Identification of Up-Regulated Genes in Malignant Glioma with Subtraction Hybridization: Preliminary Screening Studies
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Purpose: This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization. Materials and Methods: Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the GenbankTM database. Results: Using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line. Conclusion: Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.
Abstract
Purpose: This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization. Materials and Methods: Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the GenbankTM database. Results: Using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line. Conclusion: Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.
참고문헌 (22)
- Dai C, "Astrocyte differentiation states and gliomas formation", Cancer J, 9, pp.72~81 (2003)
- Sallinen SL, "Identification of differentially expressed genes in human gliomas by DNA microarray and tissue chip techniques", Cancer Res, 60, pp.6617~6622 (2000)
- Christensen HC, "Incidences of gliomas and meningiomas in Denmark, 1943 to 1997", Neurosurgery, 52, pp.1327~1333 (2003)
- Velculescu VE, "Characterization of the yeast transcriptome", Cell, 88, pp.243~251 (1997)
- Chook YM, "Karyopherins and nuclear import", Curr Opin Struct Biol, 1, pp.703~715 (2001)
- Nutt CL, "Brain enriched hyaluronan binding (BEHAB)", brevican increases aggressiveness of CNS-1 gliomas in Lewis rats, 2001, pp.61, 7056~7059
- Palackal NT, "Activation of polycyclic aromatic hydrocarbon trans-dihydrodiol proximate carcinogens by human aldo-keto reductase (AKR1C) enzymes and their functional overexpression in human lung carcinoma (A549) cells", J Biol Chem, 277, pp.24799~24808 (2002)
- Rehman I, "(Calcyclin) is a prostate basal cell marker absent in prostate cancer and its precursors", Br J Cancer, 91, pp.739~744 (2004)
- Welsh J, "Arbitrarily primed PCR fingerprinting of RNA", Nucleic Acids Res, 20, pp.4965~4970 (1992)
- Smith JM, "HMG box transcription factor gene Hbp1 is expressed in germ cells of the developing mouse testis", Dev Dyn, 230, pp.366~370 (2004)
- van Nocker S, "The WD-repeat protein superfamily in Arabidopsis: conservation and divergence in structure and function", BMC Genomics, 4, pp.50~ (2003)
- Porkka KP, "Detection of differentially expressed genes in prostate cancer by combining suppression subtractive hybridization and cDNA library array", J Pathol, 193, pp.73~79 (2001)
- Wandrey M, "Molecular and cell biology of a family of voltage- dependent anion channel porins in Lotus japonicus", Plant Physiol, 134, pp.182~193 (2004)
- Zeindl-Eberhart E, "Detection and identification of tumor-associated protein variants in human hepatocellular carcinomas", Hepatology, 39, pp.540~549 (2004)
- Diatchenko L, "Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries", Proc Natl Acad Sci U S A, 93, pp.6025~6030 (1996)
- Hufton SE, "A profile of differentially expressed genes in primary colorectal cancer using suppression subtractive hybridization", FEBS Lett, 463, pp.77~82 (1999)
- Ji W, "Efficacy of SSH PCR in isolating differentially expressed genes", BMC Genomics, 3, pp.12~ (2002)
- Lee SJ, "Molecular cloning of a novel pathogen-inducible cDNA encoding a putative acyl-CoA synthetase from Capsicum annuum L", Plant Mol Biol, 46, pp.661~671 (2001)
- Shridhar V, "Identification of underexpressed genes in early- and late-stage primary ovarian tumors by suppression subtraction hybridization", Cancer Res, 62, pp.262~270 (2002)
- Swearingen ML, "Detection of differentially expressed HES-6 gene in metastatic colon carcinoma by combination of suppression subtractive hybridization and cDNA library array", Cancer Lett, 198, pp.229~239 (2003)
- Liang P, "Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction", Science, 257, pp.967~971 (1992)
- Dong Y, "Ip C. Delineation of the molecular basis for selenium- induced growth arrest in human prostate cancer cells by oligonucleotide array", Cancer Res, 63, pp.52~59 (2003)