Matrine Suppresses Pancreatic Fibrosis by Regulating TGF-β/Smad Signaling in Rats
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Purpose: This study aimed to elucidate the molecular mechanisms of the anti-pancreatic fibrosis effects of matrine in rats. Materials and Methods: Trinitrobenzene sulfonic acid was administrated to rats to establish a pancreatic fibrosis model. Rats weredivided into four groups: Control, Sham, Model, and Matrine (n=8). Hematoxylin-eosin staining, Masson staining, and Azan stainingwere performed to evaluate pancreatic fibrosis. Expression of transforming growth factor-β1 (TGF-β1), α-smooth muscle actin(α-SMA), and collagen I in pancreatic tissues was evaluated by immunohistochemical staining. mRNA and protein levels of TGF-βreceptor 1 (TβR1), TβR2, and Smad2 in pancreatic tissues were determined by RT-PCR and Western blot, respectively. Results: In the model group, hyperplasia of glandules around the glandular ducts, mitochondrial swelling of acinous cells, andsevere fibrosis were found. Interestingly, in the Matrine group, mitochondrial swelling was only found in a small number of acinouscells, and the fundamental structures of pancreatic tissues were intact. Moreover, pancreatic fibrosis was markedly alleviated. Comparing to the Sham group, expression of α-SMA, TGF-β1, and collagen I was sharply elevated in the Model group (p<0.05);however, their expressions were much lower in the Matrine group, compared to the Model group (p<0.05). Compared with theSham group, mRNA and protein levels of Smad2, TβR1, and TβR2 in the Model group were notably raised (p<0.05). However,their high expression was significantly downregulated in the Matrine group (p<0.05). Conclusion: Matrine suppressed pancreatic fibrosis by regulating TGF-β/Smad signaling in rats.
Abstract
Purpose: This study aimed to elucidate the molecular mechanisms of the anti-pancreatic fibrosis effects of matrine in rats. Materials and Methods: Trinitrobenzene sulfonic acid was administrated to rats to establish a pancreatic fibrosis model. Rats weredivided into four groups: Control, Sham, Model, and Matrine (n=8). Hematoxylin-eosin staining, Masson staining, and Azan stainingwere performed to evaluate pancreatic fibrosis. Expression of transforming growth factor-β1 (TGF-β1), α-smooth muscle actin(α-SMA), and collagen I in pancreatic tissues was evaluated by immunohistochemical staining. mRNA and protein levels of TGF-βreceptor 1 (TβR1), TβR2, and Smad2 in pancreatic tissues were determined by RT-PCR and Western blot, respectively. Results: In the model group, hyperplasia of glandules around the glandular ducts, mitochondrial swelling of acinous cells, andsevere fibrosis were found. Interestingly, in the Matrine group, mitochondrial swelling was only found in a small number of acinouscells, and the fundamental structures of pancreatic tissues were intact. Moreover, pancreatic fibrosis was markedly alleviated. Comparing to the Sham group, expression of α-SMA, TGF-β1, and collagen I was sharply elevated in the Model group (p<0.05);however, their expressions were much lower in the Matrine group, compared to the Model group (p<0.05). Compared with theSham group, mRNA and protein levels of Smad2, TβR1, and TβR2 in the Model group were notably raised (p<0.05). However,their high expression was significantly downregulated in the Matrine group (p<0.05). Conclusion: Matrine suppressed pancreatic fibrosis by regulating TGF-β/Smad signaling in rats.
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