足三里 전침자극에 의한 흰쥐 hypothalamus의 유전자 발현 profile 분석
Gene Expression Profile of Rat Hypothalamus Treated with Electroacupuncture at ST36 Acupoint
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Abstract
Electroacupuncture (EA) has been reported to increase pain threshold, and to enhance the NK cell activity by up-regulation of IFN-γ and endogenous β-endolphin. For the purpose of understanding the molecular mechanism of EA stimulation, we analyzed the gene expression profile of rat hypothalamus, treated on Zusanli (ST36) with EA, in comparison with control group by oligonucleotide chip microarray (Affymetrix GeneChip Rat Neurobiology U34 Array) and real-time RT-PCR. Sprague-Dawley (S-D) male rats were stimulated at the Zusanli (ST36) acupoint in restriction holder. Simultaneously the control group was given only holder stress without EA stimulation. In order to prove the appropriateness of EA treatment, we measured spleen NK cell activity with standard 51Cr release assay. NK cell activity of EA group was significantly increased comparing to control group. The microarray and PCR results show that EA treatment up-regulates expression of genes associated with 1) nerve growth such as NGF induced factor A and VGF, 2) signal transduction such as 5HT3 receptor subunit, AMPA receptor binding protein and Na-dependent neurotransmitter transporter, and 3) anti-oxidation such as superoxide dismutase and glutathione S-transferase. In addition, the activity of the anti-oxidative enzyme, SOD of hypothalamus, liver and RBC was enhanced compared to that of control. The list of differentially expressed genes may implicate further insight on the mechanism of acupuncture effects.
참고문헌 (46)
- "黃帝內經素問譯解", pp.219~ (1980)
- "Opioid reseptors", pp.69~70 (1995)
- "Inhibition of the analgesia inhibitory system by D-phenylalanine and proglumide", pp.385~91 (1991)
- "Role of endogenous interferon-gamma on the enhancement of splenic NK cell activity by electroacupuncture stimulation in mice", neuroimmu(l), pp.176~86 (1998)
- "Enhancement of splenic interferon-gamma and NK cytotoxicity by S36 acupoint acupuncture in F344 rats", pp.173~2 8 (1997)
- "site involved in pain modulation", pp.449~70 60 (1996)
- "The effect of medial hypothalamus lesions on pain control", pp.89~100 (1980)
- "pain management coupled to immune stimulation", pp.769~77 (1999)
- "Convenient assay for superoxide dismutase CRC handbook of free radicals and antioxidants in biomedicine", pp.287~8 (1988), CRC Press
- "potentiation of the effects of IGF-I", pp.679~87 (1993)
- "stimulates growth and IGF-I secretion in human intestinal smooth muscle by Ras-dependent activation of p38 MAP kinase and Erk1", 2 pathways, 2002, 20563~5 71
- "Regulation of the gonadotropin-releasing hormone receptor role of the GnRHR carboxyl-terminus", pp.149~56 (2002)
- "Oct-1 is involved in the transcriptional repression of the gonadotropin-releasing hormone receptor gene", endocri(logy 143), pp.4693~701 (2002)
- "Plant glutathione transferases", ge(me biol) (2002)
- "regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance", pp.445~600 (1995)
- "The isoenzymes of glutathione transferase", pp.357~417 (1985)
- "The synucleins. Genome", ge(me biol) (2002)
- "destruction as a programme", pp.96~102 (1996)
- "Cloning of a novel four repeat protein related to voltage-gated sodium and calcium channels", pp.2 231~3 6 (1999)
- "Cloning and characterization of human NTT5 and v7-3 two orphan transporters of the Na+", Cl- -dependent neurotransmitter transporter gene family, 2000, pp.ge(mics 70), 241~52
- "Characterization of a mammalian cDNA for an inactivating voltage-sensitive K+ channel", pp.471~7 83 (1991)
- "Critical role of axonal A-type K+ channels and axonal geometry in the gating of action potential propagation along CA3 pyramidal cell axons", pp.7436~51 (1998)
- "Role for neuronally derived fractalkine in mediating interactions between neurons and CX3CR1-expressing microglia", pp.10896~95 901 (1998)
- "Primary structure and functional expression of the 5HT3 receptor", pp.432~254 7 (1991)
- "Partial cDNA cloning and NGF regulation of a rat 5-HT3 receptor subunit", pp.121~4 (1993)
- "Nogo-A and Myelin-Associated Glycoprotein Mediate Neurite Growth Inhibition by Antagonistic Regulation of RhoA and Rac1", pp.10368~76 (2002)
- "modulates innate immune responses induced by endotoxin and Gram-negative bacteria", pp.456~60 (2001)
- "mechanisms of action and role in disease", pp.449~60 (2002)
- "Identification of macrophage migration inhibitory factor in rat spinal cord and its kinetics on experimental spinal cord injury", pp.409~30 (1997)
- "The effect of Moutan Cortex Radicis on gene expression profile of differentiated PC12 cells oxidative- stressed with hydrogen peroxide" (2002)
- "Protection of intracellular dopamine cytotoxicity by dopamine disposition and metabolism factors", pp.776~85 (2001)
- "Primary structure from amino acid and cDNA sequences of two Cu Zn superoxide dismutase variants from Xenopus laevis", pp.507~15 (1989)
- "Atomic structures of wild-type and thermostable mutant recombinant human Cu", pp.6109~89 13 (1992)
- "Primary structure of porcine Cu", pp.267~70 (1985)
- "Characterization of iron superoxide dismutase cDNAs from plants obtained by genetic complementation in Escherichia coli", pp.9903~87 7 (1990)
- "Characterization of the glutamate receptor-interacting proteins GRIP1 and GRIP2", pp.6930~41 (1999)
- "Regulation of the Egr-1 gene by tumor necrosis factor and interferons in primary human fibroblasts", pp.1345~9 (1992)
- "prototype of a zinc-finger family of transcription factors", pp.~1
- "processing and evolution of the neurohypophysial hormone-neurophysin precursors", pp.1197~70 207 (1988)
- "I., Nemeth, Z., Wiche, G", (iges), pp.2106~14 (2002), light chains determine distinct functional properties
- "A cortical neuropeptide with neuronal depressant and sleep-modulating properties", pp.242~381 5 (1996)
- "Identification of cortexin 82-residue membrane protein enriched in rodent cerebral cortex", pp.756~9 (1993)
- "a novel role for this neuronal and neuroendocrine polypeptide in the regulation of energy balance", neuroendocri(l)
- "The importance of free radicals and catalytic metal ions in human diseases", pp.89~193 (1985)
- "an early plant response to pathogen infection", pp.681~3 92 (1997)
- "Superoxide radical", pp.97~112 (1995)
