내부기생성선충 Pratylenchus vulnus 분리 효율 비교
Comparison of Extraction Efficacy for Endoparasitic Nematodes Pratylenchus vulnus from Roots
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초록
식물 뿌리로부터 내부기생성 선충을 단기간에 효율적으로분리하기 위하여 1) 뿌리 침지 분리, 2) 뿌리 침지+산소공급 분리, 3) 오스텐접시 분리, 4) 미스트장치 등 4가지 분리방법과 뿌리 파쇄법을 접목하여 상호 비교하였다. 시험은 뿌리썩이선충(Pratylenchus vulnus)에 감염된 들깨뿌리를 이용하였고, 9일간 선충을 분리하였다. 뿌리썩이선충은 들깨 뿌리로부터 9일까지 계속 분리되었으며, 9일째에도 전체 선충의 3~10%가 분리되었다. 들깨 뿌리 2 g에서 9일간 분리된 뿌리썩이선충 최종 밀도는 379~1,824 마리로 처리 간에 큰 차이가 있었는데, 선충이 많이 분리된 처리는 뿌리 파쇄+침지+공기주입(1,824마리) 처리와 뿌리 파쇄+미스트장치(1,349 마리)였다(p = 0.05). 2일째까지 뿌리썩이선충이 가장 많이 분리된 처리는 뿌리파쇄+미스트장치(725 마리), 다음으로 뿌리파쇄+침지+산소공급(555 마리), 뿌리파쇄+오스텐접시법(421 마리)이었다. 뿌리를 파쇄한후 분리한 방법은 파쇄하지 않고 분리한 방법보다 모든 분리방법에서 16~108%까지 더 많은 수의 선충이 분리되었다(p = 0.01).
Abstract
To speed up the extract of endoparasitic nematodes from roots, four extraction methods with or without root-grinding were compared; 1) immersion, 2) immersion + Air, 3) Oostenbrink dish, and 4) Mistifier. The experiments were conducted for nine days by using perilla roots infested with Pratylenchus vulnus. Root-lesion nematodes continuously extracted from perilla roots during the experiments and as much as 3-10% in 9th days. The total number of nematodes extracted from 2 g of perilla root in nine days were varied among methods (379-1,824 nematodes); the most nematodes were extracted by root grinding + immersion + air (1,824) and the root-grinding + mistifier method (1,349) (p = 0.05). In the first two days of extraction, root-grinding + mistifier extracted the most nematodes (725 nematodes), followed by root-grinding + immersion + Air (555 nematodes), and root-grinding + Oostenbrink dish (421 nematodes). Root-grinding effected as much as 16-108% more nematodes extraction when compared to without root-grinding (p = 0.01).
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