연자육이 음경해면체 이완에 미치는 영향
Relaxation Effects of Nelumbinis Semen in Isolated Corpus Cavernosum
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초록
This study was conducted to investigate the relaxation effects and its mechanisms of Nelumbinis Semen(NS) extract in isolated rabbit corpus cavernous tissues. In order to examine the relaxation effects and its mechanisms of NS, we treated the ethanol extract of NS(0.01-3.0 ㎎/㎖) and indomethacin(IM), tetraethylammonium chloride(TEA), Nω-nitro-L-arginine (L-NNA), methylene blue(MB) were treated before NS extract to contracted strips induced by PE 1 µM. We also treated calcium chloride(Ca) 1 mM after pretreatment of NS extract in Ca2+-free krebs-ringer solution to contracted strips induced by PE. Cell viability and NO concentration on human umbilical vein endothelial cell(HUVEC) was measured by MTT assay, Griess reagent system. eNOS production was investigated by histochemical and immunohistochemical staining. NS extract was significantly affected on the relaxation of cavernous strips and NS extract-induced relaxation was not different by pretreatment of IM, TEA, MB, but inhibited by the pretreatment of L-NNA. And increase of contraction induced by Ca2+ addition, in a Ca2+-free solution, was decreased by pretreatment of NS. NO concentration on HUVEC was increased. When NS extract was applicated on corpus cavernosum of penis(CCP) in SHR, ratio of smooth muscles to collage fibers by PE was decreased and formation of eNOS around helicine artery was increased. These results suggest that CCP relaxation effects of NS extract are shown by suppressing influx of extracellular Ca2+ through the production of NO and eNOS.
Abstract
This study was conducted to investigate the relaxation effects and its mechanisms of Nelumbinis Semen(NS) extract in isolated rabbit corpus cavernous tissues. In order to examine the relaxation effects and its mechanisms of NS, we treated the ethanol extract of NS(0.01-3.0 ㎎/㎖) and indomethacin(IM), tetraethylammonium chloride(TEA), Nω-nitro-L-arginine (L-NNA), methylene blue(MB) were treated before NS extract to contracted strips induced by PE 1 µM. We also treated calcium chloride(Ca) 1 mM after pretreatment of NS extract in Ca2+-free krebs-ringer solution to contracted strips induced by PE. Cell viability and NO concentration on human umbilical vein endothelial cell(HUVEC) was measured by MTT assay, Griess reagent system. eNOS production was investigated by histochemical and immunohistochemical staining. NS extract was significantly affected on the relaxation of cavernous strips and NS extract-induced relaxation was not different by pretreatment of IM, TEA, MB, but inhibited by the pretreatment of L-NNA. And increase of contraction induced by Ca2+ addition, in a Ca2+-free solution, was decreased by pretreatment of NS. NO concentration on HUVEC was increased. When NS extract was applicated on corpus cavernosum of penis(CCP) in SHR, ratio of smooth muscles to collage fibers by PE was decreased and formation of eNOS around helicine artery was increased. These results suggest that CCP relaxation effects of NS extract are shown by suppressing influx of extracellular Ca2+ through the production of NO and eNOS.
참고문헌 (40)
- Kim, J. H., "Experimental hypercholesterolemia in rabbits induces cavernosal atherosclerosis with endothelial and smooth muscle cell dysfunction", Journal of Urology, 151(1), pp.198~205 (1994)
- Azadzoi, K. M., "Hypercholesterolemia impairs endothelium-dependent relaxation of rabbit corpus cavernosum smooth muscle", Journal of Urology, 146(1), pp.238~240 (1991)
- 권강범, "加味馬錢五子丸 추출물이 雄性 白鼠의 성기능에 미치는 효과", 동의생리병리학회지, 18(5), pp.1410~1417 (2004), 한의병리학회
- Lee, H.Y., "Andrology", pp.232~ (1987), Publishing department of Seoul National University
- Maas, R., "The pathophysiology of erectile dysfunction related to endothelial dysfunction and mediators of vascular function", Vascular Medicine, 7(3), pp.213~225 (2002)
- Kim, S.K., "Urology" (1991), Korea Medical Book Publisher
- Urology of Oriental Medicine, "Doo, H.K.", Institute of Oriental medicine, 1152~ (1992)
- Shin, M.K., "Clinic Herbology", pp.505~ (1997), Younglim press
- The Jointly Published Textbook Compilation Committee of Oriental College of Medicine Nationwide Department, "Herbology", pp.684~ (2004), Younglim press
- Birgitte, L. C., "Traditional chinese medicine in treatment of hyperlipidaemia", Journal of Ethnopharmacology, 46, pp.125~159 (1995)
- Jong-Hoon Kim, "Effects of Nelumbinis Semen on Contractile Dysfunction in Ischemic and Reperfused Rat Heart", Archives of Pharmacal Research, 29(9), pp.777~785 (2006), 대한약학회
