蘇木 물추출물의 G2/M기 정지를 통한 U937세포의 성장억제 효과
Caesalpinia sappan L. Induces G2/M Phase Cell Cycle Arrest in Human Lymphoma U937 Cells
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초록
Caesalpinia sappan L. (C. sappan) has long been used in traditional medicine as an emmenagogue, hemostatic and anti-inflammatory agent. The present study investigated the effects of water extract of C. sappan in human lymphoma U937 cells. The proliferation of U937 cells was decreased by C. sappan in a dose-dependently manner. Anti-proliferative effect of C. sappan on U937 cells was associated with G2/M phase arrest, which was mediated by regulating the expression of p21 protein. Moreover, phosphorylation of JNK and p38 was increased by C. sappan. Blockade of JNK and p38 was significantly inhibited C. sappan-induced G2/M phase arrest. Taken together, these results suggest that Anti-proliferative effect of C. sappan on U937 is assocated with G2/M phase cell cycle arrest by expression of p21 protein and, JNK and p38 activation.
Abstract
Caesalpinia sappan L. (C. sappan) has long been used in traditional medicine as an emmenagogue, hemostatic and anti-inflammatory agent. The present study investigated the effects of water extract of C. sappan in human lymphoma U937 cells. The proliferation of U937 cells was decreased by C. sappan in a dose-dependently manner. Anti-proliferative effect of C. sappan on U937 cells was associated with G2/M phase arrest, which was mediated by regulating the expression of p21 protein. Moreover, phosphorylation of JNK and p38 was increased by C. sappan. Blockade of JNK and p38 was significantly inhibited C. sappan-induced G2/M phase arrest. Taken together, these results suggest that Anti-proliferative effect of C. sappan on U937 is assocated with G2/M phase cell cycle arrest by expression of p21 protein and, JNK and p38 activation.
참고문헌 (38)
- 장진섭, "도해", pp.666~667 (1990), 생약대사전
- 김창민, "중약대사전", pp.2420~2423 (1997), 청담출판사
- Park, K.J, "Cytotoxic effect of Korean traditional prescription on the human gastric cancer cell lines", Kor. J. Pharmacogn, 28, pp.233~238 (1997)
- Lim, D.K, "Antioxidative activity of some solvent extract from Caesalpinia sappan L", 한국식품과학회, 28, pp.77~82 (1996)
- Kim, Y.S, "Inhibitory effects of herbal medicines on hyaluronidase activity", Kor. J. Pharmacogn, 26, pp.265~272 (1995)
- Jeon, W.K, "In vitro studies on the anticancer effect and topoisomerase I inhibition activity of Caesalpinia sappan L. extract", Kor. J. pharmacogn, 30, pp.1~6 (1999)
- Moon, C.K, "Effects of Brazilin on erythrocyte deformability and its regulated biochemical factors in streptozotocin induced diabetic rats, Arch", Pharm, Res, 11, pp.149~154 (1988)
- Moon, C.K, "Brazilin protects cultured rat hepatocytes from BrCCl3- induced toxicity", Drug Chem. Toxicol, 15, pp.81~91 (1992)
- Hwang, G.S, "Effects of Brazilin on the phopholipase A2 activity and changes of intracellular free calcium concentration in rat platelets", Arch. Pharm. Res, 21, pp.774~778 (1998)
- Kim, S.G, "Brazilin inhibits activities of protein kinase C and insulin receptor serine kinase in rat liver", Arch. Pharm. Res, 21, pp.140~146 (1998)
- 이강대, "brazilin as a new sunless tanning agent", 대한화장품학회지, 23, pp.81~85 (1997)
- 강병수, "본초학", pp.299~ (2000), 영림사
- 이상인, "한약임상응용", pp.438~ (1998), 전통의학연구원
- Koeffler, H.P, "Induction of differentiation of human acute myelogenous leukemia cells: Therapeutic implication", Blood, 62, pp.709~721 (1983)
- Kim, J.I, "Induction of differentiation on the human histocytic lymphoma cell line U-937 by Costunolide", Kor. J. Pharmacogn, 30, pp.7~11 (1999)
