofive different pathogenic Botrytis cinerea isolates, responses to aphid and thrips during flowering stages, days to flowering,and plant heights. Responses of 113 RILs to five different B. cinerea isolates showed variations, suggesting that geneticfactors controlling resistance or tolerance against each isolate were dependent on isolate/genotype pairs. Correlationanalysis was performed to understand the nature of genetic factors and the relationship among these phenotypes. Althoughhigh levels of correlation were not detected between phenotypes assessed in this study, statistically significant correlationwas detected for several combinations. Significant positive correlations were found for different B. cinerea isolates,supporting that certain levels of commonality could exist in genetic components controlling resistance against different B. cinerea isolates. Based on correlation analysis using numbers of insects counted on plants, it was speculated that geneticfactors responsible for aphid tolerance or repellence might be also involved in the response against thrips. Relationshipbetween vegetative growth and tolerance against B. cinereal or insects is rather more complicated. However, it was observedthat shorter plants appeared to have a certain level of tolerance or repellence against both aphids and thrips. Data presentedin this study could be used to assist further genetic studies and breeding efforts to obtain Botritis and insect resistance for B. rapa.
Abstract
Brassica rapa is one of the most valuable vegetable crops worldwide. Cultivated varieties of B. rapa exhibitdiverse developmental and morphological appearances, which includes important vegetables, oilseeds, and fodder crops. Inthis study, various phenotypes of recombinant inbred lines (RILs) of B. rapa were investigated, including their responses tofive different pathogenic Botrytis cinerea isolates, responses to aphid and thrips during flowering stages, days to flowering,and plant heights. Responses of 113 RILs to five different B. cinerea isolates showed variations, suggesting that geneticfactors controlling resistance or tolerance against each isolate were dependent on isolate/genotype pairs. Correlationanalysis was performed to understand the nature of genetic factors and the relationship among these phenotypes. Althoughhigh levels of correlation were not detected between phenotypes assessed in this study, statistically significant correlationwas detected for several combinations. Significant positive correlations were found for different B. cinerea isolates,supporting that certain levels of commonality could exist in genetic components controlling resistance against different B. cinerea isolates. Based on correlation analysis using numbers of insects counted on plants, it was speculated that geneticfactors responsible for aphid tolerance or repellence might be also involved in the response against thrips. Relationshipbetween vegetative growth and tolerance against B. cinereal or insects is rather more complicated. However, it was observedthat shorter plants appeared to have a certain level of tolerance or repellence against both aphids and thrips. Data presentedin this study could be used to assist further genetic studies and breeding efforts to obtain Botritis and insect resistance for B. rapa.
참고문헌 (42)
- Amasino, R. M, "Control of flowering time in plants", Curr. Opin. Genet. Dev, 6, pp.480~487 (1996)
- Amselem, J., "Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea", PLoS Genet, 7, pp.e1002230~ (2011)
- Bhatia, V., "Aphid resistance in Brassica crops: Challenges, biotechnological progress and emerging possibilities", Biotechnol. Adv., 29, pp.879~888 (2011)
- Bloomer, R.H., "Fine-tuning timing: Natural variation informs the mechanistic basis of the switch to flowering in Arabidopsis thaliana", J. Exp. Bot., 68, pp.5439~5452 (2017)
- Bolton, M. D., "Sclerotinia sclerotiorum (Lib.) de bary: Biology and molecular traits of a cosmopolitan pathogen", Mol. Plant Pathol, 7, pp.1~16 (2006)
- Bragard, C., "Status and prospects of plant virus control through interference with vector transmission", Annu. Review Phytopathol., 51, pp.177~201 (2013)
- Campos, M. L., "Rewiring of jasmonate and phytochrome B signalling uncouples plant growth-defense tradeoffs", Nat. Commun, 7, pp.12570~ (2016)
- Chouard, P, "Vernalization and its relations to dormancy", Annu. Rev. Plant Physiol, 11, pp.191~238 (1960)
- Denby, K. J., "Identifi cation of Botrytis cinerea susceptibility loci in Arabidopsis thaliana", Plant J, 38, pp.473~486 (2004)
- De Vos, M., "Biochemistry and molecular biology of Arabidopsis -aphid interactions", Bio Essays, 29, pp.871~883 (2007)
