논문KCI 등재
알콜 탈수소효소의 안정도에 미치는 전기 자극의 효과
Effect of Electro-stimulation on Stability ofAlcohol Dehydrogenase from Horse Liver
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참고문헌
주제분류한의학
초록
Abstract
We investigated the activity and stability of alcohol dehydrogenase from horse liver (HLADH) under the electric stimulation. The activity and stability of alcohol dehydrogenase depended on electric output voltage, stimulation time, pulse duration and pulse interval, and temperature. HLADH retained about 23% of its activity in buffer but 78% in 10% trehalose solution under electric stimulation with 10V, 10min. The stabilizing of enzymes against electric stimulation by stabilizing additives showed a great potential use of enzymes in biotechnology and medical engineering fields.
참고문헌 (15)
- "Possibilities and problems of low frequency weak electromagnetic", pp.153~159 (1995)
- "Electrostimulation of macrophage NADPH oxidase by modulated high- frequency", pp.415~418 (1995)
- "Electric pulse-induced precipitation of biological macromolecules in electroporation", pp.249~254 (1999)
- "activity and properties of liver alcohol dehydrogenase in ionic water-in-oil microemulsion Eur l of Biochemistry", pp.609~15 (1987)
- "Probes of hydrogen tunneling with horse liver alcohol dehydrogenase at subzero", pp.2303~2311 (2001)
- "Structural basis for substrate specificity differences of horse liver alcohol", pp.12885~12897 (2000)
- "Activity and stability of alcohol oxidase from Hansenular sp", pp.171~176 (2004)
- "Stabilization of invertase by modification of sugar chains with", pp.347~351 (2000)
- "Effect of sucrose", raffinose mass ratios on the stability of co-lyophilized, 2001, 474~479
- "Stability of monoclonal IgM antibodies freezed in the", immu(logical methods), pp.1~7 (1995)
- "Effects of drying methods and additives on the structure and storage stability of subtilisin role of protein conformation and molecular mobility", pp.765~774 (2002)
- "Stabilization of proteases by entrapment in a new composite", pp.75~84 (1996)
- "Lipase immobilized on poly hydrogel for esterification" (1999)
- "Electromagnetic window effects on proliferation rate of Corynebacterium", pp.261~269 (1998)
- "Changes in cell proliferation due to environmental non-ionizing radiation 1", pp.109~114 (1995)
관련 논문AI추천
전기자극에 의한 알콜 산화효소의 활성도와 안정도연구김경숙, 박충웅, 이강민대한화학회 · 대한화학회지2004 · 제48권 제2호전기화학적 방법에 의한 효소의 pH 안정도곡선 도출권효식, 윤길중, 이범규대한화학회 · 대한화학회지2005 · 제49권 제3호전기화학적 방법에 의한 HRP의 최적 pH도출윤길중한국분석과학회 · 분석과학2003 · 제16권 제6호일산화탄소탈수소화효소를 이용한 이산화탄소의 전기화학적 환원에 미치는 pH와 온도의 영향김유성, 송지은, 신운섭, 신준원, 이상필, 이상희, 이호준, 임미란한국전기화학회 · 전기화학회지2007 · 제10권 제4호산화환원 효소의 최적 pH예측JihyeYang, JinRyounKim, YoungJeYoo대한화학회 · 대한화학회지2002 · 제46권 제5호체리 과산화효소 고정 과산화수소 정량 효소전극의 전기화학적 고찰윤길중, 이범규대한화학회 · 대한화학회지2015 · 제59권 제6호
