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防己黃芪湯이 생쥐의 비만관련 장내분비세포와 신경펩타이드에 미치는 영향

Effects of Banggihwanggi-Tang on Obesity-related Enteroendocrine Cells and Neuropeptides in Mice

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초록

To determine the effects of Banggihwanggi-tang(BGHGT) on obesity, the obesity-related factors[gastrin, calcitonin gene related peptide(CGRP), serotonin, ghrelin, obestatin, glucagon-like peptide-1(GLP-1), insulin, orexin, leptin] were investigated in the stomach, pancreas, brain of mice by immunohistochemical(IHC) methods for 4 weeks. The change of body weight was more reduced in BGHGT administered group than that of control group. The IHC density of the gastrin and CGRP positive cells on pylorus was higher in BGHGT administered group than that of control group. The number of ghrelin immunoreactive cells on stomach was lower in BGHGT administered group than that of control group. The IHC of GLP-1 positive cells did not observe in the stomach of BGHGT administered groups. The IHC density of GLP-1 in the pancreas was lower in BGHGT administered group than that of control group. The IHC density of insulin positive cells in the pancreas was lower in BGHGT administered group than that of control group. The IHC density of orexin positive neurons in the diencephalon was slightly higher in BGHGT administered group than that of control group. The IHC density of NPY and leptin positive neurons was slightly higher in BGHGT administered group than that of control group. The IHC density of serotonin positive neurons was higher in BGHGT administered group than that of control group. Therefore, we conclude that BGHGT activates appetite inhibitor through appetite related enteroendocrine cells and neuropeptides in stomach, pancreas and brain, and this activation may also be responsible for the inhibition of feeding behavior.

Abstract

To determine the effects of Banggihwanggi-tang(BGHGT) on obesity, the obesity-related factors[gastrin, calcitonin gene related peptide(CGRP), serotonin, ghrelin, obestatin, glucagon-like peptide-1(GLP-1), insulin, orexin, leptin] were investigated in the stomach, pancreas, brain of mice by immunohistochemical(IHC) methods for 4 weeks. The change of body weight was more reduced in BGHGT administered group than that of control group. The IHC density of the gastrin and CGRP positive cells on pylorus was higher in BGHGT administered group than that of control group. The number of ghrelin immunoreactive cells on stomach was lower in BGHGT administered group than that of control group. The IHC of GLP-1 positive cells did not observe in the stomach of BGHGT administered groups. The IHC density of GLP-1 in the pancreas was lower in BGHGT administered group than that of control group. The IHC density of insulin positive cells in the pancreas was lower in BGHGT administered group than that of control group. The IHC density of orexin positive neurons in the diencephalon was slightly higher in BGHGT administered group than that of control group. The IHC density of NPY and leptin positive neurons was slightly higher in BGHGT administered group than that of control group. The IHC density of serotonin positive neurons was higher in BGHGT administered group than that of control group. Therefore, we conclude that BGHGT activates appetite inhibitor through appetite related enteroendocrine cells and neuropeptides in stomach, pancreas and brain, and this activation may also be responsible for the inhibition of feeding behavior.

참고문헌 (70)

