콜라겐 분해효소를 이용한 뇌출혈 백서 모델에서의 신경학적 결손 평가를 위한 행동 검사
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Department of Neurology, College of medicine Seoul National University Department of Neurology, Seoul National Hospital* Department of Neurology, Ilsan Paik Hospital, Inje University** Background: Experimental intracerebral hemorrhage (ICH) in rats made by collagenase, causes focal, asymmetrical neurologic deficits. However, the time course of the neurologic deficit in these models have not been quantified yet. Variable behavioral tests without verification are being used. Objectives: To evaluate the time course of the sensorimotor deficits in experimental ICH and to examine the consistency of variable behavioral tests. Methods: Experimental ICH was induced by stereotactic, intrastriatal administration of bacterial collagenase in adult male, Sprague-Dawley rats (weight, 200-220 g, n=13). Control had only a needle insertion (SHAM group, n=5). Rats were tested before ICH, and after ICH, from 1 day to 35 days (every week) using modified limb placing test, cylinder test, swimming test, and rotarod test. Results: Rats with ICH had marked neurologic deficits from day 1, which remained persistent up to 5 weeks after ICH, in Rotarod test, modified limb placing test, cylinder test, and swimming test. Initial behavioral scores were below 50 percent of that of the pre-ICH status, and the neurologic deficits occurred in the bilateral sides initially. The abnormal neurologic score ipsilateral to the ICH side, improved over time. Some rats of the SHAM group showed the asymmetry in cylinder test, modified limb placing test and swimming test. Conclusions: In this study, experimental ICH caused long-term functional changes in rats. Some functional improvement and the neurologic deficit from needle insertion itself should be considered in the use of this model.
Abstract
Department of Neurology, College of medicine Seoul National University Department of Neurology, Seoul National Hospital* Department of Neurology, Ilsan Paik Hospital, Inje University** Background: Experimental intracerebral hemorrhage (ICH) in rats made by collagenase, causes focal, asymmetrical neurologic deficits. However, the time course of the neurologic deficit in these models have not been quantified yet. Variable behavioral tests without verification are being used. Objectives: To evaluate the time course of the sensorimotor deficits in experimental ICH and to examine the consistency of variable behavioral tests. Methods: Experimental ICH was induced by stereotactic, intrastriatal administration of bacterial collagenase in adult male, Sprague-Dawley rats (weight, 200-220 g, n=13). Control had only a needle insertion (SHAM group, n=5). Rats were tested before ICH, and after ICH, from 1 day to 35 days (every week) using modified limb placing test, cylinder test, swimming test, and rotarod test. Results: Rats with ICH had marked neurologic deficits from day 1, which remained persistent up to 5 weeks after ICH, in Rotarod test, modified limb placing test, cylinder test, and swimming test. Initial behavioral scores were below 50 percent of that of the pre-ICH status, and the neurologic deficits occurred in the bilateral sides initially. The abnormal neurologic score ipsilateral to the ICH side, improved over time. Some rats of the SHAM group showed the asymmetry in cylinder test, modified limb placing test and swimming test. Conclusions: In this study, experimental ICH caused long-term functional changes in rats. Some functional improvement and the neurologic deficit from needle insertion itself should be considered in the use of this model.