Serum microRNA-381: A Potential Marker for Early Diagnosis of Gastric Cancer
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Purpose: The purpose of this study was to explore the potential early diagnostic value of serum microRNA-381(miRNA-381) inpatients with gastric cancer (GC). Materials and Methods: Patients with advanced gastric cancer (AGC) and early gastric cancer (EGC), as well as healthy individuals,were enrolled in this study. Expression of miRNA-381 in serum was detected using real-time quantitative PCR. Electrochemiluminescenceanalysis was used to investigate the expression of classic tumor markers, including carbohydrate antigen (CA) 199,CA724, and carcinoembryonic antigen. Finally, receiver operating characteristic curve and Kaplan-Meier analysis were used todetermine the value of miRNA-381 in clinical diagnosis of GC. Results: miRNA-381 was differentially expressed among the study groups. AUC analysis showed that the sensitivity and specificityof serum miRNA-381 in the diagnosis of GC were superior to those of other tumor markers. Furthermore, low levels of miRNA-381 expression were positively correlated with lymph node metastasis and AGC. Finally, Kaplan-Meier survival analysisshowed that down-regulation of miRNA-381 was associated with lymph node metastasis and the development of GC. Conclusion: miRNA381, which was down-regulated in GC, might be a novel early diagnosis marker for patients with GC.
Abstract
Purpose: The purpose of this study was to explore the potential early diagnostic value of serum microRNA-381(miRNA-381) inpatients with gastric cancer (GC). Materials and Methods: Patients with advanced gastric cancer (AGC) and early gastric cancer (EGC), as well as healthy individuals,were enrolled in this study. Expression of miRNA-381 in serum was detected using real-time quantitative PCR. Electrochemiluminescenceanalysis was used to investigate the expression of classic tumor markers, including carbohydrate antigen (CA) 199,CA724, and carcinoembryonic antigen. Finally, receiver operating characteristic curve and Kaplan-Meier analysis were used todetermine the value of miRNA-381 in clinical diagnosis of GC. Results: miRNA-381 was differentially expressed among the study groups. AUC analysis showed that the sensitivity and specificityof serum miRNA-381 in the diagnosis of GC were superior to those of other tumor markers. Furthermore, low levels of miRNA-381 expression were positively correlated with lymph node metastasis and AGC. Finally, Kaplan-Meier survival analysisshowed that down-regulation of miRNA-381 was associated with lymph node metastasis and the development of GC. Conclusion: miRNA381, which was down-regulated in GC, might be a novel early diagnosis marker for patients with GC.
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