  1. Field, A. E., "Impact of overweight on the risk of developing common chronic diseases during a 10-year period", Arch Intern Med, 161, pp.1581~1586 (2001)
  2. Must, A., "The disease burden associated with overweight and obesity", JAMA, 282, pp.1523~1529 (1999)
  3. Polesel, J., "The impact of obesity and diabetes mellitus on the risk of hepatocellular carcinoma", Ann Oncol, 20, pp.353~357 (2009)
  4. Hu, G., "Body mass index and the risk of Parkinson disease", Neurology, 67, pp.1955~1959 (2006)
  5. Whitmer, R. A., "Body mass index in midlife and risk of Alzheimer disease and vascular dementia", Curr Alzheimer Res, 4, pp.103~109 (2007)
  6. Druce, M., "The regulation of appetite", Arch Dis Child, 91, pp.183~187 (2006)
  7. Yanovski, S. Z., "Obesity", N Engl J Med, 346, pp.591~602 (2002)
  8. 김철희, "비만의 약물치료", 임상당뇨병, 9, pp.173~177 (2008)
  9. 이재성, "한방치료의 체지방 및 복부비만 감소효과", 대한한방비만학회지, 1, pp.33~42 (2001)
  10. 홍원식, "정교황제내경", pp.61~ (1974), 동양의학연구원 출판부
  11. 趙金譯, "중의증상감별진단학", pp.43~ (1987), 인민위생출판사
  12. 虞天民, "醫學正傳", pp.75~ (1986), 성보사
  13. 李梃, "醫學入門", pp.108~323 (1974), 대성문화사
  14. 張仲景, "金匱要略方論", pp.21~70 (1985), 성보사
  15. 中醫硏究員, "中醫症狀鑑別診斷學", pp.43~ (1987), 인민위생출판사
  16. 유은주, "고지방 식이로 유도된 비만 생쥐에서 창출약침의 항비만 효과", 대한침구의학회지, 27(6), pp.31~42 (2010), 대한침구의학회
  17. 김진혁, "마황천오약침이 비만에 미치는 영향", 대한침구의학회지, 26(5), pp.77~83 (2009), 대한침구의학회
  18. 정지윤, "전침이 복부비만 성인의 비만관련 지표에 미치는 영향 -무작위배정 단일 맹검 예비연구-", 대한침구의학회지, 27(6), pp.43~57 (2010), 대한침구의학회
  19. 윤태숙, "Anti-obesity Activity of Extract from Saussurea lappa", 한국약용작물학회지, 18(3), pp.151~156 (2010), 한국약용작물학회
  20. 이영종, "防己黃?湯 및 防己茯?湯이 高脂血症 흰쥐에 미치는 效果", 대한본초학회지, 20(2), pp.149~157 (2005), 대한본초학회
  21. 양동혁, "太陰調胃湯과 太陰調胃培麻黃湯이 비만 흰쥐에 미치는 영향", 대한본초학회지, 25(3), pp.103~109 (2010), 대한본초학회
  22. 謝鳴, "中醫方劑現代硏究", pp.44~1442 (1997), 學苑出版社
  23. 김수익, "防己黃芪湯 전탕액이 백서의 실험적 비만증과 전지방세포인 3T3-L1의 지방세포 분화에 미치는 영향", 한방재활의학과학회지, 7, pp.120~135 (1997)
  24. 이응세, "防己黃芪湯이 비만유도 흰쥐의 간 및 부고환 지방조직과 혈청지질의 변화에 미치는 영향", 한방재활의학과학회지, 5, pp.1~37 (1995)
  25. 송용선, "防己黃芪湯 및 구기자가 비만백서의 체중에 미치는 영향", 한방재활의학과학회지, 1, pp.25~43 (1991)
  26. 노의준, "古方類聚", pp.306~833 (2009), 도서출판 고방
  27. 정창현, "藥徵", pp.129~135 (2006), 청홍(지상사)
  28. Hsu, S. M., "Use of avidin-biotin-peroxidase complex(ABC)in immunoperoxidase techniques : A comparison between ABC and unlabeled antibody(PAP)procedures", J Histochem Cytochem, 29, pp.577~580 (1981)
  29. 대한비만학회, "임상비만학", pp.184~189 (1995), 고려의학
  30. Hubert, H. B., "Obesity as an independent risk factor for cardiovascular disease: A-26 year follow up of participants in the Framingham heart study", Circulation, 67, pp.968~977 (1982)
  31. Isselbacher, K. J., "Harrison, s principles of internal medicine", pp.446~452 (1994), McGraw-Hill
  32. Li, G., "Obesity, coronary heart disease risk factors and diabetes in chinese : an approach to the criteria of obesity in the chinese population", Obes Rev, 3, pp.167~172 (2002)
  33. 전국한의과대학본초학교수, "본초학", pp.263~264 (1991), 영림사
  34. 조호근, "防己의 투여가 비만 유발 쥐의 생리기능과 DNA Chip을 통한 유전자 발현에 미치는 영향에 대한 연구", 대한한방부인과학회지, 20(4), pp.41~55 (2007), 대한한방부인과학회
  35. 송용선, "防己黃芪湯 및 구기자가 비만백서의 체중에 미치는 영향", 원광한의학, 2, pp.102~120 (1992)
  36. 전국한의과대학본초학교수, "본초학", pp.534~536 (1991), 영림사
  37. 김학준, "황기가 흰쥐의 식이성 고지혈증에 미치는 영향", 동의생리병리학회지, 22(3), pp.575~579 (2008), 대한동의병리학회
  38. 전국한의과대학본초학교수, "본초학", pp.289~290 (1991), 영림사
  39. Satoh, K., "The effects of crude drugs using diuretic on horse kidney(Na++K+)-adenosine triposphatase", Yakugaku Zasshi, 111, pp.138~145 (1991)
  40. 고석태, "蒼朮이 개의 신기능에 미치는 영향", 대한약제학회지, 3, pp.23~33 (1973)
  41. Näslund, E., "Appetite signaling : From gut peptides and enteric nerves to brain", Physiology & Behavior, 92, pp.256~262 (2007)
  42. Lavine, J. A., "Gastrointestinal hormones and the regulation of β-cell mass", Ann NY Acad Sci, 1212, pp.41~58 (2010)
  43. Dockray, G., "Gastrin : old hormone, new functions", Pflugers Arch, 449, pp.344~355 (2005)
