LC/MS를 이용한 heterocyclic amines의 분석법

Abstract

The heterocyclic amines (HAs) are a family of mutagenic/carcinogenic chemicals formed from thefoods is their very low level of concentration (ng/g) and a number of matrix interferences in samples. Solid-phaseextraction (SPE) is one of the procedures widely used for the extraction and purification of HAs in fod samples.In this study, several SPE procedures of HAs determination were performed. Recoveries of the HAs were obtainedfrom comparing a matrix such as a standard methanolic solution and pre-cooked meat extracts. Recovery valueswere ranging between 25.3 and 93.0 % at a concentration of 25.0 ng/g. HAs were determined with high sensitivityby micro-HPLC (-HPLC) analysis with electrospray ionization mass spectrometry (ESI-MS). Another developedmethod, which is freezing filtration method, shows better extraction recoveries and good precisions. The establishedmethod wil be applicable to monitoring of heterocyclic amines from the cooked meat.