본문 바로가기
KERD
논문KCI 등재

동위원소희석 액체 크로마토그래피-질량분석법을 이용한 차음료 중의 소르빈산의 분석

Analysis of sorbic acid in tea-drink using isotope dilution liquid chromatography tandem mass spectrometry (ID-LC/MS/MS)

이인선|김병주|안성희
한국분석과학회|2012.02|분석과학 (Analytical Science & Technology)|제25권 제1호|pp. 25-32

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

sorbic acidreference materialsID-LC/MSexpanded uncertainty
주제분류화학

초록

Isotope dilution mass spectrometry (ID-MS) based on liquid chromatography tandem mass spectrometry (LC/MS/MS) was developed for the accurate determination of sorbic acid in tea-drink. An isotope analogue of sorbic acid, 13C2-sorbic acid, was obtained by custom synthesis. MS was operated in the negative mode with selected reaction monitoring (SRM) mode of [M-H]- → [M-CO2H]- channel at m/z 111 → 67 for sorbic acid and at m/z 113 → 68 for its isotope analogue. Chromatographic separation was accomplished with a C18column and an isocratic mobile phase of 55% of 50 mM ammonium acetate (pH 4.5) and 45% of methanol. Homogeneous reference materials were prepared for validation of this method, including repeatability and reproducibility tests, by fortifying tea-drink with sorbic acid in our laboratory. Repeatability and reproducibility studies showed that the ID-LC/MS method is a reliable and reproducible method which provides less than 3.8%of relative standard deviation (RSD) for the analysis of sorbic acid.

Abstract

Isotope dilution mass spectrometry (ID-MS) based on liquid chromatography tandem mass spectrometry (LC/MS/MS) was developed for the accurate determination of sorbic acid in tea-drink. An isotope analogue of sorbic acid, 13C2-sorbic acid, was obtained by custom synthesis. MS was operated in the negative mode with selected reaction monitoring (SRM) mode of [M-H]- → [M-CO2H]- channel at m/z 111 → 67 for sorbic acid and at m/z 113 → 68 for its isotope analogue. Chromatographic separation was accomplished with a C18column and an isocratic mobile phase of 55% of 50 mM ammonium acetate (pH 4.5) and 45% of methanol. Homogeneous reference materials were prepared for validation of this method, including repeatability and reproducibility tests, by fortifying tea-drink with sorbic acid in our laboratory. Repeatability and reproducibility studies showed that the ID-LC/MS method is a reliable and reproducible method which provides less than 3.8%of relative standard deviation (RSD) for the analysis of sorbic acid.

참고문헌 (31)

