Expression of MUC5AC and Trefoil Peptide 1 (TFF1) in the Subtypes of Intestinal Metaplasia
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background/Aims: Alterations of the expression pattern of mucins and trefoil peptides have been described in gastric adenocarcinomas and in their precursor lesions. The aim of this study was to determine the progression patterns of intestinal metaplasia (IM) subtypes by analyzing the expression patterns of TFF1 and MUC5AC in different subtypes of IM of the stomach. Methods: Endoscopic gastric biopsies of the antrum and body were obtained from patients with dyspepsia and endoscopic IM. Alcian blue/periodic acid-Schiff staining and the high iron diamine technique were used to classify the subtypes of IM. Immunoreactivity for MUC5AC and TFF1 was estimated in different types of IM. Results: IM was detected in 128 samples from 80 patients; type I was found in 48 samples, type II was found in 37 samples, and type III was found in 43 samples. There was a gradual decrease in MUC5AC and TFF1 expression during the progression of IM from type I to type III via the type II intermediate. Conclusions: This downregulation of MUC5AC and TFF1 expression may challenge the sequential progression of IM from type I to type III via the type II intermediate, and it might be associated with gastric carcinogenesis.
Abstract
Background/Aims: Alterations of the expression pattern of mucins and trefoil peptides have been described in gastric adenocarcinomas and in their precursor lesions. The aim of this study was to determine the progression patterns of intestinal metaplasia (IM) subtypes by analyzing the expression patterns of TFF1 and MUC5AC in different subtypes of IM of the stomach. Methods: Endoscopic gastric biopsies of the antrum and body were obtained from patients with dyspepsia and endoscopic IM. Alcian blue/periodic acid-Schiff staining and the high iron diamine technique were used to classify the subtypes of IM. Immunoreactivity for MUC5AC and TFF1 was estimated in different types of IM. Results: IM was detected in 128 samples from 80 patients; type I was found in 48 samples, type II was found in 37 samples, and type III was found in 43 samples. There was a gradual decrease in MUC5AC and TFF1 expression during the progression of IM from type I to type III via the type II intermediate. Conclusions: This downregulation of MUC5AC and TFF1 expression may challenge the sequential progression of IM from type I to type III via the type II intermediate, and it might be associated with gastric carcinogenesis.
참고문헌 (16)
- Leung WK, "Review article: intestinal metaplasia and gastric carcinogenesis", Aliment Pharmacol Ther, 16, pp.1209~1216 (2002)
- Filipe MI, "Intestinal metaplasia sub-types and cancer risk, In Gastric Carcinoma", pp.87~115 (1986), Churchill Livingstone
- Silva S, "Intestinal metaplasia and its variants in the gastric mucosa of Portuguese subjects: a comparative analysis of biopsy and gastrectomy material", Hum Pathol, 17, pp.988~995 (1986)
- Filipe MI, "Intestinal metaplasia types and the risk of gastric cancer: a cohort study in Slovenia", Int J Cancer, 57, pp.324~329 (1994)
- Sands BE, "The trefoil peptide family", Annu Rev Physiol, 58, pp.253~273 (1996)
- Kim BW, "Expression of trefoil peptides in the subtypes of intestinal metaplasia", Peptides, 25, pp.779~783 (2004)
- Filipe MI, "Appendix 3, In Histochemistry in Pathology. 2nd ed", pp.449~453 (1990), Churchill Livingsto
- Corfield AP, "Mucins and mucosal protection in the gastrointestinal tract: new prospects for mucins in the pathology of gastrointestinal disease", Gut, 47, pp.589~594 (2000)
- Carrato C, "Differential apomucin expression in normal and neoplastic human gastrointestinal tissues", Gastroenterology, 107, pp.160~172 (1994)
- Longman RJ, "Coordinated localisation of mucins and trefoil peptides in the ulcer associated cell lineage and the gastrointestinal mucosa", Gut, 47, pp.792~800 (2000)
- Beckler AD, "Decreased abundance of trefoil factor 1 transcript in the majority of gastric carcinomas", Cancer, 98, pp.2184~2191 (2003)
- Taupin D, "Augmented intestinal trefoil factor (TFF3) and loss of pS2 (TFF1) expression precedes metaplastic differentiation of gastric epithelium", Lab Invest, 81, pp.397~408 (2001)
- Ruchaud-Sparagano MH, "The trefoil protein TFF1 is bound to MUC5AC in human gastric mucosa", Cell Mol Life Sci, 61, pp.1946~1954 (2004)
- Machado JC, "Gastric carcinoma exhibits distinct types of cell differentiation: an immunohistochemical study of trefoil peptides (TFF1 and TFF2) and mucins (MUC1, MUC2, MUC5AC, and MUC6)", J Pathol, 190, pp.437~443 (2000)
- Suriani R, "Type III intestinal metaplasia, Helicobacter pylori infection and gastric carcinoma risk index in an Italian series of 1750 patients", Hepatogastroenterology, 52, pp.285~288 (2005)
- Smith JL, "Is subtyping of intestinal metaplasia in the upper gastrointestinal tract a worthwhile exercise? An evaluation of current mucin histochemical stains", Br J Biomed Sci, 60, pp.180~186 (2003)