- Diaz-Montano, J., "Onion thrips (Thysanoptera: Thripidae): a global pest of increasing concern in onion", J. Econ. Entomol, 104, pp.1~13 (2011)
- FitzJohn, R. G., "Hybridisation within Brassica and allied genera: evaluation of potential for transgene escape", Euphytica, 158, pp.209~230 (2007)
- Goggin, F. L, "Plant-aphid interactions: molecular and ecological perspectives", Curr. Opin. Plant Biology, 10, pp.399~408 (2007)
- Guo, Y., "Center of origin and centers of diversity in an ancient crop", Brassica rapa (turnip rape) J. Hered., 5, pp.555~565 (2014)
- Hogenhout, S. A., "Insect vector interactions with persistently transmitted viruses", Annu. Rev. Phytopathol, 46, pp.327~359 (2008)
- Hopkins, R. J., "Role of glucosinolates in insect-plant relationships and multitrophic interactions", Annu. Rev. Entomol, 54, pp.57~83 (2009)
- Howe, G. A., "Plant immunity to insect herbivores", Annu. Rev. Plant Biol, 59, pp.41~66 (2008)
- Huot, B., "Growthdefense tradeoffs in plants: a balancing act to optimize fitness", Mol. Plant, 7, pp.1267~1287 (2014)
- Iniguez-Luy, F. L., "Development of public immortal mapping populations, molecular markers and linkage maps for rapid cycling Brassica rapa and B. oleracea", Theor. Appl. Genet, 120, pp.31~43 (2009)
- Jones, A. M. E., "Purification and characterisation of a nonplant myrosinase from the cabbage aphid Brevicoryne brassicae (L.)", Insect Biochem. Mol. Biol, 31, pp.1~5 (2001)
- Karasov, T. L., "Mechanisms to mitigate the trade-off between growth and defense", Plant Cell, 29, pp.666~680 (2017)
- 김진희, "Development of a High-throughput SNP Marker set by Transcriptome Sequencing to Accelerate Genetic Background Selection in Brassica rapa", Horticulture, Environment, and Biotechnology, 57(3), pp.280~290 (2016), 한국원예학회[DOI]
- Kliebenstein, D. J., "Secondary metabolites influence Arabidopsis", Botrytis interactions : Variation in host production and pathogen sensitivity, 2005, pp.44, 25~36
- Koornneef, M., "Naturally occurring genetic variation in Arabidopsis thaliana Annu", Rev. Plant. Biol., 55, pp.141~172 (2004)
- Li, X., "Construction of chromosome segment substitution lines enables QTL mapping for flowering and morphological traits in Brassica rapa", Front. Plant Sci, 6, pp.432~ (2015)
- Lou, P., "Quantitative trait loci for flowering time and morphological traits in multiple populations of Brassica rapa", J. Exp. Bot., 58, pp.4005~4016 (2007)
- Mason, A. S., "Oilseed rape: Learning about ancient and recent polyploid evolution from a recent crop species", Plant Biol, 18, pp.883~892 (2016)
- Mitchell, C., "Plant defense against herbivorous pests: Exploiting resistance and tolerance traits for sustainable crop protection", Front. Plant Sci, 7, pp.1132~ (2016)
- Powell, G., "Host plant selection by aphids: Behavioral, evolutionary, and applied perspectives", Annu. Rev. Entomol., 51, pp.309~330 (2005)
- Roux, F., "How to be early flowering: an evolutionary perspective", Trends Plant Sci, 11, pp.375~381 (2006)
- Rowe, H. C., "Complex genetics control natural variation in Arabidopsis thaliana resistance to Botrytis cinerea", Genetics, 180, pp.2237~2250 (2008)
- Shea, D. J., "The role of FLOWERING LOCUS C in vernalization of Brassica: The importance of vernalization research in the face of climate change", Crop Pasture Sci., 69, pp.30~39 (2017)
- Song, Y. H., "CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis", Plant J., 69, pp.332~342 (2012)
- Staal, J., "RLM3, a TIR domain encoding gene involved in broad-range immunity of Arabidopsis to necrotrophic fungal pathogens", Plant J., 55, pp.188~200 (2008)
- Staats, M., "Genome update of Botrytis cinerea strains B05.10 and T4", Eukaryot. Cell, 11, pp.1413~1414 (2012)
- Vanous, A., "Association mapping of flowering and height traits in germplasm enhancement of maize doubled haploid lines", Plant Genome, 11(2), pp.1~14 (2018)
- van Wees, S, "Phenotypic analysis of Arabidopsis mutants:Trypan blue stain for fungi, oomycetes, and dead plant cells", CSH Protoc, 2008(8), pp.1101~ (2008)[DOI]
- Williams, P.H., "Rapid-cycling populations of brassica", Science, 232, pp.1385~1389 (1986)
- Williamson, B., "Botrytis cinerea: the cause of grey mould disease", Mol. Plant Pathol., 8, pp.561~580 (2007)
- Zhang, W., "Isolate dependency of Brassica rapa resistance QTLs to Botrytis cinerea", Front. Plant Sci., 7, pp.161~ (2016)
- Zhao, J., "Genetic relationships within Brassica rapa as inferred from AFLP fingerprints", Theor. Appl. Genet., 110, pp.1301~1314 (2005)
- Züst, T., "Trade-offs between plant growth and defense against insect herbivory: An emerging mechanistic synthesis", Annu. Rev. Plant Biol, 68, pp.513~534 (2017)