  1. P.E. Hartman, , Environ. Mutagen, 5, pp.217~222 (1983)
  2. R. Munzer, , Food Chem Toxicol, 28, pp.397~401 (1990)
  3. C. Ferrand, , Food addit. Contam, 17, pp.895~901 (2000)
  4. C. Ferrand, , J. Agric. Food Chem, 48, pp.3605~3610 (2000)
  5. D. Sochin, , J. Am. Acad. Dermatol, 14, pp.234~241 (1986)
  6. O. W. Lau, , Analyst, 114, pp.1269~1272 (1987)
  7. G. Wilamowski, , J. Assoc. Official Anal. Chem, 54, pp.663~665 (1971)
  8. O. W. Lau, , Analyst, 114, pp.1047~1051 (1989)
  9. T. Renner, , J. Chromatogr. A, 847, pp.127~133 (1999)
  10. N. Ochiai, , Anal. Bioanal. Chem, 373, pp.56~63 (2002)
  11. M. M. Villiers, , Drug Dev. and Ind. Pharm, 26, pp.539~547 (2000)
  12. I. M. P. L. V. O. Ferreira, , Food Res. Int, 33, pp.113~117 (2000)
  13. S. A. V. Tfouni, , Food Control, 13, pp.117~123 (2002)
  14. I. Garcia, , J. Chromatogr. A, 992, pp.11~27 (2003)
  15. F. J. M. Mota, , Food Chem, 82, pp.469~473 (2003)
  16. A. El-Gindy, , J. Pharm. Biomed. Analysis, 35, pp.703~713 (2004)
  17. S. Negri, , Chem.-Biol. Interact, 153, pp.243~246 (2005)
  18. B. Saad, , J. Chromatogr. A, 1073, pp.393~397 (2005)
  19. Z. Dobiasova, , Electrophoresis, 23, pp.263~267 (2002)
  20. Y. Tang, , Talanta, 65, pp.794~798 (2005)
  21. G. Dube, , Metrologia, 34, pp.83~86 (1997)
  22. P. de Bivere, , Anal. Proc, 30, pp.328~333 (1993)
  23. K. G. Heumann, , J. Mass Spectrom. Rev, 11, pp.41~67 (1992)
  24. A. P. de Lennheer, , Int. J. Mass Spectrom. Rev, 11, pp.249~307 (1992)
  25. V. Barwick, "Best Practice Guide for Generating Mass Spectra", LGC, ~ (2007)
  26. X. Xiang, , Anal. Chem, 68, pp.3726~3731 (1996)
  27. Seonghee Ahn, "Analysis of Benzoic Acid in Quasi-Drug Drink Using Isotope Dilution Liquid Chromatography Mass Spectrometry", Bulletin of the Korean Chemical Society, 29(11), pp.2125~2128 (2008), 대한화학회
  28. Y. Lee, , Mass Spectrom. Letters, 2, pp.37~40 (2011)
  29. B. Kim, , Anal. Bioanal. Chem, 398, pp.1035~1042 (2010)
  30. "BIPM, IEC, IFCC, ISO, IUPAC and OIML Guide to the Expression of Uncertainty in Measurement", International Organization for Standardization, ~ (1995)
  31. Byungjoo Kim, "Development of a Model System of Uncertainty Evaluations for Multiple Measurements by Isotope Dilution Mass Spectrometry: Determination of Folic Acid in Infant Formula", Bulletin of the Korean Chemical Society, 31(11), pp.3139~3144 (2010), 대한화학회

관련 논문AI추천

동위원소희석 질량분석법을 이용한 간장 중의 안식향산, 메틸파라벤, 부틸파라벤의 분석안성희한국분석과학회 · 분석과학2018 · 제31권 제6호LC-MS/MS를 이용한 소시지 중 보존료 동시분석김용환, 김은선, 김지연, 서두리, 신지현, 임진택, 장미선, 정보람, 고바라다한국동물위생학회 · 한국동물위생학회지2015 · 제38권 제2호동위원소희석 액체크로마토그래피 질량분석법에 의한 분유 내 콜레스테롤의 정량김병주, 안은정, 이계호, 이화심한국분석과학회 · 분석과학2015 · 제28권 제6호동위원소희석 액체크로마토그래피/질량분석법에 의한 혈청 내 콜레스테롤의 정량신혜선, 이계호, 이화심한국분석과학회 · 분석과학2008 · 제21권 제6호식품 중 제초제 클로피랄리드(Clopyralid)의 잔류 분석법김도훈, 김종수, 김지영, 김효진, 도정아, 이강봉, 정용현, 최윤주한국환경농학회 · 한국환경농학회지2018 · 제37권 제4호동위원소희석 액체크로마토그래피-질량분석법을 이용한 육류중 클로람페니콜의 정밀한 정량 분석에 관한 연구정혁, 김병주, 송혜민, 안성희한국분석과학회 · 분석과학2010 · 제23권 제6호

이 간행물의 다른 논문 (11건)

졸-겔법으로 합성된 ZrO2-SiO2 유리전구체의 결정화구조에 미치는 열처리의 영향분석전경수pp. 3-6제주도 고산지역의 라돈 배경농도 실시간 모니터링허철구, 김원형, 강창희, 강동훈pp. 7-13라돈 추적자를 이용한 유류오염에 대한 연구고경석, 고동찬, 이길용, 조수영, 윤윤열pp. 14-18인체 혈장 및 소변 중 에르타페넴의 정량을 위한 LC-MS/MS 분석법 검증임용진, 황민호, 채수완, 김윤정, 김민걸, 한송희, 전지영pp. 19-24콘크리트 내 Friedel염의 XRD를 이용한 반정량적 측정기법에 관한 연구이호재, 김도겸, 이장화pp. 33-38
원문 보기