Cisplatin에 의한 耳毒性에서 苓桂朮甘湯의 보호 효과
Protective Effects of Younggyechulgam-tang in Cisplatin-induced Ototoxicity
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초록
The water extract of Younggyechulgam-tang (YGCGT) has been traditionally used in treatment of tinnitus in Oriental Medicine. However, little is known about the mechanism by which YGCGT rescues ototoxicity. The purpose of the present study is to investigate the protective effect of YGCGT against cisplatin-induced toxicity in HEI-OC1 auditory cells, organotypic cultures of cochlear explants from three-day postnatal rats (P3). Pretreatment with YGCGT ameliorated apoptotic death induced by cisplatin in HEI-OC1 cells and organotypic cultures of Corti’s organ. YGCGT pretreatment also significantly suppressed cisplatin-induced increases in intracellular reactive oxygen species (ROS). Treatment with YGCGT resulted in an increased expression of HO-1 and Bcl-2. These results suggest that YGCGT induced HO-1 signaling pathway, which ultimately prevents free radical stresses from cisplatin and further contributes to protect auditory sensory hair cells from free radicals produced by cisplatin.
Abstract
The water extract of Younggyechulgam-tang (YGCGT) has been traditionally used in treatment of tinnitus in Oriental Medicine. However, little is known about the mechanism by which YGCGT rescues ototoxicity. The purpose of the present study is to investigate the protective effect of YGCGT against cisplatin-induced toxicity in HEI-OC1 auditory cells, organotypic cultures of cochlear explants from three-day postnatal rats (P3). Pretreatment with YGCGT ameliorated apoptotic death induced by cisplatin in HEI-OC1 cells and organotypic cultures of Corti’s organ. YGCGT pretreatment also significantly suppressed cisplatin-induced increases in intracellular reactive oxygen species (ROS). Treatment with YGCGT resulted in an increased expression of HO-1 and Bcl-2. These results suggest that YGCGT induced HO-1 signaling pathway, which ultimately prevents free radical stresses from cisplatin and further contributes to protect auditory sensory hair cells from free radicals produced by cisplatin.
참고문헌 (28)
- 張仲景, "仲景全書", pp.153~154 (1984), 대성문화사
- 신민교, "임상본초학", pp.172~519 (1986), 남산당
- 신태양사편집국, "원색최신의료대백과사전", pp.91~92 (1991), 신태양사
- 장인규, "痰飮의 원인․증상․치법에 관한 문헌적 고찰", 대한한의학회지, 7(1), pp.160~169 (1986)
- 巢元方, "諸病源候論校譯", pp.608~614 (1980), 人民衛生社
- Meyerhoff, "Diagnosis and management of hearing loss", pp.95~104 (1984), WB Saunders
- 정연훈, "이명에 대한 Glutamate 길항제의 치료효과", 대한이비인후과학회지 두경부외과학, 46(11), pp.935~939 (2003), 대한이비인후과학회
- 張介賓, "景岳全書", pp.477~481 (1970), 大聯國風出版社
- 許浚, "東醫寶鑑", pp.90~233 (1980), 南山堂
- 노석선, "안이비인후과학", pp.443~ (2003), 주민출판사
- 牛耕, "黃帝內經素問(2)", pp.314~ (2001), 여강출판사
- 張介賓, "國譯景岳全書(1)", pp.921~ (1993), 一中社
- 李梴., "醫學入門", pp.420~ (2006), 法仁文化社
- 오천민, "精校醫學正傳", pp.424~ (1973), 醫藥社
- 張錫純, "哀中參西錄(下卷)", pp.162~164 (1977), 輪成社
- 槄葉文禮, "腹證奇覽", pp.107~109 (1975), 癸丑文化社
- Laurell, G., "Ototoxic effect of cisplatin on guinea pigs in relation to dosage", Hear Res, 38(1), pp.27~33 (1989)
- Jordan, P., "Carmo-Fonesca, M. Molecular mechanism involved in cisplatin cytotoxicity", Cell Mol Life Sci, 57(8), pp.1229~1235 (2000)
- Lau, A. H., "Apoptosis induced by cisplatin nephrotoxic injury. Kidney Laurell G. Ototoxicity of the anticancer drug cisplatin. Clinical and experimental aspects", Scand Audio, (Suppl. 33), pp.1~47 (1991)
- Berman, S. B., "Dopamine oxidation alters mitochondrial respiration and induces permeability transition in brain mitochondria, implications for Parkinson’s disease", J Neurochem, 73, pp.1127~1137 (1999)
- Halestrap, A. P., "Mitochondria and cell death", Biochem Soc Trans, 28, pp.170~177 (2000)
- Tournier, C., "Requirement of JNK for stress-induced activation of the cytochrome c-mediated death pathway", Science, 288, pp.870~874 (2000)
- Maalouf, M., "Ketones inhibit mitochondrial production of reactive oxygen species production following glutamate excitotoxicity by increasing NADH oxidation", Neuroscience, 145, pp.256~264 (2007)
- Morgan, M. J., "Lipid rafts and oxidative stress induced cell death", Antioxidants Redox Signal, 9, pp.1471~1484 (2007)
- Fang, J., "Antiapoptotic role of heme oxygenase (HO) and the potential of HO as a target in anticancer treatment", Apoptosis, 9, pp.27~35 (2004)
- Doi, K., "Induction of hemoxygenase-1 by nitric oxide and ischaemia in experimental solid tumors and implications for tumour growth", Br J Cancer, 80, pp.1945~1954 (1999)
- Agarwal, A., "Induction of heme oxygenase in toxic renal injury : a protective role in cisplatin nephrotoxicity in the rat", Kidney Int, 48, pp.1298~1307 (1995)
- Yamasaki, F., "Apoptotic index in ovarian carcinoma : correlation with clinicopathologic factors and prognosis", Gynecol Oncol, 66(3), pp.439~448 (1997)