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淸營金飮이 자유산소라디칼과 염증매개인자에 미치는 영향

Anti-inflammatory Effects of New Herbal Formula Cheongyeoungkeumyeum

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초록

The purpose of this study is to verify the anti-inflammatory effectiveness of Cheongyeoungkeumyeum(CYK) including seven herbal medicines of that major effectiveness is to clear heat, to relieve fire toxicity and to clear damp-heat. To evaluate anti-inflammatory effectiveness of CYK, we measured the production of reactive oxygen species(ROS), nitric oxide(NO) cyclooxygenase-2(COX-2) and in TNF-α LPS-activated Raw 264.7 cells. Cell viability was determined by MTT assay. The concentrations of ROS and relative level of NO were measured with DPPH assay and Griess reagent, respectively. COX-2 and TNF-α were detected by enzyme immuno assay(EIA) and enzyme-linked immunosorbent assay(ELISA). As a result, we found that CYK suppressed LPS-induced ROS and NO production in a dose-dependent manner. Also CYK significantly inhibited LPS-induced COX-2 activity and the release of TNF-α. These results indicate that the CYK may have an anti-inflammatory agent for the treatment of various inflammatory disease.

Abstract

The purpose of this study is to verify the anti-inflammatory effectiveness of Cheongyeoungkeumyeum(CYK) including seven herbal medicines of that major effectiveness is to clear heat, to relieve fire toxicity and to clear damp-heat. To evaluate anti-inflammatory effectiveness of CYK, we measured the production of reactive oxygen species(ROS), nitric oxide(NO) cyclooxygenase-2(COX-2) and in TNF-α LPS-activated Raw 264.7 cells. Cell viability was determined by MTT assay. The concentrations of ROS and relative level of NO were measured with DPPH assay and Griess reagent, respectively. COX-2 and TNF-α were detected by enzyme immuno assay(EIA) and enzyme-linked immunosorbent assay(ELISA). As a result, we found that CYK suppressed LPS-induced ROS and NO production in a dose-dependent manner. Also CYK significantly inhibited LPS-induced COX-2 activity and the release of TNF-α. These results indicate that the CYK may have an anti-inflammatory agent for the treatment of various inflammatory disease.

참고문헌 (34)

