Modulation of Sarcodon Aspratus on Ion Currents-induced by Excitatory Neurotransmitters in Rat Periaqueductal Gray Neurons
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Sarcodon aspratus is the mushroom of Telephoracea which has been classified into Alphllophorales. The aqueous extract of Sarcodon aspratus is known to have anti-tumor activity, immune modulatory effect, and anti-oxidative action. The descending pain control system consists of three major components: the periaqueductal gray (PAG) of the midbrain, the rostroventral medulla including the nucleus raphe magnus, and the spinal dorsal horn. Glutamate is the primary excitatory neurotransmitter in the brain. Glutamate receptors are divided into two main families, namely metabotropic and ionotropic receptors. Glutamate ionotropic receptors are classified as N-methyl-D-aspartate (NMDA) receptor, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor, and kainate receptor. In the present study, the modulation of Sarcodon aspratus on the ion currents activated by glutamate, NMDA, AMPA, and kainate in the acutely dissociated PAG neurons was investigated by nystatin-perforated patch-clamp technique under voltage-clamp condition. Sarcodon aspratus increased glutamate- and NMDA-induced ion currents, while AMPA- and kainate-induced ion currents were not increased by Sarcodon aspratus. The present results show that Sarcodon aspratus may activate the descending pain control system in rat PAG neurons through NMDA receptor.
Abstract
Sarcodon aspratus is the mushroom of Telephoracea which has been classified into Alphllophorales. The aqueous extract of Sarcodon aspratus is known to have anti-tumor activity, immune modulatory effect, and anti-oxidative action. The descending pain control system consists of three major components: the periaqueductal gray (PAG) of the midbrain, the rostroventral medulla including the nucleus raphe magnus, and the spinal dorsal horn. Glutamate is the primary excitatory neurotransmitter in the brain. Glutamate receptors are divided into two main families, namely metabotropic and ionotropic receptors. Glutamate ionotropic receptors are classified as N-methyl-D-aspartate (NMDA) receptor, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptor, and kainate receptor. In the present study, the modulation of Sarcodon aspratus on the ion currents activated by glutamate, NMDA, AMPA, and kainate in the acutely dissociated PAG neurons was investigated by nystatin-perforated patch-clamp technique under voltage-clamp condition. Sarcodon aspratus increased glutamate- and NMDA-induced ion currents, while AMPA- and kainate-induced ion currents were not increased by Sarcodon aspratus. The present results show that Sarcodon aspratus may activate the descending pain control system in rat PAG neurons through NMDA receptor.
참고문헌 (23)
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