흰쥐의 양지(TE4)에 대한 신경해부학적 연구
Neuroanatomical Studies on Yangji(TE4) in the Rats
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This research was practiced to comparative investigate the distribution of sensory and motor neuron linkaged with Yangji(TE4) by using neural-tracer technology. A total 16 S-D rats were used in the present research. After anesthesia, the rats received micro-injection of 6㎕ of cholera toxin B subunit(CTB) into the relation positions of the Yangji(TE4), in the human body for observing the distribution of the linkaged sensory neurons in dorsal root ganglia(DRGs) and motor neurons in the spinal cord(C3∼T4) and sympathetic ganglia. 3 days after the micro injection, the rats were anesthetized and transcardially perfused saline and 4% paraformaldehyde, followed by routine section of the DRGs, sympathetic chain ganglia(SCGs) and spinal cord. Marked neurons and nerve fibers were detected by immunohistochemical method and observed by light microscope. The marked neurons were recorded and counted. From this study the distribution of primary sensory and motor neurons linkaged with Yangji(TE4) were concluded as follows. Yangji(TE4) dominated by spinal segments of C5∼T1, C6∼T4, individually.
Abstract
This research was practiced to comparative investigate the distribution of sensory and motor neuron linkaged with Yangji(TE4) by using neural-tracer technology. A total 16 S-D rats were used in the present research. After anesthesia, the rats received micro-injection of 6㎕ of cholera toxin B subunit(CTB) into the relation positions of the Yangji(TE4), in the human body for observing the distribution of the linkaged sensory neurons in dorsal root ganglia(DRGs) and motor neurons in the spinal cord(C3∼T4) and sympathetic ganglia. 3 days after the micro injection, the rats were anesthetized and transcardially perfused saline and 4% paraformaldehyde, followed by routine section of the DRGs, sympathetic chain ganglia(SCGs) and spinal cord. Marked neurons and nerve fibers were detected by immunohistochemical method and observed by light microscope. The marked neurons were recorded and counted. From this study the distribution of primary sensory and motor neurons linkaged with Yangji(TE4) were concluded as follows. Yangji(TE4) dominated by spinal segments of C5∼T1, C6∼T4, individually.
참고문헌 (21)
- Hwang YS, "Academic history of Chinese acupuncture", pp.595~607 (2005), pubinmunhwasa
- Zhao ZQ, "Neural mechanism underlying acupuncture analgesia", Prog Neurobiol, 85(4), pp.355~375 (2008)[DOI]
- Ma SX, "Neurobiology of acupuncture: toward CAM", Evid Based Complement Alternat Med, 1(1), pp.41~47 (2004)[DOI]
- Ha LJ, "The expression of calcitonin gene-related peptide in the sensory and motor neurons associated with "Hegu" (LI 4) in the rat", Zhen Ci Yan Jiu, 39(2), pp.112~116 (2014)
- Zhu XL, "Neuroanatomical characteristics of acupoint "Chengshan"(BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study", Zhen Ci Yan Jiu, 35(6), pp.433~437 (2010)
- Cui JJ, "Specificity of sensory and motor neurons associated with BL40 and GB30 in the rat: a dual fluorescent labeling study", Evid Based Complement Alternat Med, 2013, pp.1~6 (2013)
- Wang HF, "Retrograde and transganglionic transport of horseradish peroxidase-conjugated cholera toxin B subunit, wheatgerm agglutinin and isolectin B4 from Griffonia simplicifolia I in primary afferent neurons innervating the rat urinary bladder", Neuroscience, 87(1), pp.275~288 (1998)[DOI]
- Cui JJ, "Neuroanatomical basis for acupuncture point PC8 in the rat: neural tracing study with cholera toxin subunit B", Acupunct Med, 31(4), pp.389~394 (2013)[DOI]
- Jang I, "A study on the central neural pathway of the heart, Nei-Kuan (EH-6) and Shen-Men (He-7) with neural tracer in rats", Am J Chin Med, 31(4), pp.591~609 (2003)[DOI]
- Hirakawa M, "Time-related changes in the labeling pattern of motor and sensory neurons innervating the gastrocnemius muscle, as revealed by the retrograde transport of the cholera toxin B subunit", Cell tissue Res, 267(3), pp.419~427 (1992)[DOI]
- Cui JJ, "Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594", Zhen Ci Yan Jiu, 36(4), pp.262~267 (2011)
- Loewy AD, "CNS cell groups projecting to pancreatic parasympathetic preganglionic neurons", Brain Res, 620(2), pp.323~330 (1993)[DOI]
- Joosten EA, "Postnatal development of the ipsilateral corticospinal component in rat spinal cord : a light and electron microscopic anterograde HRP study", J Comp Neurol, 326(1), pp.133~146 (1992)[DOI]
- Strack AM, "Pseudorabies virus : a highly specific transneuronal cell body marker in the sympathetic nervous system", J Neurosci, 10(7), pp.2139~2147 (1990)[DOI]
- Cabot JB, "Descending Pathways to the Spinal Cord, Progress in Brain Research", pp.79~108 (1982), Elsevier Bionedical Press
- Brodal P, "Is lectin coupled horseradish peroxidase taken up and transported by damaged as well as by undamaged fibers in the centra nervous system", Brain Res, 278(1-2), pp.1~9 (1983)[DOI]
- Holstege JC, "Brainstem projection to spinal motoneurons: an update", Neuroscience, 23(3), pp.809~821 (1987)[DOI]
- Hsu SM, "Use of avidin-biotinperoxidase complex(ABC) in immunoperoxidase techniques : a comparison between ABC and unlabeled antibody(PAP) procedures", J Histochem Cytochem, 29(4), pp.577~580 (1981)[DOI]
- Card JP, "Pseudorabies virus infection of the rat central nervous system : ultrastructural characterization of viral replication, transport, and pathogenesis", J Neurosci, 13(6), pp.2515~2539 (1993)[DOI]
- Lee SR, "Acupointology", pp.587~589 (2007), Chunghong
- Woolf CJ, "The properties of neurons recorded in the superficial dorsal horn of the rat spinal cord", J Comp Neurol, 221(3), pp.313~328 (1983)[DOI]