  1. Wolfe, M. M, "Gastroinstestinal toxicity of nonsteroidal antiflammatory drugs", N Engl J Med, 340, pp.1888~1899 (1999)
  2. O'Leary, K. A, "Effect of flavonoids and Vitamin E on cyclooxygenase-2 (COX-2) transcription", Mutat Res, 551, pp.245~254 (2004)
  3. Kujubu, D. A, "TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase", cyclooxygenase homologue, 1991, pp.266, 12866~12872
  4. Capone, ml, "Pharmacodynamic of cyclooxygenase inhibitors in humans", Prostaglandins Other Lipid Mediat, 82, pp.85~94 (2007)
  5. Barland, C. O, "Imiquimod induced interleukin-1 alpha stimualtion improves barrier homeostasis in aged murine epidermis", J Invest Dermatol, 122, pp.330~336 (2004)
  6. Heinzmann, A, "Mouse models for the genetic dissection of atopy", Int Arch Allergy Immunol, 127, pp.170~180 (2002)
  7. Surh, Y. J, "Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation", Mutat Res, 480-481, pp.243~481 (2001)
  8. Bowie, A, "Oxidative stress and nuclear factor-kappaB activation: a reassessment of the evidence in the light of recent discoveries", Biochem Pharmacol, 59(1), pp.13~23 (2000)
  9. Seo, J. Y, "Effect of a fermented ginsing extract, BST 204, on the expression of cyclooxygenase-2 in murine macrophages", Int. Immunopharmacol, 5(5), pp.929~936 (2005)
  10. Blos, M. S, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~ (1958)
  11. Knipping, K, "In vitro and in vivo anti-allergic effects of Arctium lappa L", Exp Biol Med, 233(11), pp.1469~1477 (2008)
  12. Sohn, E. H, "Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L", Clin Mol Allergy, 9(1), pp.4~ (2011)
  13. Kim, J. H, "Fraxinellone inhibits lipopolysaccharide-induced inducible nitric oxide synthase and cyclooxygenase-2 expression by negatively regulating nuclear factor-kappa B in RAW 264.7 macrophages cells", Biol Pharm Bull, 32(6), pp.1062~1068 (2009)
  14. Saeedi, M, "The treatment of atopic dermatitis with licorice gel", J Dermatolog Treat, 14(3), pp.153~157 (2003)
  15. Kim, J. Y, "Isoliquiritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-kappaB in RAW 264.7 macrophages", Eur J Pharmacol, 584(1), pp.175~184 (2008)
  16. Chan, B. C, "Traditional Chinese medicine for atopic eczema: PentaHerbs formula suppresses inflammatory mediators release from mast cells", J Ethnopharmacol, 120(1), pp.85~91 (2008)
  17. Shang, X, "Lonicera japonica Thunb.: ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine", J Ethnopharmacol, 138(1), pp.1~21 (2011)
  18. Kwon, S. H, "Lonicera japonica THUNB. protects 6-hydroxydopamine-induced neurotoxicity by inhibiting activation of MAPKs, PI3K", Akt, and NF-κB in SH-SY5Y cells, 2012, pp.50(3-4), 797~807
  19. Hak Ju Lee, "Protection of Prenylated Flavonoids from Mori Cortex Radicis (Moraceae) against Nitric Oxide-induced Cell Death in Neuroblastoma SH-SY5Y Cells", Archives of Pharmacal Research, 35(1), pp.163~170 (2012), 대한약학회
  20. Kim, H. J, "Cortex Mori Radicis extract exerts antiasthmatic effects via enhancement of CD4(+)CD25(+)Foxp3(+) regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model", J Ethnopharmacol, 138(1), pp.40~46 (2011)
  21. Kang, H. S, "Anti-inflammatory activity of arctigenin from Forsythiae Fructus", J Ethnopharmacol, 116(2), pp.305~312 (2008)
  22. Hu, C, "Evaluation of antioxidant and prooxidant activities of bamboo Phyllostachys nigra var. Henonis leaf extract in vitro", Agric Food Chem, 48(8), pp.3170~3176 (2000)
  23. Kim, S. H, "Inhibition of interleukin-12 production in mouse macrophages via suppression of nuclear factor-kappa B binding activity by Phyllostachys nigro var. henonis", IMMUNOPHARMACOL IMMUNOTOXICOL, 29(1), pp.131~139, TAYLOR & FRANCIS INC
  24. Edman, U, "Lifespan extension by dietary restriction is not linked to protection against somatic DNA damage in Drosophila melanogaster", Aging Cell, 8(3), pp.331~338 (2009)
  25. Lawless, M. W, "Oxidative stress induced lung cancer and COPD: opportunities for epigenetic therapy", J Cell Mol Med, 13(9A), pp.2800~2821 (2009)
  26. Csiszar, A, "Anti-oxidative and anti-inflammatory vasoprotective effects of caloric restriction in aging: role of circulating factors and SIRT1", Mech Ageing Dev, 130(8), pp.518~527 (2009)
  27. Rao, K. S, "Free radical induced oxidative damage to DNA: relation to brain aging and neurological disorders", Indian J Biochem Biophys, 46(1), pp.9~15 (2009)
  28. Kim, G. D, "α-Lipoic acid suppresses the development of DNFB-induced atopic dermatitis-like symptoms in NC", Nga mice, 2011, pp.20(2), 97~101
  29. Kyung Sun Lee, So Ri Kim, "A novel thiol compound, N-acetylcysteine amide, attenuatesallergic airway disease by regulating activation of NF-κB andhypoxia-inducible factor-1α", Experimental and Molecular Medicine, 39(6), pp.756~768 (2007), 생화학분자생물학회
  30. Nathan, C, "Nitric oxide as a secretory product of mammalian cells", FASEB J, 6(12), pp.3051~3064 (1992)
  31. Weisz, A, "Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-gamma, bacterial lipopolysaccharide and NG-monomethyl-L-arginine", Biochem J, 316, pp.209~215 (1996)
  32. Fredenburgh, L. E, "Cyclooxygenase-2 inhibition and hypoxia-induced pulmonary hypertension: effects on pulmonary vascular remodeling and contractility", Trends Cardiovasc Med, 19(2), pp.31~37 (2009)
  33. Steinhoff, M, "Proteinase-activated receptor-2 mediates itch: a novel pathway for pruritus in human skin", J Neurosci, 23(15), pp.6176~6180 (2003)
  34. Elias, P. M, "Abnormal skin barrier in the etiopathogenesis of atopic dermatitis", Curr Allergy Asthma Rep, 9(4), pp.265~272 (2009)

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