본문 바로가기
KERD
논문KCI 등재

산두근 추출물이 인체폐암세포의 COX-2 발현 및 PGE2 생성에 미치는 영향

Antiproliferative Effect of RST Associated with the Inhibition of Cyclooxygenase-2 Expression and Prostaglandin E2 Release in Human Lung Carcinoma Cells

지규용|엄현섭|김강태
한의병리학회 (The Society of Pathology in Korean Medicine)|2007.08|동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)|제21권 제4호|pp. 907-915

0

조회

0

다운로드

4

인용

0

참고문헌

주제분류한의학

초록

Abstract

In this study the effect of water extract of Sophora tonkinensis Gapnep (RST) was investigated on the growth of human lung carcinoma A549 cells. Exposure of A549 cells to RST resulted in the growth inhibition in a dose-dependent manner as measured by MTT assay. The antiproliferative effect by RST treatment in A549 cells was associated with morphological changes such as membrane shrinking and cell rounding up. RST treatment did not induce the cell cycle arrest and the levels of tumor suppressor p53 as well as cyclin-dependent kinase inhibitor p21(WAF1/CIP1). It was found that RST treatment decreased the levels of cyclooxygenase (COX) -2 mRNA and protein expression without significant changes in the expression of COX-1 and inducible nitric oxide synthase (iNOS) , which was correlated with a decrease in prostaglandin E2 (PGE2) synthesis. RST treatment also slightly inhibited the levels of human telomerase reverse transcriptase (hTERT) mRNA and protein expression, and the activity of telomerase. Taken together, these findings suggested that RST-induced inhibition of human lung carcinoma A549 cell growth was aoosciated with the inhibition of COX-2 expression and PGE2 production. These results provided important new insights into the possible molecular mechanisms of the anti-cancer activity of RST.

참고문헌 (37)

  1. "폐암의 조기진단", 15(2) (1998)
  2. "한국노인약물역학코호트에서 폐암 발생률, 사망률 및 생존율. 1994-1998", 24(2) (2002)
  3. "COX 저해제의 항암효과와 암 예방제로서 선택적 COX-2억제제", 2002(1) (2002)
  4. "인체 암 세포주에서 역전사 효소 기능 억제에 의한 Telomerase 활성도와 Telomerase Subunit 발현 변화" (2001)
  5. "정상 및 암세포에서 SiO₂, Crocidolite, MMF가 Telomerase 활성에 미치는 영향", 36(3) (1997)
  6. "정상인과 백혈병에서 telomerase 활성과 그 subunit인 human telomerase RNA (hTR) 및 human telomerase reverse transcriptase (hTRT) 와 p53 단백발현과의 관계에 대한 연구" (2001)
  7. "항 백혈병작용에 관련된 천연물의 자료조사", 17(3) (2003)
  8. "산두근이 PC12 세포 및 뇌해마 신경세포의 Glutamate Excitotoxicity에 미치는 영향" (2005)
  9. "산두근(Sophora subprostrata)의 화학성분 및 항암활성에 대한 연구" (2002)
  10. "Activities of fresh juice of Scutellaria barbata and warmed water extract of Radix Sophorae Tonkinensis on anti-proliferation and apoptosis of human cancer cell lines", 16(2), pp.337~341 (2005)
  11. "Wikyungtang inhibits proliferation of A549 human lung cancer cells via inducing apoptosis and suppressing cyclooxygenase-2 activity", 11, pp.853~856 (2004)
  12. "Telomeres, telomerase, and myc. An update", 462, pp.31~47 (2000)
  13. "Gerbil의 全腦虛血에 대한 廣豆根의 신경손상방어효능 연구" (2002)
  14. "廣豆根이 白鼠 中大腦動脈 閉鎖에 의한 局所腦虛血損傷에 미치는 保護效果" (2000)
  15. "두경부 편평세포암에서 Human Telomerase Reverse Transcriptase의 발현과 telomerase 활성도와의 상관관계" (2002)
  16. "Cyclin dependent kinase inhibitors", 29, pp.91~107 (1997)
  17. "Y. Cell cycle expression and p53 regulation of the cyclin-dependent kinase inhibitor p21. Oncogene 9", pp.2261~2268 (1994)
  18. "Regulation of the G2", M transition by p53, 2001, pp.20, 1803~1815
  19. "Functional analysis of the transforming growth factor β responsive elements in the WAF1", Cip1, pp.1995, 270[DOI]
  20. "Regulation of p21WAF1", CIP1 expression by p53-independent pathways, 1996, pp.12, 1557~1564
  21. "p53-independent induction of p21 (WAF1", CIP1) reduction of cyclin B1 and G2, pp.2000, 91[DOI]
  22. "D. p21 is a universal inhibitor of cyclin kinases. Nature", pp.701~704 (1993)
  23. "Novel form of p21 (WAF1", CIP1, M arrest, 1996[DOI]
  24. "Nuclear accumulation of p21Cip1 at the onset of mitosis: a role at the G2", M-phase transition, 1998, pp.18, 546~557
  25. "Role of the CDK Inhibitor p27 (Kip1) in mammary development and carcinogenesis: Insights from knockout mice", 9, pp.55~66 (2004)
  26. "COX-2 inhibition and prevention of cancer", 15, pp.821~833 (2001)
  27. "Nonsteroidal anti-inflammatory drugs as anticancer agents: mechanistic, pharmacologic, and clinical issues", 94, pp.252~266 (2002)
  28. "Is COX-2 inhibition a panacea for cancer prevention?", 94, pp.613~614 (2001)
  29. "Cyclooxygenase-2: a novel target for cancer chemotherapy?", 127, pp.411~417 (2001)
  30. "Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-κB activation", pp.243~481 (2001)
  31. "Cyclooxygenase inhibitors suppress angiogenesis and reduce tumor growth in vivo", 79, pp.1469~1477 (1999)
  32. "Therapeutic potential of inhibition of the NF-κB pathway in the treatment of inflammation and cancer", 107, pp.135~142 (2001)
  33. "Activity, function, and gene regulation of the catalytic subunit of telomerase (hTERT)", 269, pp.1~12 (2001)
  34. "ATM-dependent telomere loss in aging human diploid fibroblasts and DNA damage lead to the post-translational activation of p53 protein involving poly(ADP-ribose) polymerase", 16, pp.6018~6033 (1997)
  35. "Complex regulatory mechanisms of telomerase activity in normal and cancer cells: How can we apply them for cancer therapy", 21, pp.688~697 (2002)
  36. "DNA damage-induced cell cycle checkpoints involve both p53-dependent and -independent pathways: role of telomere repeat binding factor 2", 85, pp.898~901 (2001)
  37. "Telomeres, telomerase, and myc. An update", 462, pp.31~47 (2000)