- Covacci, V, "Magnesium restriction induces granulocytic differentiation and expression of p27Kip1 in human leukemic HL-60 cells", J. Cell Biochem, 70, pp.313~322 (1998)
- Sherr, C.J, "Inhibitors of mammalian G1 cyclin-dependent kinases", Genes Dev, 9, pp.1149~1163 (1995)
- Brugarolas, J, "Radiation-induced cell cycle arrest compromised by p21 deficiency", Nature, 377, pp.552~557 (1995)
- Furukawa, Y, "Transforming growth factor-beta inhibits phosphorylation of the retinoblastoma susceptibility gene product in human monocytic leukemia cell line JOSK-I", J. Biol. Chem, 267, pp.17121~17127 (1992)
- Hui, E.K, "Cell cycle phase-dependent effect of retinoic acid on the induction of granulocytic differentiation in HL-60 promyelocytic leukemia cells. Evidence for sphinganine potentiation of retinoic acid-induced differentiation", FEBS Lett, 318, pp.193~199 (1993)
- Cooper, S, "Revisiting the relationship of the mammalian G1 phase to cell differentiation", J. Theor. Biol, 208, pp.399~402 (2001)
- Wang, Q.M, "Cyclin-dependent kinase inhibitor p27 as a mediator of the G1-S phase block induced by 1,25-dihydroxyvitamin D3 in HL60 cells", Cancer Res, 56, pp.264~267 (1996)
- Futamura, M, "Trichostatin A inhibits both ras-induced neurite outgrowth of PC12 cells and morphological transformation of NIH3T3 cells", Oncogene, 10, pp.1119~1123 (1995)
- Parker, S.B, "p53-independent expression of p21Cip1 in muscle and other terminally differentiating cells", Science, 267, pp.1024~1027 (1995)
- Zhang, W, "p53-independent induction of WAF1", CIP1 in human leukemia cells is correlated with growth arrest accompanying monocyte, Cancer Res, pp.1995, 55[DOI]
- Wada, T, "Mitogen-activated protein kinases in apoptosis regulation", Oncogene, 23, pp.2838~2849 (2004)
- Guyton, K.Z, "Activation of mitogen-activated protein kinase by H2O2. Role in cell survival following oxidant injury", J. Biol. Chem, 271, pp.4138~4142 (1996)
- Yoshizumi, M, "Signal transduction of reactive oxygen species and mitogen-activated protein kinases in cardiovascular disease", J. Med. Invest, 48, pp.11~24 (2001)
- Ramachandiran, S, "Mitogen-activated protein kinases contribute to reactive oxygen species-induced cell death in renal proximal tubule epithelial cells", Chem. Res. Toxicol, 15, pp.1635~1642 (2002)
- Dabrowski, A, "Reactive oxygen species activate mitogen-activated protein kinases in pancreatic acinar cells", Pancreas, 21, pp.376~384 (2000)
- Kannan, K, "Oxidative stress and apoptosis", Pathophysiology, 7, pp.153~163 (2000)
- Feuerstein, G.Z, "Apoptosis in cardiac diseases: stress- and mitogen-activated signaling pathways", Cardiovasc. Res, 45, pp.560~569 (2000)
- Guadagno, T.M, "Requirement for MAPK activation for normal mitotic progression in Xenopus egg extracts", Science, 282, pp.1312~1315 (1998)
- Wright, J.H, "Mitogen-activated protein kinase kinase activity is required for the G(2)", M transition of the cell cycle in mammalian fibroblasts, 1996, pp.96, 11335~11340
- Bulavin, D.V, "Initiation of a G2", M checkpoint after ultraviolet radiation requires p38 kinase, 2001, pp.411, 102~107
- Wang, X, "Involvement of the MKK6-p38gamma cascade in gamma-radiation-induced cell cycle arrest", Mol. Cell. Biol, 20, pp.4543~4552 (2000)
- Lee, S.J, "Cordycepin causes p21WAF1-mediated G2", M cell-cycle arrest by regulating c-Jun N-terminal kinase activation in human bladder cancer cells, 2009, pp.189, 145~151
- Zhu, N, "Gadd45-alpha and Gadd45-gamma utilize p38 and JNK signaling pathways to induce cell cycle G2", M arrest in Hep-G2 hepatoma cells, 2009, pp.36, 2075~2085