- Effects of Nelumbinis semen on LPS-induced NO production by BV2 microglial cells, "Kim, T. H", Graduate School of Kyunghee University, ~ (2006)
- 박재호, "연자육 추출물의 항산화 및 산화적 DNA 손상억제 활성", 대한본초학회지, 25(4), pp.55~59 (2010), 대한본초학회
- Mukherjee, P. K., "Hypoglycemic activity of Melumbo nuficera rhizome(methabolic extract) in streptozoticin induced diabetic rats", Phytotherapy Research, 9, pp.522~524 (1995)
- Mukherjee, P.K., "Antidiarrhoeal evaluation of Nelumbo nuficera rhizome extract", Indian Journal of Pharmacology, 27, pp.262~364 (1995)
- Mukherjee, P. K., "Antifungal screening of Nelumbo nuficera (Nymphaeaceae) rhizome extract", Indian Journal of Pharmacology, 35, pp.327~330 (1995)
- Mukherjee, P. K., "Antibacterial efficiency of Nelumbo nuficera(Nymphaeaceae) rhizome extract", Indian Drugs, 32, pp.274~276 (1995)
- Song, G. S., "Effect of hot water extracts from medical plants on the mutagenicity of indirect mutagens", Journal of Food Science and Technology, 29, pp.1288~1294 (1997)
- 이진우, "연자육의 항우울 효과 및 프로티옴 분석을 통한 기전 연구", 동의생리병리학회지, 20(4), pp.830~843 (2006), 한의병리학회
- 장춘곤, "Nelumbinis Semen Reverses a Decrease in 5-HT1A Receptor Binding Induced by Chronic Mild Stress, a Depression-like Symptom", Archives of Pharmacal Research, 27(10), pp.1065~1072 (2004), 대한약학회
- Kang, M. K., "The antidepressant effect of Melumbinis Semen on rats under chronic mild stress induced depression-like symptoms", The American Journal of Chinese Medicine, 33, pp.205~213 (2005)
- 이준영, "연자육 추출물의 멜라닌 합성 저해효과", KSBB Journal, 28(2), pp.137~145 (2013), 한국생물공학회
- Carmichael, J., "Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing", Cancer Research, 47, pp.936~942 (1987)
- Tracey, W. R., "Comparison of spectrophotometric and biological assay for nitric oxide and endothelium-derived relaxing factor(EDRF) : Neurospecificity of the diazotiazation reaction for NO and failure to detect EDRF", Journal of Pharmacology, 252, pp.922~928 (1990)
- Steers, W. D., "Some pharmacologic effects of yohimbine on human and rabbit penis", Journal of Urology, 131(4), pp.799~802 (1984)
- Palmer, R. M. J., "Vascular endothelial cells synthesize nitric oxide from L-arginine", Nature, 333, pp.664~666 (1989)
- Stamler, J. S., "Biochemistry of nitric oxide and its redox activated forms", Science, 258, pp.1898~1902 (1992)
- De May, J. G., "Rolo of the intima in cholinergic and purinergic relaxation of isolated canin femoral arteries", Journal of Physiology, 316, pp.347~355 (1981)
- Moody, J. A., "Effects of long-term oral administration of L-arginine on the rat erectile response", Journal of Urology, 158, pp.942~947 (1997)
- Chen, J., "Effect of oral administration of high-dose nitric oxide donor L-arginine in men with organic erectile dysfunction: result of a double-blind, randomized, placebo-controlled study", British Journal of Urology International, 83, pp.269~273 (1999)
- 리향, "석창포에 의한 발기부전 개선 효과", 동의생리병리학회지, 25(5), pp.863~869 (2011), 한의병리학회
- 백기범, "Ethanol로 발기부전을 유도한 흰쥐의 성기능 개선에 蜈蚣추출물이 미치는 영향", 동의생리병리학회지, 22(1), pp.176~182 (2008), 한의병리학회
- Son, H. J., "Effects of the extract fo Rubi Fructus on urethral nitric oxide synthase activity and nitrite level in chronic ethanol –treated rats", The Journal of the Korea Institute of Oriental Medical Informatics, 6(1), pp.46~56 (2000)
- Lee, S. W., "Prostaglandin E1 activates the large-conductance KCa channel in human corporal smooth muscle cells", International Journal of Impotence Research, 11, pp.189~199 (1999)
- Lee, S. W., "Characterization of ATP-sensitive potassium channels in human corporal smooth muscle cells", International Journal of Impotence Research, 11, pp.179~188 (1999)
- Ko, F. N., "Vasodilatory action mechanism of apigenin isolated from Apium graveolens in rat thoracic aorta.", Biochemica et Biophysica Acta, 1115, pp.69~74 (1991)
- Akingba, A. G., "Endothilial nitric oxide synthase protein expression, localization, and activity in the penis of the alloxan-induced diabetic rat", Molecular Urology, 5, pp.189~197 (2001)
- Gonzalez, C. M., "Protein and gene expression of nitric oxide synthase isoforms Ⅰand Ⅲ in the rat penile shaft", Journal of Andrology, 22, pp.54~61 (2001)
- Chen, J., "Effect of oral administration of high-dose nitric oxide donor L-arginine in men with organic erectile dysfunction: result of a double-blind, randomized, placebo-controlled study", British Journal of Urology International, 83, pp.269~273 (1999)
- Angulo, J., "Activation and potentiation of the NO", cGMP pathway by NG-hydroxyl-L-arginine in rabbit corpus cavernosum under normoxic and hypoxic conditions and ageing, 2003, pp.138, 63~70