  44. Rehfeld, J. F., "Incretin physiology beyond glucagon-like peptide 1 and glucose-dependent insulinotropic polypeptide: cholecystokinin and gastrin peptides", Acta Physiol, 201, pp.405~411 (2011)
  45. Evangelista, S, "Role of calcitonin gene-related Peptide in gastric mucosal defence and healing", Curr Pharm Des, 15, pp.3571~3576 (2009)
  46. Zelissen, P. M., "Calcitonin gene-related peptide in human obesity", Peptides, 12, pp.861~863 (1991)
  47. Kojima, M., "Ghrelin is a growth-hormone-releasing acylated peptide from stomach", Nature, 402, pp.656~660 (1999)
  48. Guan, X. M., "Distribution of mRNA encoding the growth hormone secretagogue receptor in brain and peripheral tissues", Brain Res Mol Brain Res, 48, pp.23~29 (1997)
  49. Katayama, M., "Developmentally and regionally regulated expression of growth hormone secretagogue receptor mRNA in rat brain and pituitary gland", Neuroendocrinology, 72, pp.333~340 (2000)
  50. Wren, A. M., "Ghrelin enhances appetite and increases food intake in humans", J Clin Endocrinol Metab, 86, pp.5992~ (2001)
  51. Nakazato, M., "A role for ghrelin in the central regulation of feeding", Nature, 409, pp.194~198 (2001)
  52. Hewson, A. K., "The rat arcuate nucleus integrates peripheral signals provided by leptin, insulin, and a ghrelin mimetic", Diabetes, 51, pp.3412~3419 (2002)
  53. Holst, J. J, "Enteroglucagon", Annu Rev Physiol, 59, pp.257~271 (1997)
  54. Kreymann, B., "Glucagon-like peptide-1 7-36 : a physiological incretin in man", Lancet, 2, pp.1300~1304 (1987)
  55. Schwartz, M. W., "Central nervous system control of food intake", Nature, 404, pp.661~671 (2000)
  56. Kalra, S. P., "Interacting appetite-regulating pathways in the hypothalamic regulation of body weight", Endocr Rev, 20, pp.68~100 (1999)
  57. Cone, R. D, "The arcuate nucleus as a conduit for diverse signals relevant to energy homeostasis", Int J Obes Relat Metab Disord, 25(Suppl. 5), pp.S63~S67 (2001)
  58. Simpson, K. A., "Hypothalamic regulation of food intake and clinical therapeutic applications", Arq Bras Endocrinol Metab, 53, pp.120~128 (2009)
  59. Nambu, T., "Distribution of orexin neurons in the adult rat brain", Brain Res, 827, pp.243~260 (1999)
  60. Saper, C. B, "Staying awake for dinner : hypothalamic integration of sleep, feeding, and circadian rhythms", Prog Brain Res, 153, pp.243~252 (2006)
  61. Cowley, M.A, "Leptin activates anorexigenic POMC neurons through a neural network in the arcuate nucleus", Nature, 411, pp.480~484 (2001)
  62. Wang, L., "Fos expression in the brain induced by peripheral injection of CCK or leptin plus CCK in fasted lean mice", Brain Res, 791, pp.157~166 (1998)
  63. Zhang, Y., "Positional cloning of the mouse obese gene and its human homologue", Nature, 372, pp.425~432 (1994)
  64. Campfield, L. A., "The OB protein(leptin)pathway-link between adipose tissue mass and central neural networks", Horm Metab Res, 28, pp.619~632 (1996)
  65. Hamao, M., "Anti-obesity effects of the methanolic extract and chakasaponins from the flower buds of Camellia sinensis in mice", Bioorg Med Chem, 19, pp.6033~6041 (2011)
  66. Dube, M. G., "Evidence that neuropeptide Y is a physiological signal for normal food intake", Brain Res, 646, pp.341~344 (1994)
  67. Bai, F. L., "An arcuato-paraventricular and-dorsomedial hypothalamic neuropeptide Y-containing system which lacks noradrenaline in the rat", Brain Res, 331, pp.172~175 (1985)
  68. Stanley, B. G., "Neuropeptide Y injected in the paraventricular hypothalamus: a powerful stimulant of feeding behavior", Proc Natl Acad Sci USA, 82, pp.3940~3943 (1985)
  69. Chronwall, B. M, "Anatomy and physiology of the neuroendocrine arcuate nucleus", Peptides, 6(Suppl 2), pp.1~11 (1985)
  70. Smith, AI., "Proopiomelanocortin processing in the pituitary, central nervous system, and peripheral tissues", Endocr Rev, 9, pp.159~179 (1988)

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