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Influence of Combined Methionine Synthase (MTR 2756A>G) and Methylenetetrahydrofolate Reductase (MTHFR 677C>T) Polymorphisms to Plasma Homocysteine Levels in Korean Patients with Ischemic Stroke

오도연|김남근|황태선|홍선표|안정용|김수옥|유왕돈|홍승호|김옥준
연세대학교의과대학 (Yonsei University College of Medicine)|2007.04|Yonsei Medical Journal|제48권 제2호|pp. 201-209

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초록

Abstract

Purpose: Methionine synthase (MTR) and 5,10-methylenetetrahydrofolate reductase (MTHFR) are the main regulatory enzymes for homocysteine metabolism. The present case- control study was conducted to determine whether there is an association between the MTR 2756A>G or MTHFR 677C >T polymorphism and plasma homocysteine concentration in Korean subjects with ischemic stroke. Materials and Methods: DNA samples of 237 patients who had an ischemic stroke and 223 age and sex-matched controls were studied. MTR 2756A>G and MTHFR 677C>T genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: Frequencies of mutant alleles for MTR and MTHFR polymorphisms were not significantly different between the controls and cases. The patient group, however, had significantly higher homocysteine concentrations of the MTR 2756AA and MTHFR 677TT genotypes than the control group (p=0.04 for MTR, p=0.01 for MTHFR). The combined MTR 2756AA and MTHFR 677TT genotype (p= 0.04) and the homocysteine concentrations of the patient group were also higher than those of the controls. In addition, the genotype distribution was significant in the MTHFR 677TT genotype (p=0.008) and combined MTR 2756AA and MTHFR 677TT genotype (p=0.03), which divided the groups into the top 20% and bottom 20% based on their homocysteine levels. Conclusion: The results of the present study demonstrate that the MTR 2756A>G and MTHFR 677C>T polymorphisms interact with elevated total homocysteine (tHcy) levels, leading to an increased risk of ischemic stroke.

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관련 논문AI추천

뇌경색 환자에서 고호모시스테인혈증과 C677T MTHER 유전자 다형성의 상관 관계김남근, 김상범, 김선희, 김옥준, 김현조, 서정호, 오도연, 이규용, 정경천, 정보우, 최병옥대한신경과학회 · 대한신경과학회지2002 · 제20권 제4호MTHFR C677T 유전자 다형성과 허혈성 뇌졸중의 상관 관계김숙희, 김지영, 김희진, 박기덕, 윤지영, 이정화, 조현지, 최경규, 최병옥대한신경과학회 · 대한신경과학회지2005 · 제23권 제5호Association of Methylenetetrahydrofolate Reductase (MTHFR 677C>T and 1298A>C) Polymorphisms and Haplotypes with Silent Brain Infarction and Homocysteine Levels in a Korean Population허륭, 김남근, 김옥준, 안정용, 오도연, 정상섭, 한인보, 홍순표연세대학교의과대학 · Yonsei Medical Journal2010 · 제51권 제2호허혈성 뇌졸중 환자에서 대사증후군과 호모시스테인의 관계박종호, 박현미, 신동진, 신원철, 이영배대한신경과학회 · 대한신경과학회지2005 · 제23권 제1호MTHFR C677T 유전자 다형성과 다발성 뇌경색 환자군에서의 비교위험도 분석이규용대한뇌졸중학회 · 대한뇌졸중영문학회지2002 · 제4권 제1호허혈성 뇌병변이 발견된 고호모시스테인혈증과 동반된 비타민B12 결핍권혜미, 길영은, 서홍일, 이선민, 임태성대한신경과학회 · 대한신경과학회지2017 · 제35권 제4호

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