관련 논문AI추천

인체폐암세포의 증식 및 prostaglandin E2 생성에 미치는 청조구폐탕의 영향에 관한 연구김훈, 감철우, 박동일, 변미권, 임재형한의병리학회 · 동의생리병리학회지2006 · 제20권 제4호인체유방암세포에서 Cyclooxygenase-2 활성 및 Prostaglandin E2 생성에 미치는 어성초 추출물의 영향박철, 박동일, 정일홍, 조인주, 최영현한의병리학회 · 동의생리병리학회지2006 · 제20권 제3호인체폐암세포에서의 prostaglandin E2 생성과 Telomere 활성에 미치는 청조구폐탕의 영향에 관한 연구김훈, 박동일대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2006 · 제19권 제2호사백산 추출물에 의한 인체 폐암세포의 Apoptosis 유도 기전에 관한 연구감철우, 강병령, 박동일, 이재훈, 최영현한의병리학회 · 동의생리병리학회지2003 · 제17권 제2호길경 수용액 추출물에 의한 인체 폐암세포의 성장억제 기전 연구감철우, 강락원, 박동일, 이재훈, 최병태, 최영현한의병리학회 · 동의생리병리학회지2003 · 제17권 제1호千金葦莖湯의 인체 폐암세포 증식억제에 관한 연구박동일, 박봉규한의병리학회 · 동의생리병리학회지2004 · 제18권 제4호

이 간행물의 다른 논문 (17건)

引火歸原의 병기론 연구김영목, 조원준pp. 795-802燥證에 대한 연구문성식, 강정수pp. 815-821夢診의 역사에 관한 연구강정수, 강동윤pp. 842-848加味復元通氣散의 항염작용에 대한 실험적 연구김수민, 신선미, 이정은, 김지혜, 유동열, 임현정pp. 860-868石菖蒲의 면역조절 및 항암효과이창현, 김남석, 이규희, 김태호, 신태용, 김순근, 전훈, 정승일, 임종필, 권진, 오찬호, 배진범pp. 869-873
원문 보기