세프록사딘 캡슐 시험에 사용되는 유해시약인사염화탄소 대체 공정시험법 개발

Abstract

Currently oxidation-reduction titration method for the assay method using the hazardous reagent,carbon tetrachloride, for cefroxadine capsules has been used in Korean Pharmaceutical Codex. We developedand validated an alternative HPLC assay method by substituting the use of the hazardous reagent, carbontetrachloride, to the use of less toxic ones like acetonitrile. The linearity of the calibration curves in the desiredconcentration range was good (r2 > 0.999). Relative standard deviations of intra-day precision were rangedbetween 0.30% and 0.69% and inter-day precision were ranged between 0.47% and 0.82%. Accuracy wasobtained with recoveries in range of 100.20% and 100.56%. Developed method was applied to the determinationof cefroxadine in commercial cefroxadine capsule and could be expected to become valuable tools for revisingthe Korean Pharmaceutical Codex.