Separation of liquiritin, glycyrrhizic acid and glabridin from licorice by RP - HPLC
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초록
Reversed-phase high performance liquid chromatography (RP-HPLC) was used for the simultaneous determination of liquiritin (LQ), glycyrrhizic acid (GA) and glabridin from licorice. An optimized run condition was selected with a binary gradient elution of methanol-water which ramped 35/65 to 80/20 (vol. %) in 0.0- 8.0 min and a flow rate of 1.0 mL/min. A good linearity was obtained between 0.2 mg/mL and 1.0 mg/mL for LQ and GA, and 0.01 mg/mL-0.2 mg/mL for glabridin with the relative standard deviations less than 0.90% (n=5). The developed method was successfully applied to determination of the three components from licorice samples. The mean recoveries of three components are 80.79% for liquiritin, 89.71% for glycyrrhizic acid and 72.50% for glabridin.
Abstract
Reversed-phase high performance liquid chromatography (RP-HPLC) was used for the simultaneous determination of liquiritin (LQ), glycyrrhizic acid (GA) and glabridin from licorice. An optimized run condition was selected with a binary gradient elution of methanol-water which ramped 35/65 to 80/20 (vol. %) in 0.0- 8.0 min and a flow rate of 1.0 mL/min. A good linearity was obtained between 0.2 mg/mL and 1.0 mg/mL for LQ and GA, and 0.01 mg/mL-0.2 mg/mL for glabridin with the relative standard deviations less than 0.90% (n=5). The developed method was successfully applied to determination of the three components from licorice samples. The mean recoveries of three components are 80.79% for liquiritin, 89.71% for glycyrrhizic acid and 72.50% for glabridin.
참고문헌 (16)
- F. Aoki, , J. Chromatogr. B, 828, pp.70~74 (2005)
- H. Liu, , Anal. Chimica Acta, 458, pp.345~354 (2002)
- Y. W. Kim, , Chemico-Bio. Interactions, 161, pp.125~138 (2006)
- J. Vaya, , Free Radical Bio. Med, 23, pp.302~313 (1997)
- T. Hatano, , Chem. Pharm.Bull, 36, pp.2286~2288 (1988)
- J. Cong, , J. Chromatogr. A, 1145, pp.190~194 (2007)
- S. K. Acharya, , Indian J. Med. Res. B-Biomedical Research other than Infectious Diseases, 98, pp.69~74 (1993)
- T. Tanahashi, , J. Steroid Biochem. Molecular Biology, 80, pp.441~447 (2002)
- Y. Fujisawa, , Microbiology and Immunology, 44, pp.799~804 (2000)
- A. R. Dehpour, , International J. Pharmaceutics, 119, pp.133~138 (1995)
- J. Cinatl, , Lancet, 361, pp.2045~2046 (2003)
- B. Fu, , J. Chromatogr. A, 1089, pp.18~24 (2005)
- E. Choi, , Biochem. Pharmacology, 70, pp.363~368 (2005)
- S. Shen, , Sep. Purification Tech, 53, pp.216~223 (2007)
- C. Sun, , Colloids and Surfaces A: Physicochemical and Engineering Aspects, 305(1-3), pp.42~47 (2007)
- Y. Lee, , J. Chromatogr.A, 692, pp.137~145 (1995)